研究报告

雷蒙德氏棉和亚洲棉基因组数据中LPAAT基因家族的发掘及其同源基因在陆地棉材料中的表达分析

展开
  • 1. 中国农业科学院棉花研究所,棉花生物学国家重点实验室,安阳 455000;
    2. 西北农林科技大学农学院,杨凌 712100
马建江,在读硕士研究生,研究方向:棉花遗传育种。E-mail: 504569362@qq.com

收稿日期: 2015-01-05

  修回日期: 2015-05-14

  网络出版日期: 2015-07-20

基金资助

国家高技术研究发展计划项目(863计划)(编号2013AA102601-01-14)和国家自然科学基金项目(编号31301368)资助

Genome-wide analysis of the LPAAT gene family in Gossypium raimondii and G. arboreum, and expression analysis of its orthologs in G. hirsutum

Expand
  • 1. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China;
    2. College of Agronomy, Northwest A&F University, Yangling 712100, China

Received date: 2015-01-05

  Revised date: 2015-05-14

  Online published: 2015-07-20

摘要

溶血磷脂酸酰基转移酶(Lysophosphatidic acid acyltransferase, LPAAT)是油脂合成途径中的一个关键酶,能催化溶血磷脂酸转变为磷脂酸。本研究从雷蒙德氏棉(G. raimondii, D5)和亚洲棉(G. arboreum, A2)的基因组数据中得到17个LPAAT基因家族成员。利用生物信息学方法对二倍体棉花LPAAT基因进行基因结构、染色体分布以及系统进化分析。结果表明,LPAAT基因家族根据亲缘关系的远近可以分为不同的亚家族,各亚家族中LPAAT基因具有相似的基因结构;LPAAT家族基因编码的氨基酸序列具有3个保守基序,其中包括ΦFPEGTR-G结合位点和Φ-NHQS-ΦDΦΦ催化位点;通过对不同物种的LPAAT基因家族进行系统进化分析可知,不同物种中的LPAAT在进化中存在较大差异。基于陆地棉(G. hirsutum)不同发育时期的胚珠RNA-seq数据库和qRT-PCR表达分析,发现LPAAT基因可能对脂肪积累起到积极作用。本研究结果有助于了解棉属植物LPAAT基因家族的功能,以期从中选取较好的LPAAT基因进行进一步功能验证。

本文引用格式

马建江, 王诺菡, 吴嫚, 裴文锋, 王文魁, 李兴丽, 张金发, 喻树迅, 于霁雯 . 雷蒙德氏棉和亚洲棉基因组数据中LPAAT基因家族的发掘及其同源基因在陆地棉材料中的表达分析[J]. 遗传, 2015 , 37(7) : 692 -701 . DOI: 10.16288/j.yczz.15-010

Abstract

Lysophosphatidic acid acyltransferase (LPAAT) which converts lysophosphatidic acid into phosphatidic acid is a key enzyme in biosynthesis pathway of lipid in plants. In this study, we identified 17 members of the LPAAT gene family from genomic data of G. raimondii-D5 and G. arboreum-A2. Analysis of gene structure, chromosome distribution and phylogenetic evolution of LPAAT genes in diploid Gossypium using bioinformatics approaches showed that these genes can be divided into distinct subfamilies based on the distance of their genetic relationship. Moreover, the gene structures were similar within LPAAT subfamily members. The amino acid sequences encoded by LPAAT family genes contained three conserved motifs, including ΦFPEGTR-G binding site and Φ-NHQS- ΦDΦΦ catalytic site. Phylogenetic analysis of LPAAT gene family demonstrated significant differences in evolution of LPAAT in different species. Finally, expression analysis of G. hirsutum ovules in different stages from RNA-seq and qRT-PCR data indicated that LPAAT gene may play a positive role in oil accumulation. Our studies facilitate understanding of the function of LPAAT gene family in Gossypium and selecting better LPAAT genes for further functional validation.

参考文献

[1] 王彦霞, 刘正杰, 马峙英, 李召虎, 华金平. RNA干涉技术与棉花高油育种. 棉花学报, 2011, 23(2): 178-183.
[2] 刘明, 范君华, 由宝昌, 梅拥军. 海岛棉棉仁蛋白质和脂肪含量分析. 作物品种资源, 1994, (4): 25-28.
[3] 喻树迅, 于霁雯. 棉花作为能源作物的可行性分析. 见: 中华人民共和国农业部编. 中国农村生物质能源国际研讨会暨东盟与中日韩生物质能源论坛论文集. 北京: 中国农业出版社, 2008: 369.
[4] 陈四龙, 黄家权, 雷永, 任小平, 文奇根, 陈玉宁, 姜慧芳, 宴立英, 廖伯寿. 花生溶血磷脂酸酰基转移酶基因的克隆与表达分析. 作物学报, 2012, 38(2): 245-255.
[5] Weselake RJ, Taylor DC, Rahman MH, Shah S, Laroche A, McVetty PBE, Harwood JL. Increasing the flow of carbon into seed oil. Biotechnol Adv , 2009, 27(6): 866-878.
[6] Lewin TM, Wang P, Coleman R A. Analysis of amino acid motifs diagnostic for the sn-glycerol-3-phosphate acyltransferase reaction. Biochemistry , 1999, 38(18): 5764-5771.
[7] Heath RJ, Rock CO. A conserved histidine is essential for glycerolipid acyltransferase catalysis. J Bacteriol , 1998, 180(6): 1425-1430.
[8] Kim HU, Huang AHC. Plastid lysophosphatidyl acyltransferase is essential for embryo development in Arabidopsis. Plant Physiol , 2004, 134(3): 1206-1216.
[9] 尹永泰. 甘蓝型油菜溶血磷脂酸酰基转移酶基因的克隆和表达[学位论文]. 武汉: 华中科技大学, 2011.
[10] 华方静, 刘风珍, 万勇善, 张昆. 花生及其野生种质溶血磷脂酸酰基转移酶基因( LPAAT )的克隆及序列分析. 分子植物育种, 2014, 12(1): 74-79.
[11] Cagliari A, Margis-Pinheiro M, Loss G, Mastroberti AA, de Araujo Mariath JE, Margis R. Identification and expression analysis of castor bean ( Ricinus communis ) genes encoding enzymes from the triacylglycerol biosynthesis pathway. Plant Sci , 2010, 179(5): 499-509.
[12] Knutzon DS, Lardizabal KD, Nelsen JS, Bleibaum JL, Davies HM, Metz JG. Cloning of a coconut endosperm cDNA encoding a 1-acyl-sn-glycerol-3- phosphate acyltransferase that accepts medium-chain-length substrates. Plant Physiol , 1995, 109(3): 999-1006.
[13] Taylor DC, Francis T, Lozinsky S, Hoffman T, Giblin M, Marillia EF. Cloning and characterization of a constitutive lysophosphatidic acid acyltransferase 2 (LPAT2) gene from Tropaeolum majus L. Open Plant Sci J , 2010, 4: 7-17.
[14] Brown AP, Carnaby S, Brough C, Brazier M, Slabas AR. Limnanthes douglasii lysophosphatidic acid acyltransferases: immunological quantification, acyl selectivity and functional replacement of the Escherichia coli plsC gene. Biochem J , 2002, 364: 795-805.
[15] Brown AP, Coleman J, Tommey AM, Watson MD, Slabas AR. Isolation and characterisation of a maize cDNA that complements a 1-acyl sn-glycerol-3-phosphate acyltransferase mutant of Escherichia coli and encodes a protein which has similarities to other acyltransferases. Plant Mol Biol , 1994, 26(1): 211-223.
[16] Zou J, Katavic V, Giblin EM, Barton DL, MacKenzie SL, Keller WA, Hu X, Taylor DC. Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell , 1997, 9(6): 909-923.
[17] Maisonneuve S, Bessoule JJ, Lessire R, Delseny M, Roscoe TJ. Expression of rapeseed microsomal lysophosphatidic acid acyltransferase isozymes enhances seed oil content in Arabidopsis. Plant Physiol , 2010, 152(2): 670-684.
[18] 张毓婷, 王敏华, 陈家栋, 戎均康, 丁明全. 雷蒙德氏棉HSP70基因家族的进化分析及其同源基因在陆地棉中的表达分析. 遗传, 2014, 36(9): 921-933.
[19] Li FG, Fan GY, Wang KB, Sun FM, Yuan YL, Song GL, Li Q, Ma ZY, Lu CR, Zou CS, Chen WB, Liang XM, Shang HH, Liu WQ, Shi CC, Xiao GH, Gou CY, Ye WW, Xu X, Zhang XY, Wei HL, Li ZF, Zhang GY, Wang JY, Liu K, Kohel RJ, Percy RG, Yu JZ, Zhu YX, Wang J, Yu SX. Genome sequence of the cultivated cotton Gossypium arboreum . Nat Genet , 2014, 46(6): 567-572.
[20] Wang KB, Wang ZW, Li FG, Ye WW, Wang JY, Song GL, Yue Z, Cong L, Shang HH, Zhu SL, Li Q, Yuan YL, Lu CR, Wei HL, Gou CY, Zheng ZQ, Yin Y, Zhang XY, Liu K, Wang B, Song C, Shi N, Kohe RJ, Percy RG, Yu JZ, Zhu YX, Wang J, Yu SX. The draft genome of a diploid cotton Gossypium raimondii . Nat Genet , 2012, 44(10): 1098-1103.
[21] Paterson AH, Wendel JF, Gundlach H, Guo H, Jenkins J, Jin DC, Llewellyn D, Showmaker KC, Shu SQ, Udall J, Yoo MJ, Byers R, Chen W, Doron-Faigenboim A, Duke MV, Gong L, Grimwood J, Grover C, Grupp K, Hu GJ
文章导航

/