遗传 >

2017 , Vol. 39 >Issue 3: 220 - 231

DOI: https://doi.org/10.16288/j.yczz.16-386

综述

肿瘤液态活检的研究进展及其临床应用

展开
  • 1. 中国科学院北京基因组研究所,中国科学院基因组科学与信息重点实验室,北京 100101
    2. 中国科学院大学,北京 100049
王晨, 硕士研究生,专业方向：遗传学。E-mail: wangchen@big.ac.cn|方向东，博士，研究员，研究方向：干细胞与重要疾病的组学与转化医学。E-mail: fangxd@big.ac.cn

收稿日期: 2016-11-18

  修回日期: 2017-01-19

  网络出版日期: 2017-02-23

基金资助

国家自然科学基金项目(31471115,31401160,81670109)

收起

Research progress on liquid biopsy in oncology and its clinical applications

Expand
  • 1. CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2016-11-18

  Revised date: 2017-01-19

  Online published: 2017-02-23

Supported by

the National Natural Science Foundation of China(31471115,31401160,81670109)

Fold

摘要

液态活检是一类新兴的病理检测手段,其研究内容包括肿瘤循环细胞、肿瘤循环DNA、外泌体等物质,其中的信息为肿瘤患者的个性化医疗提供有力的依据。它凭借无创、便捷等优势在临床应用中展现出广大前景。近年来,随着液态活检研究内容的扩大及其捕获与检测技术的发展,液态活检在临床中的应用日益广泛。本文旨在探讨近年来液态活检的主要研究对象、检测技术以及在临床应用中的发展前景与所面临的挑战,以期为肿瘤患者获得更佳的治疗,推动肿瘤精准医学的发展。

关键词： 液态活检; 肿瘤循环细胞; 肿瘤循环DNA; 二代测序技术

本文引用格式

王晨,李艳明,方向东 . 肿瘤液态活检的研究进展及其临床应用[J]. 遗传, 2017 , 39(3) : 220 -231 . DOI: 10.16288/j.yczz.16-386

Abstract

Liquid biopsy is an emerging and promising detection tool for cancer, with the benefit of being non-invasive and convenient. It analyzes tumor-derived information in the blood or other body fluids including circulating tumour cells (CTCs), circulating tumour DNA (ctDNA) and exosomes. Nowadays, with the expansion of liquid biopsy research contents and the development of capture and detection technologies, liquid biopsy is increasingly utilized in clinical applications, promoting the development of tumor precision medicine. Here, we mainly focus on reviewing the objects and technologies about liquid biopsy, as well as its applications, development and challenges in clinical practices.

Key words： liquid biopsy; CTCs; ctDNA; NGS

参考文献

[1] Sorrells RB. Synovioanalysis (“liquid biopsy”). J Ark Med Soc, 1974, 71(1): 59-62.
[2] Kolostova K, Pinkas M, Jakabova A, Pospisilova E, Svobodova P, Spicka J, Cegan M, Matkowski R, Bobek V. Molecular characterization of circulating tumor cells in ovarian cancer. Am J Cancer Res, 2016, 6(5): 973-980.
[3] TR A. A case of cancer in which cells similar to those in the tumours were seen in the blood after death. Aust Med J, 1869, 14: 146-149.
[4] Massagué J, Obenauf AC. Metastatic colonization by circulating tumour cells. Nature, 2016, 529(7586): 298-306.
[5] Mostert B, Sieuwerts AM, Kraan J, Bolt-de Vries J, van der Spoel P, van Galen A, Peeters DJ, Dirix LY, Seynaeve CM, Jager A, de Jongh FE, Hamberg P, Stouthard JM, Kehrer DF, Look MP, Smid M, Gratama JW, Foekens JA, Martens JW, Sleijfer S. Gene expression profiles in circulating tumor cells to predict prognosis in metastatic breast cancer patients. Ann Oncol, 2015, 26(3): 510-516.
[6] Nagrath S, Jack RM, Sahai V, Simeone DM. Opportunities and challenges for pancreatic circulating tumor cells. Gastroenterology, 2016, 151(3): 412-426.
[7] Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, LeBleu VS, Mittendorf EA, Weitz J, Rahbari N, Reissfelder C, Pilarsky C, Fraga MF, Piwnica-Worms D, Kalluri R. Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. Nature, 2015, 523(7559): 177-182.
[8] Schwarzenbach H, Hoon DSB, Pantel K. Cell-free nucleic acids as biomarkers in cancer patients. Nat Rev Cancer, 2011, 11(6): 426-437.
[9] Heitzer E, Ulz P, Geigl JB. Circulating tumor DNA as a liquid biopsy for cancer. Clin Chem, 2015, 61(1): 112-123.
[10] Diaz LA, Jr., Bardelli A. Liquid biopsies: genotyping circulating tumor DNA. J Clin Oncol, 2014, 32(6): 579-586.
[11] Zill OA, Greene C, Sebisanovic D, Siew LM, Leng J, Vu M, Hendifar AE, Wang Z, Atreya CE, Kelley RK, Van Loon K, Ko AH, Tempero MA, Bivona TG, Munster PN, Talasaz A, Collisson EA. Cell-free DNA next-generation sequencing in pancreatobiliary carcinomas. Cancer Discov, 2015, 5(10): 1040-1048.
[12] Jones S, Zhang XS, Parsons DW, Lin JCH, Leary RJ, Angenendt P, Mankoo P, Carter H, Kamiyama H, Jimeno A, Hong SM, Fu BJ, Lin MT, Calhoun ES, Kamiyama M, Walter K, Nikolskaya T, Nikolsky Y, Hartigan J, Smith DR, Hidalgo M, Leach SD, Klein AP, Jaffee EM, Goggins M, Maitra A, Iacobuzio-Donahue C, Eshleman JR, Kern SE, Hruban RH, Karchin R, Papadopoulos N, Parmigiani G, Vogelstein B, Velculescu VE, Kinzler KW. Core signaling pathways in human pancreatic cancers revealed by global genomic analyses. Science, 2008, 321(5897): 1801-1806.
[13] Murtaza M, Dawson SJ, Tsui DWY, Gale D, Fo
Options
文章导航

/

〈 〉

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

版权所有 © 中国科学院遗传与发育生物学研究所 中国遗传学会
地址：北京市朝阳区北辰西路1号院　中国科学院遗传与发育生物学研究所1号楼　
邮政编码：100101
联系电话：010-64807669
E-mail：yczz@genetics.ac.cn