综述

线粒体与多潜能干细胞功能

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  • 内蒙古民族大学动物科技学院,通辽 028043
贾振伟,博士,副教授,研究方向:配子与胚胎生物技术研究。E-mail: zhenwei1999@sina.com

收稿日期: 2016-03-24

  网络出版日期: 2016-07-20

基金资助

内蒙古自治区自然科学基金项目(编号:2015MS0304)资助 [Supported by the Natural Science Foundation of Inner Mongolia Autonomous Region of China (No; 2015MS0304)]

Mitochondria and pluripotent stem cells function

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  • College of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao 028043, China

Received date: 2016-03-24

  Online published: 2016-07-20

摘要

线粒体是细胞内重要的细胞器,主要功能是通过氧化磷酸化为细胞生命活动提供能量。近年来,研究表明,在多潜能干细胞(Pluripotent stem cells, PSCs)中线粒体表现出独有的特征,即在多能性状态下,PSCs主要依靠糖酵解提供能量,其分化期间线粒体氧化磷酸化代谢能力逐渐增强。相反,体细胞重编程为多潜能干细胞期间,线粒体氧化磷酸化向糖酵解途径的转变是其成功重编程必需的代谢过程。另外,线粒体通过生物合成和形态结构的动态重塑维持了PSCs多能性、诱导分化及诱导多能干细胞(Induced pluripotent stem cells, iPSCs)的重编程。因此,本文综述了PSCs线粒体形态结构及其在调控PSCs多能性、合成代谢、氧化还原状态的平衡、分化及重新编程中的作用,为深入了解线粒体调控PSCs功能的作用提供理论基础。

本文引用格式

贾振伟 . 线粒体与多潜能干细胞功能[J]. 遗传, 2016 , 38(7) : 603 -611 . DOI: 10.16288/j.yczz.16-001

Abstract

Mitochondria are important intracellular organelles which provide energy for cellular activities through oxidative phosphorylation. Recently, mitochondria have been shown to exhibit peculiar features in pluripotent stem cells (PSCs), namely, PSCs rely mainly on glycolysis for energy supply in pluripotent states while mitochondrial oxidative phosphorylation function is gradually enhanced during PSCs differentiation. In contrast, during somatic reprogramming, the metabolic transition from mitochondrial oxidative phosphorylation to glycolysis is necessary for successful reprogramming. Moreover, mitochondrial biogenesis and dynamics are also actively involved in the maintenance of pluripotency, induction of differentiation and induced pluripotent stem cells (iPSCs) reprogramming. Here we reviewed mitochondrial structure and function in regulating PSCs pluripotency, anabolism, redox homeostasis, differentiation, and reprogramming, which may provide reference for further understanding the role of mitochondria in PSCs.

参考文献

[1] Ren CF, Sun HY, Wang LZ, Zhang GM, Fan YX, Yan GY, Wang D, Wang F. Reprogramming mechanism and genetic stability of induced pluripotent stem cells (iPSCs). Hereditas (Beijing) , 2014, 36(9): 879-887. 任才芳, 孙红艳, 王立中, 张国敏, 樊懿萱, 颜光耀, 王丹, 王锋. iPSCs遗传稳定性与重编程机制的研究进展. 遗传, 2014, 36(9): 879-887.
[2] Wanet A, Arnould T, Najimi M, Renard P. Connecting mitochondria, metabolism, and stem cell fate. Stem Cells Dev , 2015, 24(17): 1957-1971.
[3] Teslaa T, Teitell MA. Pluripotent stem cell energy metabolism: an update. EMBO J , 2015, 34(2): 138-153.
[4] Bukowiecki R, Adjaye J, Prigione A. Mitochondrial function in pluripotent stem cells and cellular reprogramming. Gerontology , 2014, 60(2): 174-182.
[5] Prigione A, Rohwer N, Hoffmann S, Mlody B, Drews K, Bukowiecki R, Blümlein K, Wanker EE, Ralser M, Cramer T, Adjaye J. HIF1α modulates cell fate reprogramming through early glycolytic shift and upregulation of PDK1-3 and PKM2. Stem Cells , 2014, 32(2): 364-376.
[6] Lees JG, Rathjen J, Sheedy JR, Gardner DK, Harvey AJ. Distinct profiles of human embryonic stem cell metabolism and mitochondria identified by oxygen. Reproduction , 2015, 150(4): 367-382.
[7] Kwon IK, Lee SC, Hwang YS, Heo JS. Mitochondrial function contributes to oxysterol-induced osteogenic differentiation in mouse embryonic stem cells. Biochim Biophys Acta , 2015, 1853(3): 561-572.
[8] Hoppins S. The regulation of mitochondrial dynamics. Curr Opin Cell Bio , 2014, 29: 46-52.
[9] Kowno M, Watanabe-Susaki K, Ishimine H, Komazaki S, Enomoto K, Seki Y, Wang YY, Ishigaki Y, Ninomiya N, Noguchi TA, Kokubu Y, Ohnishi K, Nakajima Y, Kato K, Intoh A, Takada H, Yamakawa N, Wang PC, Asashima M, Kurisaki A. Prohibitin 2 regulates the proliferation and lineage-specific differentiation of mouse embryonic stem cells in mitochondria. PLoS One , 2014, 9(4): e81552.
[10] Chung S, Arrell DK, Faustino RS, Terzic A, Dzeja PP. Glycolytic network restructuring integral to the energetics of embryonic stem cell cardiac differentiation. J Mol Cell Cardiol , 2010, 48(4): 725-734.
[11] Prigione A, Fauler B, Lurz R, Lehrach H, Adjaye J. The senescence-related mitochondrial/oxidative stress pathway is repressed in human induced pluripotent stem cells. Stem Cells , 2010, 28(4): 721-733.
[12] Varum S, Rodrigues AS, Moura MB, Momcilovic O, Easley CA, Ramalho-Santos J, Van Houten B, Schatten G. Energy metabolism in human pluripotent stem cells and their differentiated counterparts. PLoS One , 2011, 6(6): e20914.
[13] Lonergan T, Brenner C, Bavister B. Differentiation-related changes in mitochondrial properties as indicators of stem cell competence. J Cell Physiol , 2006, 208(1): 149- 153.
[14] Facucho-Oliveira JM, Alderson J, Spikings EC, Egginton S, St John JC. Mitochondrial DNA replication during differentiation of murine embryonic stem cells. J Cell Sci , 2007, 120(Pt 22): 4025-4034.
[15] Birket MJ, Orr AL, Gerencser AA, Madden DT, Vitelli C, Swistowski A, Brand MD, Zeng XM. A reduction in ATP demand and mitochondrial activity with neural differentiation of human embryonic stem cells. J Cell Sci , 2011, 124(Pt 3): 348-358.
[16] Zhang J, Khvorostov I, Hong JS, Oktay Y, Vergnes L, Nuebel E, Wahjudi PN, Setoguchi K, Wang G, Do AN, Jung HJ, McCaffery JM, Kurland IJ, Reue K, Lee WN, Koehler CM, Teitell MA. UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells. EMBO J , 2011, 30(24): 4860-4873.
[17] Suhr ST, Chang EA, Tjong J, Alcasid N, Perkins GA, Goissis MD, Ellisman MH, Perez GI, Cibelli JB. Mitochondrial rejuvenation after induced pluripotency. PLoS One , 2010, 5(11): e14095.
[18] Folmes CDL, Nelson TJ, Martinez-Fernandez A, Arrell DK, Lindor JZ, Dzeja PP, Ikeda Y, Perez-Terzic C, Terzic A. Somatic oxidative bioenergetics transitions into pluripotency-dependent glycolysis to facilitate nuclear reprogramming. Cell Metab , 2011, 14(2): 264-271.
[19] Chen TT, Shen L, Yu J, Wa
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