肌动蛋白细胞骨架在小鼠第二生心区祖细胞部署发育中的作用
收稿日期: 2018-10-29
修回日期: 2019-01-18
网络出版日期: 2019-01-29
基金资助
国家自然科学基金项目(31701266)
The role of actin cytoskeleton in regulating the deployment process of mouse cardiac second heart field progenitor cells
Received date: 2018-10-29
Revised date: 2019-01-18
Online published: 2019-01-29
Supported by
[Supported by the National Natural Science Foundation of China ](31701266)
脊椎动物心管起源于早期胚胎的生心中胚层细胞,随后从邻近的咽中胚层和内脏中胚层中逐渐添加第二生心区(second heart field, SHF)祖细胞而延长。SHF细胞向心管贡献受损,使心管不能最大限度地延长,导致一系列的心脏发育缺陷,包括最常见的右心室发育不良与流出道分隔旋转异常等先天性出生缺陷。SHF祖细胞构成非经典顶端-基底极性上皮,并具有顶部单纤毛、动态肌动蛋白(actin)富集基底端的丝状伪足等特点。本文总结了actin细胞骨架在小鼠SHF祖细胞部署发育过程中的研究进展,揭示actin细胞骨架在SHF祖细胞发育特别是SHF细胞向流出道部署过程中的重要性,以期为阐明和理解SHF祖细胞迁移、部署的细胞生物学特性提供一定的理论参考。
刘钟颖,黄霞,李紫怡,杨子豪,袁白银 . 肌动蛋白细胞骨架在小鼠第二生心区祖细胞部署发育中的作用[J]. 遗传, 2019 , 41(2) : 125 -136 . DOI: 10.16288/j.yczz.18-293
The vertebrate heart tube originates from cardiogenic mesodermal cells in the early embryo, and subsequently elongates by progressive addition of second heart field (SHF) progenitor cells from adjacent pharyngeal mesoderm and splanchnic mesoderm. Insufficient addition of SHF cells to the heart tube causes the failure of maximal elongation of the heart tube, which results in a series of developmental defects including the most common congenital birth defects, such as right ventricular dysplasia and outflow tract septation, and alignment anomalies. SHF cells form an atypical, apicobasally polarized epithelium which is characterized by apical monocilia, and dynamic actin-rich basal filopodia. In this review, we summarize recent research progresses of actin cytoskeleton in the deployment process of mouse SHF progenitor cells, and reveal the significance of actin cytoskeleton in SHF development, especially in the deployment of SHF cells to the outflow tract, to provide theoretical reference for elucidating and understanding the biological characteristics of SHF deployment.
Key words： actin; second heart field; cell deployment; mouse
| [1] | Bruneau BG . The developmental genetics of congenital heart disease. Nature, 2008,451(7181):943-948. [DOI] | |||
| [2] | Vincent SD, Mayeuf-Louchart A, Watanabe Y, Brzezinski JA IV, Miyagawa-Tomita S, Kelly RG, Buckingham M . Prdm1 functions in the mesoderm of the second heart field, where it interacts genetically with tbx1, during outflow tract morphogenesis in the mouse embryo. Hum Mol Genet, 2014,23(19):5087-5101. [DOI] | |||
| [3] | Evans SM, Yelon D, Conlon FL, Kirby ML . Myocardial lineage development. Circ Res, 2010,107(12):1428-1444. [DOI] | |||
| [4] | Buckingham M, Meilhac S, Zaffran S . Building the mammalian heart from two sources of myocardial cells. Nat Rev Genet, 2005,6(11):826-835. [DOI] | |||
| [5] | Vincent SD, Buckingham ME . How to make a heart: The origin and regulation of cardiac progenitor cells. Curr Top Dev Biol, 2010,90:1-41. [DOI] | |||
| [6] | Dyer LA, Kirby ML . The role of secondary heart field in cardiac development. Dev Biol, 2009,336(2):137-144. [DOI] | |||
| [7] | Sinha T, Li D, Théveniau-Ruissy M, Hutson MR, Kelly RG, Wang J . Loss of wnt5a disrupts second heart field cell deployment and may contribute to oft malformations in digeorge syndrome. Hum Mol Genet, 2015,24(6):1704-1716. [DOI] | |||
| [8] | Sinha T, Wang B, Evans S, Wynshaw-Boris A, Wang J . Disheveled mediated planar cell polarity signaling is required in the second heart field lineage for outflow tract morphogenesis. Dev Biol, 2012,370(1):135-144. [DOI] | |||
| [9] | Francou A, Saint-Michel E, Mesbah K, Kelly RG . Tbx1 regulates epithelial polarity and dynamic basal filopodia in the second heart field. Development, 2014,141(22):4320-4331. [DOI] | |||
| [10] | Kelly RG . The second heart field. Curr Top Dev Biol, 2012,100:33-65. [DOI] | |||
| [11] | van den Berg G, Abu-Issa R, de Boer BA, Hutson MR, de Boer PA, Soufan AT, Ruijter JM, Kirby ML, van den Hoff MJ, Moorman AF . A caudal proliferating growth center contributes to both poles of the forming heart tube. Circ Res, 2009,104(2):179-188. [DOI] | |||
| [12] | Mesbah K, Harrelson Z, Théveniau-Ruissy M, Papaioannou VE, Kelly RG . Tbx3 is required for outflow tract development. Circ Res, 2008,103(7):743-750. [DOI] | |||
| [13] | Cortes C, Francou A, De Bono C, Kelly RG . Epithelial properties of the second heart field. Circ Res, 2018,122(1):142-154. [DOI] | |||
| [14] | de la Cruz MV, Sánchez Gómez C, Arteaga MM, Argüello C . Experimental study of the development of the truncus and the conus in the chick embryo. J Anat, 1977,123(Pt 3):661-686. [DOI] | |||
| [15] | Viragh S, Challice CE . Origin and differentiation of cardiac muscle cells in the mouse. J Ultrastruct Res, 1973,
/
www.chinagene.cn
版权所有 © 中国科学院遗传与发育生物学研究所 中国遗传学会
地址：北京市朝阳区北辰西路1号院 中国科学院遗传与发育生物学研究所1号楼 邮政编码：100101 联系电话：010-64807669 E-mail：yczz@genetics.ac.cn 
|