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2019 , Vol. 41 >Issue 9: 883 - 892

DOI: https://doi.org/10.16288/j.yczz.19-122

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现代代谢组学平台建设及相关技术应用

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  • 中国科学院遗传与发育生物学研究所,植物基因组学国家重点实验室,种子创新研究院,北京 100101
张凤霞,博士,工程师,研究方向：代谢组学分析方法及应用。E-mail: zhangfx@genetics.ac.cn

收稿日期: 2019-04-30

  修回日期: 2019-06-30

  网络出版日期: 2019-08-11

基金资助

植物基因组学国家重点实验室资助编号(SKLPG2016A-13);植物基因组学国家重点实验室资助编号(SKLPG2016B-13)

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Current metabolomics platforms: technical composition and applications

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  • State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Innovation Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China

Received date: 2019-04-30

  Revised date: 2019-06-30

  Online published: 2019-08-11

Supported by

Supported by the State Key Laboratory of Plant Genomics of China Nos(SKLPG2016A-13);Supported by the State Key Laboratory of Plant Genomics of China Nos(SKLPG2016B-13)

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摘要

代谢组作为生命科学研究的5个层面(基因组、转录组、蛋白质组、代谢组和表型组)之一越来越受到科研工作者的关注。色谱-质谱联用技术由于其高分离能力、高灵敏度等优点在代谢组学(定性和定量)领域发挥重要的作用,基于色谱-质谱联用技术的代谢组学已成功应用于代谢表型差异研究、基因功能鉴定和转基因生物安全性评价等多个研究方向。本文以中国科学院遗传与发育生物学研究所代谢组学平台为例,详细介绍了现代代谢组学平台色谱-质谱联用仪器的硬件组成,以及不同技术平台在现在系统生物学研究中的具体应用。

关键词： 小分子化合物; 代谢组学平台; 色谱-质谱联用; 系统生物学

本文引用格式

张凤霞,王国栋 . 现代代谢组学平台建设及相关技术应用[J]. 遗传, 2019 , 41(9) : 883 -892 . DOI: 10.16288/j.yczz.19-122

Abstract

Metabolomics (defined as comprehensive small molecule chemical analysis), together with genomics, transcriptomics, proteomics and phenomics, now plays a fundamental role in system biological studies. Chromatography- mass spectrometry machines, which have the characteristics of high resolution and high sensitivity, are widely used for metabolomics analysis, both qualitatively and quantitatively. With the fast development of the chromatography-mass spectrometry technology, metabolomics analysis has been successfully applied in various biological research fields. Here, we introduce the different chromatography-mass spectrum machines used for metabolomics analysis and their applications to various biological issues by mainly using the metabolomics platform in Institute of Genetics and Developmental Biology as a case study.

Key words： small molecule chemicals; metabolomics platform; chromatography-mass spectrometry; system biology

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