研究报告

杂交粳稻花优14及其亲本孕穗期剑叶的基因表达谱芯片分析

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  • 1. 上海市农业科学院,作物育种栽培研究所,上海 201403;
    2. 复旦大学生命科学学院,上海 200433
储黄伟,博士,助理研究员,研究方向:植物分子生物学。E-mail:chuhuangwei@saas.sh.cn

收稿日期: 2015-03-03

  修回日期: 2015-07-29

  网络出版日期: 2015-09-20

基金资助

上海市科技兴农重点攻关项目(编号:沪农科攻字(2014)第7-1-2号和沪农科攻字(2013)第1-3号)和上海市科委农业科技攻关项目; (编号:14395810601)资助

Gene expression profiling of flag leaves at the booting stage in the japonica hybrid rice Huayou14 and its parental lines by microarray

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  • 1. Institute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China;
    2. School of Life Sciences, Fudan University, Shanghai 200433, China

Received date: 2015-03-03

  Revised date: 2015-07-29

  Online published: 2015-09-20

摘要

本文以孕穗期的杂交粳稻花优14及其母本申9A和父本繁14的剑叶为材料,利用Affymetrix水稻基因组芯片检测了3个样品中的基因表达谱,并用生物信息学方法对差异表达基因进行了分析。结果表明:与其亲本相比,杂交粳稻花优14中共有2057个基因的表达水平出现了2倍(变化倍数≥2或≤0.5)以上的变化。通过对这些差异表达基因进行GO(Gene Ontology)功能分类,发现差异表达基因在光合系统Ⅰ、叶绿体膜和叶绿体被膜等与叶绿体相关的细胞组分中显著富集;同时差异表达基因还在叶绿素合成、叶绿素代谢和类胡萝卜素合成等生物学过程中富集。光合作用效率的改变可能和花优14杂种优势的形成相关。与已报道结果不同,本文在代谢途径分析结果中并没有发现花优14中差异表达基因在碳固定和光合作用等途径中显著的富集,但是发现差异表达基因在光合作用-天线蛋白以及淀粉和蔗糖的代谢途径中显著富集。该结果表明,在不同的杂交组合中,参与杂种优势形成的基因或者代谢途径可能是不同的。

本文引用格式

储黄伟, 牛付安, 程灿, 周继华, 王新其, 罗小金, 袁勤, 曹黎明 . 杂交粳稻花优14及其亲本孕穗期剑叶的基因表达谱芯片分析[J]. 遗传, 2015 , 37(9) : 932 -938 . DOI: 10.16288/j.yczz.15-094

Abstract

Gene expression profiling using microarray has contributed significantly to heterosis studies. Using the Affymetrix rice genome array, we investigated gene expression profiles in the flag leaves of the japonica hybrid rice Huayou14 and its parental cultivars Shen9A and Fan14 at the booting stage. A total of 2057 genes differentially expressed (fold change ≥2 or ≤0.5) between Huayou14 and its parents were identified. Functional classification of the differentially expressed genes by Gene Ontology (GO) analysis indicated the differentially expressed genes were significantly enriched in photosynthesis-related cellular component categories (e.g. photosystem Ⅰ, chloroplast membrane and chloroplast envelope), and biological process categories (e.g. chlorophyll catabolic, chlorophyll biosynthetic and carotenoid biosynthetic processes). These results suggest that the changes in the photosynthetic ability of the japonica hybrid rice Huayou14 may be related to heterosis. Metabolic pathway analysis indicated that differentially expressed genes were significantly enriched in photosynthesis-antenna proteins and starch and sucrose metabolic pathways, instead of photosynthesis and carbon fixation pathways as reported previously. These results suggest that different genes or metabolic pathways might contribute to the heterosis of different hybrid combinations.

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