绿色杜氏藻不同β-胡萝卜素羟化酶基因家族胁迫应答模式研究
收稿日期: 2016-09-22
修回日期: 2016-12-04
网络出版日期: 2017-02-10
基金资助
浙江省科技厅重点科技创新团队项目(2012R10029-07, 2010R50029);宁波科技攻关项目(2013C10018);宁波大学优秀学位论文培育基金资助项目(py2014020)
Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis
Received date: 2016-09-22
Revised date: 2016-12-04
Online published: 2017-02-10
Supported by
Key Scientific and Technological Innovation Team Project of Zhejiang Province Department of Science and Technology(2012R10029-07, 2010R50029);Foundation of Ningbo Science and Technology(2013C10018);the Outstanding (Postgraduate) Dissertation Growth Foundation of Ningbo University(py2014020)
β-胡萝卜素羟化酶(β-carotenoid hydroxylase, CHYB)是植物类胡萝卜素生物合成途径中的一个重要限速酶。本研究对绿色杜氏藻转录组测序数据进行分析,获得2条β-胡萝卜素羟化酶家族基因chyb1和chyb2。采用染色体步移法分别克隆并获得了绿色杜氏藻chyb1和chyb2基因的启动子序列,全长分别为1080 bp(GenBank登录号：KY012338)和1155 bp (GenBank登录号：KY012339)。利用Plantcare软件分析两个启动子的顺式作用元件,结果表明绿色杜氏藻chyb1基因启动子含有与甲基茉莉酸、花生四烯酸、水杨酸等非生物胁迫相关的顺式作用元件,而绿色杜氏藻chyb2基因启动子含有与光照相关的顺式作用元件。通过qRT-PCR分析了绿色杜氏藻CHYB基因家族在不同胁迫下的基因表达水平,结果表明该基因家族的基因表达水平与启动子调控相关,且不同的家族基因应答不同的胁迫。
朱帅旗,龚一富,章丽,俞凯,王何瑜,严小军 . 绿色杜氏藻不同β-胡萝卜素羟化酶基因家族胁迫应答模式研究[J]. 遗传, 2017 , 39(2) : 156 -165 . DOI: 10.16288/j.yczz.16-327
β-carotenoid hydroxylase (CHYB) is an important rate-limiting enzyme in the biosynthesis of plant carotenoid. In this study, chyb1 and chyb2, two gene families in Dunaliella viridis were obtained by RNA-seq. The fragment of promoters of CHYB family genes, 1 080 bp for chyb1 (GenBank No. KY012338) and 1 155 bp for chyb2 (GenBank No. KY012339) were cloned by the Genome Walking Technology, respectively. Cis-acting elements of two promoters were analyzed by Plantcare soft. The results show that the chyb1 gene promoter contains more cis-acting elements in responses to abiotic stresses, such as methyl jasmonate, arachidonic acid, acetylsalicylic acid, and so on. On the other hand, the chyb2 promoter contains more cis-acting elements in response to light stress. qRT-PCR results show that the mRNA expression levels of CHYBs are modulated by their promoters, and different CHYB gene families response to distinct stresses.
Key words： Dunaliella viridis; β-carotenoid hydroxylase; gene family; promoter; stress
| [1] | Liu HY, Xin NH. Research progress on β-carotene. J Sault Chem Ind, 2013, 42(1): 18-21. | |||
| [1] | 刘洪岩, 辛乃宏. β-胡萝卜素的研究进展. 盐业与化工, 2013, 42(1): 18-21. | |||
| [2] | Lin FM, Chen LR, Lin EH, Ke FC, Chen HY, Tsai MJ, Hsiao PW. Compounds from Wedelia chinensis synergistically suppress androgen activity and growth in prostate cancer cells. Carcinogenesis, 2007, 28(12): 2521-2529. | |||
| [3] | Mathews-Roth MM. Beta carotene therapy for erythropoietic protoporphyria and other photosensitivity diseases. Arch Dermatol, 1977, 113(9): 1229-1232. | |||
| [4] | Cui GH, Huang LQ, Tang XJ, Qiu D, Wang XY, Fu GF. Functional genomics studies of Salvia miltiorrhiza I establish cDNA microarray of S. miltiorrhiza. China J Chin Mater Med, 2007, 32(12): 1137-1141. | |||
| [4] | 崔光红, 黄璐琦, 唐晓晶, 邱德, 王学勇, 付桂芳. 丹参功能基因组学研究I-cDNA芯片的构建. 中国中药杂志, 2007, 32(12): 1137-1141. | |||
| [5] | Kato M, Ikoma Y, Matsumoto H, Sugiura M, Hyodo H, Yano M. Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit. Plant Physiol, 2004, 134(2): 824-837. | |||
| [6] | Zhang L, Gong YF, Liu XD, Zhang WQ. Cloning and expression analysis of β-carotene hydroxylase gene (chyb) from Dunaliella salina. J Agric Biotechnol, 2013, 21(8): 920-930. | |||
| [6] | 章丽, 龚一富, 刘晓丹, 张文青. 盐生杜氏藻β-胡萝卜素羟化酶基因(chyb)的克隆及表达分析. 农业生物技术学报, 2013, 21(8): 920-930. | |||
| [7] | Linden H. Carotenoid hydroxylase from Haematococcus pluvialis: cDNA sequence, regulation and functional complementation. Biochim Biophys Acta, 1999, 1446(3): 203-212. | |||
| [8] | Li YT, Huang JC, Sandmann G, Chen F. Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark- grown Chlorella zofingiensis (Chlorophyceae). Planta, 2008, 228(5): 735-743. | |||
| [9] | Li QL, Farre G, Naqvi S, Breitenbach J, Sanahuja G, Bai C, Sandmann G, Capell T, Christou P, Zhu CF. Cloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturation. Transgenic Res, 2010, 19(6): 1053-1068. | |||
| [10] | Bhosale P, Teredesai PV, Jin LH, Ermakov IV, Gellermann W, Bernstein PS. Production of deuterated zeaxanthin by Flavobacterium Multivorum and its detection by resonance Raman and mass spectrometric methods. Biotechnol Lett, 2005, 27(21): 1719-1723. | |||
| [11] | Du H, Wang NL, Cui F, Li XH, Xiao JH, Xiong LZ. Characterization of the β-carotene hydroxylase gene DSM2 conferring drought and oxidative stress resistance by increasing xanthophylls and abscisic acid synthesis in rice. Plant Physiol, 2010, 154(3): 1304-1318. | |||
| [12] | Tian L, Musetti V, Ki
/
www.chinagene.cn
版权所有 © 中国科学院遗传与发育生物学研究所 中国遗传学会
地址：北京市朝阳区北辰西路1号院 中国科学院遗传与发育生物学研究所1号楼 邮政编码：100101 联系电话：010-64807669 E-mail：yczz@genetics.ac.cn 
|