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群体感应与微生物耐药性

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  • 1. 华南农业大学群体微生物研究中心,广州 510642;
    2. 广东省微生物信号与作物病害防控重点实验室,广州 510642
张炼辉,博士,教授,博士生导师,研究方向:微生物学、植物病理学。

收稿日期: 2016-04-20

  修回日期: 2016-06-20

  网络出版日期: 2016-10-20

基金资助

国家重点基础研究发展计划项目(973计划)(编号:2015CB150600)和国家自然科学基金项目(编号:31330002,31270170)资助

Quorum sensing and microbial drug resistance

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  • 1. Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China;
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, China

Received date: 2016-04-20

  Revised date: 2016-06-20

  Online published: 2016-10-20

Supported by

[Supported by the National Basic Research Program of China (973 Program) (No; 2015CB150600) and the National Natural Science Foundation of China (Nos; 31330002, 31270170)]

摘要

微生物耐药性已成为全球关注的严重问题,其演化机制和调控机理也已成为研究热点。近年来的研究发现,一些微生物耐药性机制受到群体感应系统的调控。群体感应是一种在微生物界广泛存在并与菌体密度关联的细胞-细胞间的通讯系统。高密度的菌落群体能够产生足够数量的小分子信号,激活下游包括致病毒力和耐药性机制在内的多种细胞进程,耐受抗生素并且危害寄主。本文结合国内外最新的研究进展,对微生物群体感应系统的研究现状进行了概括性介绍,重点阐述了群体感应系统对微生物耐药性机制的调控作用,如微生物生物被膜形成和药物外排泵调控等方面的作用,并探讨了利用群体淬灭控制微生物耐药性的新策略。

本文引用格式

陈昱帆, 刘诗胤, 梁志彬, 吕明发, 周佳暖, 张炼辉 . 群体感应与微生物耐药性[J]. 遗传, 2016 , 38(10) : 881 -893 . DOI: 10.16288/j.yczz.16-141

Abstract

Microbial drug resistance has become a serious problem of global concern, and the evolution and regulatory mechanisms of microbial drug resistance has become a hotspot of research in recent years. Recent studies showed that certain microbial resistance mechanisms are regulated by quorum sensing system. Quorum sensing is a ubiquitous cell-cell communication system in the microbial world, which associates with cell density. High-density microbial cells produce sufficient amount of small signal molecules, activating a range of downstream cellular processes including virulence and drug resistance mechanisms, which increases bacterial drug tolerance and causes infections on host organisms. In this review, the general mechanisms of microbial drug resistance and quorum-sensing systems are summarized with a focus on the association of quorum sensing and chemical signaling systems with microbial drug resistance mechanisms, including biofilm formation and drug efflux pump. The potential use of quorum quenching as a new strategy to control microbial resistance is also discussed.

参考文献

[1] Morens DM, Folkers GK, Fauci AS. The challenge of emerging and re-emerging infectious diseases. Nature , 2004, 430(6996): 242-249.
[2] Wise R. Antimicrobial resistance: priorities for action. J Antimicrob Chemother , 2002, 49(4): 585-586.
[3] Monroe S, Polk R. Antimicrobial use and bacterial resistance. Curr Opin Microbiol , 2000, 3(5): 496-501.
[4] Livermore DM. The need for new antibiotics. Clin Microbiol Infect , 2004, 10(Suppl. 4): 1-9.
[5] Andersson DI, Hughes D. Antibiotic resistance and its cost: is it possible to reverse resistance? Nat Rev Microbiol , 2010, 8(4): 260-271.
[6] Spratt BG. Resistance to antibiotics mediated by target alterations. Science , 1994, 264(5157): 388-393.
[7] Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet , 2001, 358(9276): 135-138.
[8] Barbier F, Wolff M. Multi-drug resistant Pseudomonas aeruginosa : towards a therapeutic dead end? Med Sci ( Paris ), 2010, 26(11): 960-968.
[9] Fuqua C, Greenberg EP. Listening in on bacteria: acyl- homoserine lactone signalling. Nat Rev Mol Cell Biol , 2002, 3(9): 685-695.
[10] Deng YY, Wu JE, Tao F, Zhang LH. Listening to a new language: DSF-based quorum sensing in gram-negative bacteria. Chem Rev , 2011, 111(1): 160-173.
[11] Walsh C. Molecular mechanisms that confer antibacterial drug resistance. Nature , 2000, 406(6797): 775-781.
[12] Zhou JN, Zhang HB, Lv MF, Chen YF, Liao LS, Cheng YY, Liu SY, Chen SH, He F, Cui ZN, Jiang ZD, Chang CQ, Zhang LH. SlyA regulates phytotoxin production and virulence in Dickeya zeae EC1. Mol Plant Pathol , 2016, doi:10.1111/mpp.12376.
[13] Ambler RP, Coulson AFW, Frère JM, Ghuysen JM, Joris B, Forsman M, Levesque RC, Tiraby G, Waley SG. A standard numbering scheme for the class A β -lactamases. Biochem J , 1991, 276(1): 269-270.
[14] Bush K, Jacoby GA, Medeiros AA. A functional classification scheme for β-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother , 1995, 39(6): 1211-1233.
[15] Medeiros A A. β-lactamases. Br Med Bull , 1984, 40(1): 18-27.
[16] Philippon A, Labia R, Jacoby G. Extended-spectrum β-lactamases. Antimicrob Agents Chemother , 1989, 33(8): 1131-1136.
[17] Ramirez MS, Tolmasky ME. Aminoglycoside modifying enzymes. Drug Resist Updat , 2010, 13(6): 151-171.
[18] Levy SB. Active efflux mechanisms for antimicrobial resistance. Antimicrob Agents Chemother , 1992, 36(4): 695-703.
[19] Paulsen IT, Brown MH, Skurray RA. Proton-dependent multidrug efflux systems. Microbiol Mol Biol Rev , 1996, 60(4): 575-608.
[20] Tipper DJ, Strominger JL. Mechanism of action of penicillins: a proposal based on their structural similarity to acyl-D-alanyl-D-alanine. Proc Natl Acad Sci USA , 1965, 54(4): 1133-1141.
[21] Song MD, Wachi M, Doi M, Ishino F, Matsuhashi M. Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. FEBS Lett , 1987, 221(1): 167-171.
[22] Ubukata K, Nonoguchi R, Matsuhashi M, Konno M. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S . aureus -specific penicillin-binding protein. J Bacteriol , 1989, 171(5): 2882-2885.
[23] Peacock SJ, Paterson GK. Mechanisms of methicillin resistance in Staphylococcus aureus . Annu Rev Biochem , 2015, 84(1): 577-601.
[24] Lim D, Strynadka NCJ. Structural basis for the β lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus . Nat Struct Biol , 2002, 9(11): 870-876.
[25] Mah TFC, O'Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol , 2001, 9(1): 34-39.
[26] Poole K. Efflux-mediated multiresistance in Gram-negative bacteria. Clin Microbiol Infect , 2004, 10(1): 12-26.
[27] de la Cruz F, Davies J. Horizontal gene transfer and the origin of spec
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