通过细胞学方法和染色体特异分子标记鉴定六倍体小偃麦(AABBEE)与硬粒小麦(AABB)杂交的自交后代F2和F3植株,探讨长穗偃麦草染色体在硬粒小麦背景中世代间的传递特征,并筛选硬粒小麦-长穗偃麦草E染色体附加系。对218个F2单株染色体数检测表明,2n=28植株占41.7%,2n=29植株占18.3%,其余40.0%植株的染色体数在2n=31~42范围内。分子标记鉴定表明,在F2代2n=29单体附加植株中,不同的长穗偃麦草染色体传递率之间存在明显差异,1E传递率最高,3E和6E传递率最低。在F2代2n=30单株中,1E、4E、7E和5E染色体相互组合产生的双单体多,6E参与组合较少,未检测到2E或3E与其他染色体的组合单株。在1E~7E单体附加株自交后代F3中,E染色体传递率变化范围为9.1%~27.5%,1E传递率最高,6E传递率最低,与F2的传递率一致。从F3代中选育出1E~7E单体附加及少数二体附加,所有单体附加均可育。这些附加E染色体材料将对小麦代换系和易位系的创制提供有益的中间材料。
李海凤, 刘慧萍, 戴毅, 黄帅, 张军, 高勇, 陈建民
. 四倍体小麦背景中长穗偃麦草E染色体传递特征[J]. 遗传, 2016
, 38(11)
: 1020
-1029
.
DOI: 10.16288/j.yczz.16-107
The transmission patterns of Thinopyrum elongatum chromosomes in the background of Triticum turgidum were investigated through cytogenetic and molecular marker analysis based on the F2 and F3 plants derived from a cross between Triticum trititrigia (AABBEE) and T. turgidum L. ssp. durum (AABB). An additional objective was to develop durum-Th. elongatum E chromosome addition lines. Among 218 F2 plants, individuals with 2n=28 accounted for 41.7%, those with 2n=29 accounted for 18.3%, and the remaining 40.0% had 2n=31-42. Molecular marker analysis of the F2 monosomic addition plants with 2n=29 showed significant differences in transmission rate among Th. elongatum chromosomes. Chromosome 1E had the highest transmission rates, while 3E and 6E had the lowest. Among F2 double monosomic addition plants with 2n=30, joint transmission was frequent between 1E, 4E, 7E and 5E; 6E was less frequent in joint transmission with other chromosomes, while 2E and 3E never transmitted along with other chromosomes. Among F3 plants derived from F2 monosomic addition plants, the transmission rates of E chromosomes varied widely, from the minimum of 9.1% for 6E to the maximum of 27.5% for 1E, consistent with observations from F2 plants. A full set of durum-Th. elongatum 1E-7E monosomic addition lines and several disomic addition lines were selected from F3 plants, and all monosomic addition plants were fertile. These chromosome E addition lines will be useful intermediate materials for developing substitution lines and translocation lines.
[1] Guo HJ, Chen F, Dong ZD, Cui DQ. Allelic variations of phytoene synthase genes controlling yellow pigment content in durum wheat ( Triticum turgidum L.). Acta Agronom Sin , 2011, 37(5): 924-928. 郭慧娟, 陈锋, 董中东, 崔党群. 硬粒小麦品种八氢番茄红素合酶基因等位变异的分子检测. 作物学报, 2011, 37(5): 924-928.
[2] Qi LL, Friebe B, Zhang P, Gill BS. Homoeologous recombination, chromosome engineering and crop improvement. Chromosome Res , 2007, 15(1): 3-19.
[3] Friebe B, Jiang JM, Raupp WJ, McIntosh RA, Gill BS. Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica , 1996, 91(1): 59-87.
[4] Ceoloni C, Forte P, Gennaro A, Micali S, Carozza R, Bitti A. Recent developments in durum wheat chromosome engineering. Cytogenet Genome Res , 2005, 109(1-3): 328-344.
[5] Jauhar PP, Peterson TS. Registration of DGE-1, a durum alien disomic addition line with resistance to Fusarium head blight. J Plant Regist , 2008, 2(2): 167-168.
[6] Jauhar PP, Peterson TS, Xu SS. Cytogenetic and molecular characterization of a durum alien disomic addition line with enhanced tolerance to Fusarium head blight. Genome , 2009, 52(5): 467-483.
[7] Jauhar PP. Durum wheat genetic stocks involving chromosome 1E of diploid wheatgrass: resistance to Fusarium head blight. Nucleus , 2014, 57(1): 19-23.
[8] Forte P, Virili ME, Kuzmanović L, Moscetti I, Gennaro A, D’Ovidio R, Ceoloni C. A novel assembly of Thinopyrum ponticum genes into the durum wheat genome: pyramiding Fusarium head blight resistance onto recombinant lines previously engineered for other beneficial traits from the same alien species. Mol Breed , 2014, 34(4): 1701-1716.
[9] Kuzmanovic L, Gennaro A, Benedettelli S, Dodd IC, Quarrie SA, Ceoloni C. Structural-functional dissection and characterization of yield-contributing traits originating from a group 7 chromosome of the wheatgrass species Thinopyrum ponticum after transfer into durum wheat. J Exp Bot , 2014, 65(2): 509-525.
[10] Li JL, Hu ZM. The establishment of clones of Triticum durum × Elytrigia glauca intergenetic hybrid. Hereditas ( Beijing ), 1990, 12(3): 1-3. 李集临, 胡赞民. 硬粒小麦×天蓝偃麦草属间杂种无性系的建立. 遗传, 1990, 12(3): 1-3.
[11] Han FP, Li JL. Morphology and cytogenetics of intergeneric hybrids of crossing Triticum durum and T . timopheevi with tetraploid Elytrigia elongata . Acta Genet Sin , 1993, 20(1): 44-49. 韩方普, 李集临. 硬粒小麦、提莫菲维小麦与四倍体长穗偃麦草属间杂种的形态和细胞遗传学研究. 遗传学报, 1993, 20(1): 44-49.
[12] Deng ZY, Zhang XQ. Evolution of partial promoter region of HMW glutenin genes from E and E 1 genome of Agropyron elongatum . Hereditas ( Beijing ), 2004, 26(3): 325- 329. 邓志勇, 张相歧. 长穗偃麦草中E和E 1 基因组高分子量麦谷蛋白基因启动子部分序列的进化分析. 遗传, 2004, 26(3): 325-329.
[13] Xin WL. Cytogenetic study on crosses between octoploid Trititrigia and hexaploid Trititrigia . Heilongjiang Agric Sci , 1998, (3): 4-7. 辛文利. 八倍体小偃麦与六倍体小偃麦杂交的细胞遗传学研究. 黑龙江农业科学, 1998, (3): 4-7.
[14] Ren ZL, Lelley T, Robbelen G. Transmission of the wheat and Rye chromosomes in Octoploid Triticale ×common wheat populations. Acta Genet Sin , 1991, 18(2): 161-167. 任正隆, Lelley T, Robbelen G. 小麦和黑麦染色体在小黑麦×小麦杂种的不同世代群体中的传递. 遗传学报, 1991, 18(2): 161-167.
[15] Sharp PJ, Cao S, Desai S, Gale MD. The isolation characterization and application in the Triticale of a set of wheat RFLP probes identifying each homoeologous chromosome arm. Theor Appl Genet , 1989, 78(3): 342-348.
[16] Zhu X. Development of E-chromosome specific SCAR markers for Thinopyrum elongatum in Chinese Spring wheat background[Dissertation]. Yangzhou: Yangzhou University, 2011: 29-36. 朱雪. 小麦中国春背景中长穗偃麦草E染色体组SCAR标记发展[学位论文]. 扬州: 扬州大学, 2011: 29-36.
[17] Ge JY, Chen SQ, Gao YY, Zhu X, Gao Y, Huang ZF, Chen JM. Development of Genome-specific molecular markers for Lophopyrum elongatum based on suppression subtractive hybridization. Acta Agronom Sin , 2012, 38(10): 1818-1826. 葛江燕, 陈士强, 高营营, 朱雪, 高勇, 黄泽峰, 陈建民. 基于抑制消减杂交开发长穗偃麦草( Lophopyrum elongatum )特异分子标记. 作物学报, 20
