NAC是植物特有的具有多种功能的一类转录因子,广泛参与植物的生长发育,器官建成及抗逆境胁迫等反应.目前有关NAC转录因子的研究主要针对模式植物(如拟南芥和水稻),而在小麦中的研究相对较少.本文利用生物信息学方法,获得乌拉尔图小麦(Triticum urartu)NAC转录因子家族基因的全长序列,并对其进化关系,生物学功能,染色体定位以及基因复制等进行预测与分析,同时利用荧光定量PCR验证相关转录因子在非生物胁迫下的表达模式.结果显示,共筛选得到87个乌拉尔图小麦全长NAC转录因子,通过进化树分析将其分为7个亚族,其中39个NAC 转录因子被定位在7条染色体上.通过基因复制分析发现,有5对NAC转录因子基因发生了复制.进一步通过荧光定量验证4个NAC转录因子在非生物胁迫下的表达模式,发现4个转录因子均受不同胁迫而上调表达.
[1] Souer E, van Houwelingen A, Kloos D, Mol J, Koes R. The No apical meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordial boundaries. Cell , 1996, 85(2): 159-170.
[2] Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M. Genes involved in organ separation in Arabidopsis : an analysis of the cup-shaped cotyledon mutant. Plant Cell , 1997, 9(6): 841-857.
[3] Kikuchi K, Ueguchi-Tanaka M, Yoshida KT, Nagato Y, Matsusoka M, Hirano HY. Molecular analysis of the NAC gene family in rice. Mol Gen Genet , 2000, 262(6): 1047-1051.
[4] Huang QJ, Wang Y, Li B, Chang JL, Chen MJ, Li KX, Yang GX, He GY. TaNAC29, a NAC transcription factor from wheat, enhances salt and drought tolerance in transgenic Arabidopsis . BMC Plant Biology , 2015, 15(1): 268.
[5] Liu ZJ, Shao FX, Tang GY, Shan L, Bi YP. Cloning and characterization of a transcription factor ZmNAC1 in maize ( Zea mays ). Hereditas (Beijing) , 2009, 31(2): 199-205. 柳展基, 邵凤霞, 唐桂英, 单雷, 毕玉平. 一个新的玉米NAC类基因( ZmNAC1 )的克隆与分析. 遗传, 2009, 31(2): 199-205.
[6] Nuruzzaman M, Manimekalai R, Sharoni AM, Satoh K, Kondoh H, Ooka H, Kikuchi S. Genome-wide analysis of NAC transcription factor family in rice. Gene , 2010, 465(1-2): 30-44.
[7] Ooka H, Satoh K, Doi K, Nagata T, Otomo Y, Murakami K, Matsubara K, Osato N, Kawai J, Carninci P, Hayashizaki Y, Suzuki K, Kojima K, Takahara Y, Yamamoto K, Kikuchi S. Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis thaliana . DNA Res , 2003, 10(6): 239-247.
[8] Vroemen CW, Mordhorst AP, Albrecht C, Kwaaitaai MACJ, de Vries SC. The CUP-SHAPED COTYLEDON3 gene is required for boundary and shoot meristem formation in Arabidopsis . Plant Cell , 2003, 15(7): 1563-1577.
[9] Xie Q, Frugis G, Colgan D, Chua NH. Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development. Genes & Dev , 2000, 14(23): 3024-3036.
[10] Tran LS, Nakashima K, Sakuma Y, Simpson SD, Fujita Y, Maruyama K, Fujita M, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis -element in the early responsive to dehydration stress 1 promoter. Plant Cell , 2004, 16(9): 2481-2498.
[11] Fujita M, Fujita Y, Maruyama K, Seki M, Hiratsu K, Ohme-Takagi M, Tran LSP, Yamaguchi-Shinozaki K, Shinozaki K. A dehydration-induced NAC protein, RD26, is involved in a novel ABA-dependent stress-signaling pathway. Plant J , 2004, 39(6): 863-876.
[12] Fang YJ, You J, Xie KB, Xie WB, Xiong LZ. Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice. Mol Genet Genomics , 2008, 280(6): 547-563.
[13] Hu HH, Dai MQ, Yao JL, Xiao BZ, Li XH, Zhang QF, Xiong LZ. Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci USA , 2006, 103(35): 12987-12992.
[14] Yokotani N, Ichikawa T, Kondou Y, Matsui M, Hirochika H, Iwabuchi M, Oda K. Tolerance to various environmental stresses conferred by the salt-responsive rice gene ONACo63 in transgenic Arabidopsis . Planta , 2009, 229(5): 1065-1075.
[15] Hu HH, You J, Fang YJ, Zhu XY, Qi ZY, Xiong LZ. Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice. Plant Mol Biol , 2008, 67(1-2): 169-181.
[16] Hu RB, Qi G, Kong YZ, Kong DJ, Gao Q, Zhou GK. Comprehensive analysis of NAC domain transcription factor gene family in Populus trichocarpa . BMC Plant Biol , 2010, 10: 145.
[17] Le DT, Nishiyama R, Watanabe Y, Mochida K, Yamaguchi-Shinozaki K, Shinozaki K, Tran LSP. Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress. DNA Res , 2011, 18(4): 263-276.
[18] Wang N, Zheng
