秀丽隐杆线虫在高校遗传学实验中的应用
收稿日期: 2017-03-02
修回日期: 2017-03-27
网络出版日期: 2017-12-25
基金资助
国家自然科学基金面上项目(31471311, 31671409);深圳市科技创新基金基础研究计划项目(JCYJ20150529152146477)
Utilization of Caenorhabditis elegans in laboratory teaching of genetics
Received date: 2017-03-02
Revised date: 2017-03-27
Online published: 2017-12-25
Supported by
the National Natural Science Foundation of China(31471311, 31671409);Shenzhen Science, Technology and Innovation Fund(JCYJ20150529152146477)
秀丽隐杆线虫(Caenorhabditiselegans)是模式生物中的重要成员之一,因其实验成本低,实验周期短,非常适宜用于高校的遗传学实验教学中。线虫在实验教学中的使用,一方面可以有效地丰富高校实验教学的内容,另一方面也可以很好地激发学生的学习兴趣。本文介绍了线虫在遗传学实验教学中的应用实例,如生活周期观察、单因子杂交、单核苷酸多态性研究、RNA干扰(RNAi)实验等;对实验设置、操作要求、实验相关准备工作等进行了较为细致的描述,为线虫在高校遗传学实验教学中的应用提供了详实案例,可为线虫在高校遗传学实验或其他相关实验课程如细胞生物学实验、模式生物与发育生物学实验中的应用提供参考。
马小英,赵颖岚,贾方兴,宋亚坤,谢宇聪 . 秀丽隐杆线虫在高校遗传学实验中的应用[J]. 遗传, 2017 , 39(8) : 763 -768 . DOI: 10.16288/j.yczz.17-076
Caenorhabditis elegans is one of the most important model organisms in the study of biology. It is ideal for laboratory teaching due to its short life cycle and low cost. It enriches the teaching content and can motivate students’ interest of learning. In this article, we have shown cased C. elegans for the observation of life cycle and mating, as well as the investigation of single nucleotide polymorphism (SNP) and RNA interfere. In addition, we also discuss the details of the experimental design, basic requirement, preparations and related information. We conclude that C. elegans can be used as the experimental materials for teaching college laboratory courses, such as genetic, cell biology, model biology and developmental biology.
Key words： Caenorhabditis elegans; laboratory teaching; genetic laboratory
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