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2017 , Vol. 39 >Issue 11: 1078 - 1089

DOI: https://doi.org/10.16288/j.yczz.17-130

综述

基因组编辑技术在猪遗传改良中的应用

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  • 1. 中国科学院动物研究所,干细胞与生殖生物学国家重点实验室,北京 100101
    2. 中国科学院大学,北京 100101
    3. 山东荣昌育种股份有限公司,滨州 251900
黄娇娇,博士,研究方向：发育生物学。E-mail: huangjj@ioz.ac.cn|赵建国，博士，研究员，博士生导师，研究方向：发育生物学。E-mail: zhaojg@ioz.ac.cn

收稿日期: 2017-04-07

  修回日期: 2017-05-19

  网络出版日期: 2017-12-20

基金资助

国家自然科学基金项目(81671274, 31402045, 31601008);国家科技重大专项(2016ZX08009003-006-007);中国科学院战略性先导项目(XDA08010304)

收起

Genome editing technologies drive the development of pig genetic improvement

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  • 1. State Key Lab of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
    2. University of Chinese Academy of Sciences, Beijing 100101, China
    3. Company of Rongchang Breeding, Binzhou 251900, China

Received date: 2017-04-07

  Revised date: 2017-05-19

  Online published: 2017-12-20

Supported by

the National Natural Science Foundation of China(81671274, 31402045, 31601008);the National Science and Technology Major Project of the Ministry of Science and Technology of China(2016ZX08009003-006-007);the Strategic Pilot Program of the Chinese Academy of Sciences(XDA08010304)

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摘要

核酸酶介导的基因组编辑技术大幅度提高了编辑真核细胞基因组的能力,给生命科学领域带来了革命性地发展,也给猪的遗传改良带来了全新的契机。本文介绍了基因组编辑技术尤其是CRISPR/Cas9系统的发展以及各种天然存在的和人为改造的Cas9变体的作用特点;汇总了利用基因组编辑技术提高猪生产性能,尤其是改善猪肉品质和抵抗病毒感染的研究进展;分析了目前利用基因组编辑技术推进猪遗传改良所面临的挑战;最后,展望了基于基因组编辑技术的猪遗传改良和品种培育的发展趋势。

关键词： 猪; 基因组编辑; 遗传改良

本文引用格式

黄娇娇, 曹春伟, 郑国民, 赵建国 . 基因组编辑技术在猪遗传改良中的应用[J]. 遗传, 2017 , 39(11) : 1078 -1089 . DOI: 10.16288/j.yczz.17-130

Abstract

Nuclease-mediated genome editing technologies contribute to the rapid advances in life sciences via the ability to edit the genomes within living cells, and present a new era for porcine genetic improvement. In this review, we introduce the development of various genomic editing technologies, particularly CRISPR/Cas9 strategies and characteristics of various naturally occurring and artificially engineered CRISPR enzymes. Also, we summarize progress in pig genetic improvement mediated by genome editing, especially those associated with meat quality traits and anti-virus resistance. We highlight the challenges in the implementation of pig genetic improvement and the prospects of pig genetic breeding based on genome editing technologies.

Key words： pig; genomic editing; genetic improvement

参考文献

[1] Capecchi MR. Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat Rev Genet, 2005, 6(6): 507-512.
[2] Thomas KR, Capecchi MR. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell, 1987, 51(3): 503-512.
[3] Brown JP, Wei WY, Sedivy JM. Bypass of senescence after disruption of p21 CIP1 /WAF1 gene in normal diploid human fibroblasts. Science , 1997, 277(5327): 831-834.
[4] Bibikova M, Carroll D, Segal DJ, Trautman JK, Smith J, Kim YG, Chandrasegaran S. Stimulation of homologous recombination through targeted cleavage by chimeric nucleases. Mol Cell Biol, 2001, 21(1): 289-297.
[5] Bibikova M, Golic M, Golic KG, Carroll D. Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics, 2002, 161(3): 1169-1175.
[6] Christian M, Cermak T, Doyle EL, Schmidt C, Zhang F, Hummel A, Bogdanove AJ, Voytas DF. Targeting DNA double-strand breaks with TAL effector nucleases. Genetics, 2010, 186(2): 757-761.
[7] Makarova KS, Grishin NV, Shabalina SA, Wolf YI, Koonin EV. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct, 2006, 1: 7.
[8] Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 2012, 337(6096): 816-821.
[9] Cong L, Ran FA, Cox D, Lin SL, Barretto R, Habib N, Hsu PD, Wu XB, Jiang WY, Marraffini LA, Zhang F. Multiplex genome engineering using CRISPR/Cas systems. Science, 2013, 339(6121): 819-823.
[10] Zhou JW, Xu QP, Yao J, Yu SM, Cao SZ. CRISPR/Cas9 genome editing technique and its application in site-directed genome modification of animals. Hereditas (Beijing), 2015, 37(10): 1011-1020.
[10] 周金伟, 徐绮嫔, 姚婧, 余树民, 曹随忠. CRISPR/Cas9基因组编辑技术及其在动物基因组定点修饰中的应用. 遗传, 2015, 37(10): 1011-1020.
[11] Xu YY, Yang Q, Ren J. Application of CRISPR/Cas9 mediated genome editing in farm animals. Hereditas (Beijing), 2016, 38(3): 217-226.
[11] 幸宇云, 杨强, 任军. CRISPR/Cas9基因组编辑技术在农业动物中的应用. 遗传, 2016, 38(3): 217-226.
[12] Haft DH, Selengut J, Mongodin EF, Nelson KE. A guild of 45 CRISPR-associated (Cas) protein families and multiple CRISPR/Cas subtypes exist in prokaryotic genomes. PLoS Comput Biol, 2005, 1(6): e60.
[13] Nelson CE, Hakim CH, Ousterout DG, Tha
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