环状RNA的产生、研究方法及功能
收稿日期: 2019-04-09
修回日期: 2019-05-07
网络出版日期: 2019-05-07
基金资助
国家自然科学基金项目(31800566);福建省自然科学基金项目(2018J01608);国家重点研发计划项目(2016YFD0600106);国家重点研发计划项目(2018YFD0600101);校国际合作(KXGH17016);福建省科技创新团队项目(118/KLA18069A))
Biogenesis, research methods, and functions of circular RNAs
Received date: 2019-04-09
Revised date: 2019-05-07
Online published: 2019-05-07
Supported by
Supported by the National Natural Science Foundation of China(31800566);Fujian Provincial Natural Science Foundation(2018J01608);The National Key R&D Program of China(2016YFD0600106);The National Key R&D Program of China(2018YFD0600101);The International Science and Technology Cooperation and Exchange Fund(KXGH17016);Fujian Provincial Science and Technology Innovation Team Project(118/KLA18069A))
随着高通量测序技术的发展,环状RNA (circular RNAs, circRNAs)逐渐成为非编码RNA研究领域的热点。本文系统综述了环状RNA侧翼内含子自身互补配对驱动、RNA结合蛋白驱动以及套索驱动这3种环状RNA形成模型,并从高通量文库构建、生物信息学鉴别和常用的实验验证等3个方面对环状RNA的研究方法进行了介绍。同时,本文详细归纳了环状RNA作为microRNA (miRNA)或蛋白的海绵体、调控宿主基因的选择性剪接和表达、翻译成多肽等多种功能。最后通过系统综述植物环状RNA的特征及最新研究进展,为环状RNA在植物学中的进一步研究提供了新的视野。
刘旭庆,高宇帮,赵良真,蔡宇晨,王汇源,苗苗,顾连峰,张航晓 . 环状RNA的产生、研究方法及功能[J]. 遗传, 2019 , 41(6) : 469 -485 . DOI: 10.16288/j.yczz.19-061
The field of circular non-coding RNAs have been gradually attracted wide attention with the developments of high-throughput sequencing. In this review, we systematically summarize three driving models for circRNAs biogenesis: intron-pairing-driven, RNA binding protein-driven and lariat-driven. In addition, we also briefly introduce the current research methods of circRNAs, which include high-throughput library construction methods, identification through bioinformatics and common experimental verification. Here, we also systematically summarize the functions of circRNAs, including microRNA (miRNA) or protein sponges, regulating the alternative splicing (AS) and expression of host genes, and extensive translation. Finally, we provide a systematic characterization and the latest research progress of circRNAs, which provide a new perspective for further studies of circRNAs in plants.
Key words： circular RNAs; back-splicing; alternative splicing; sponge
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