RNAi在抗蚊媒病毒感染中的研究进展
收稿日期: 2019-10-15
修回日期: 2019-12-14
网络出版日期: 2020-01-07
基金资助
国家自然科学基金项目(31630011);广东省自然科学基金项目(2017A030313625);广州市科技计划项目(201804020084)
Research progress in RNA interference against the infection of mosquito-borne viruses
Received date: 2019-10-15
Revised date: 2019-12-14
Online published: 2020-01-07
Supported by
National Natural Science Foundation of China(31630011);Natural Science Foundation of Guangdong Province of China(2017A030313625);Science and Technology Planning Project of Guangzhou City(201804020084)
蚊媒病因具有较高的发病率和传播率使其成为全球关注的重要公共卫生问题。蚊虫作为蚊媒病的传播媒介,研究其与蚊媒病毒两者之间的相互作用机制将有助于蚊媒病的防控。蚊虫抵御蚊媒病毒的先天免疫降低和病毒成功逃避蚊虫免疫屏障为病毒在蚊虫体内的持续感染和蚊媒病的暴发流行造成了潜在风险。RNA干扰(RNA interference, RNAi)途径作为蚊虫体内强大的抗病毒防御屏障,通过产生多种小RNA降解病毒RNA,从而达到抑制病毒复制和传播的目的。本文对小干扰RNA (small interfering RNA, siRNA)、微小RNA (microRNA, miRNA)、Piwi蛋白相作用RNA (Piwi-interacting RNA, piRNA)等3种小分子RNA在蚊虫体内发挥抗蚊媒病毒感染的先天免疫机制的相关研究进行了综述,以期为蚊媒病的防控提供理论参考。
魏勇, 何玉兰, 郑学礼 . RNAi在抗蚊媒病毒感染中的研究进展[J]. 遗传, 2020 , 42(2) : 153 -160 . DOI: 10.16288/j.yczz.19-262
Mosquito-borne diseases have become an important public health issue of global concern because of their high incidence and transmission rate. As a vector for mosquito-borne diseases, studying the interaction mechanism between mosquitoes and mosquito-borne viruses will help control mosquito-borne diseases. The impaired innate immunity and immune barriers evasion caused by mosquito-borne viruses in mosquitoes pose a potential risk for the persistent infection of the virus in mosquitoes and the outbreak of mosquito-borne diseases. The RNA interference (RNAi) pathway, as a powerful antiviral defense barrier in mosquitoes, can inhibit viral replication and transmission by producing a variety of small RNAs to degrade viral RNA. In this review, we summarize the related studies on the innate immune mechanism against mosquito- borne virus infection in mosquitoes about small interfering RNA (siRNA), microRNA (miRNA), and Piwi-interacting RNA (piRNA), aiming to provide a theoretical reference for the prevention and control of mosquito-borne diseases.
Key words： Mosquito; Mosquito-borne viruses; siRNA; miRNA; piRNA
| [1] | Gubler DJ . Human arbovirus infections worldwide. Ann N Y Acad Sci, 2001,951:13-24. |
| [2] | Beckham JD, Tyler KL . Arbovirus infections. Continuum (Minneap Minn), 2015,21:1599-1611. |
| [3] | Laureti M, Narayanan D, Rodriguez-Andres J, Fazakerley JK, Kedzierski L . Flavivirus receptors: diversity, identity, and cell entry. Front Immunol, 2018,9:2180. |
| [4] | Lim EXY, Lee WS, Madzokere ET, Herrero LJ . Mosquitoes as suitable vectors for alphaviruses. Viruses, 2018,10(2):E84. |
| [5] | Wuerth JD, Weber F . Phleboviruses and the type I interferon response. Viruses, 2016,8(6):174. |
| [6] | Dash AP, Bhatia R, Sunyoto T, Mourya DT . Emerging and re-emerging arboviral diseases in Southeast Asia. J Vector Borne Dis, 2013,50(2):77-84. |
| [7] | Lowe R, Barcellos C, Brasil P, Cruz OG, Honório NA, Kuper H, Carvalho MS . The Zika virus epidemic in Brazil: From discovery to future implications. Int J Environ Res Public Health, 2018,15(1):96. |
| [8] | Weaver SC, Costa F, Garcia-Blanco MA, Ko AI, Ribeiro GS, Saade G, Shi PY, Vasilakis N . Zika virus: History, emergence, biology, and prospects for control. Antiviral Res, 2016,130:69-80. |
| [9] | Pang T, Mak TK, Gubler DJ . Prevention and control of dengue-the light at the end of the tunnel. Lancet Infect Dis, 2017,17(3):e79-e87. |
| [10] | Xi ZY, Ramirez JL, Dimopoulos G . The Aedes aegypti toll pathway controls dengue virus infection. PLoS Pathog, 2008,4(7):e1000098. |
| [11] | Souza-Neto JA, Sim S, Dimopoulos G . An evolutionary conserved function of the JAK-STAT pathway in anti- dengue defense. Proc Natl Acad Sci USA, 2009,106(42):17841-17846. |
| [12] | Liu XM, Yuan ML . Progress in innate immunity-related genes in insects. Hereditas(Beijing), 2018,40(6):451-466. |
| [12] | 刘小民, 袁明龙 . 昆虫天然免疫相关基因研究进展. 遗传, 2018,40(6):451-466. |
| [13] | Ding SW, Voinnet O . Antiviral immunity directed by small RNAs. Cell, 2007,130(3):413-426. |
| [14] | Nandety RS, Kuo YW, Nouri S, Falk BW . Emerging strategies for RNA interference (RNAi) applications in insects. Bioengineered, 2015,6(1):8-19. |
| [15] | Lee WS, Webster JA, Madzokere ET, Stephenson EB, Herrero LJ . Mosquito antiviral defense mechanisms: a delicate balance between innate immunity and persistent viral infection. Parasit Vectors, 2019,12(1):165. |
| [16] | Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC . Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 1998,391(6669):806-811. |
| [17] | Saleh MC , Tassetto M, van Rij RP, Goic B, Gausson V, Berry B, Jacquier C, Antoniewski C, Andino R. Antiviral immunity in Drosophila requires systemic RNA interference spread. Nature, 2009,458(7236):346-350. |
| [18] | Puglise JM, Estep AS, Becnel JJ . Expression profiles and RNAi silencing of inhibitor of apoptosis transcripts in Aedes, Anopheles, and Culex Mosquitoes (Diptera: Culicidae). J Med Entomol, 2016,53(2):304-314. |
| [19] | Kang S, Hong YS . RNA interference in infectious tropical diseases. Korean J Parasitol, 2008,46(1):1-15. |
| [20] | Gandhi NS, Tekade RK, Chougule MB . Nanocarrier mediated delivery of siRNA/miRNA in combination with chemotherapeutic agents for cancer therapy: current progress and advances. J Control Release, 2014,194:238-256. |
| [21] | Zheng WH, Lin ZQ, Zhuo M, Du HL, Wang XN . Research progress on influenza antiviral small RNAs. Hereditas (Beijing), 2012,34(5):526-532. |
| [21] | 郑维豪, 林志强, 卓敏, 杜红丽, 王小宁 . 抗流感病毒小RNAs研究进展. 遗传, 2012,34(5):526-532. |
| [22] | Galiana-Arnoux D, Dostert C, Schneemann A, Hoffmann JA, Imler JL . Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila. Nat Immunol, 2006,7(6):590-597. |
| [23] | Wang XH, Aliyari R, Li WX, Li HW, Kim K, Carthew R, Atkinson P, Ding SW . RNA interference directs innate immunity against viruses in adult Drosophila. Science, 2006,312(5772):452-454. |
| [24] | Matranga C, Tomari Y, Shin C, Bartel DP, Zamore PD . Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes. Cell, 2005,123(4):607-620. |
| [25] | Miyoshi K, Tsukumo H, Nagami T, Siomi H, Siomi MC . Slicer function of Drosophila argonautes and its involvement in RISC formation. Genes Dev, 2005,19(23):2837-2848. |
| [26] | Li WX, Li H, Lu R, Li F, Dus M, Atkinson P, Brydon EW, Johnson KL, García-Sastre A, Ball LA, Palese P, Ding SW . Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing. Proc Natl Acad Sci USA, 2004,101(5):1350-1355. |
| [27] | Sánchez-Vargas I, Scott JC, Poole-Smith BK, Franz AW, Barbosa-Solomieu V, Wilusz J, Olson KE, Blair CD . Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito's RNA interference pathway. PLoS Pathog, 2009,5(2):e1000299. |
| [28] | Khoo CC, Piper J, Sanchez-Vargas I, Olson KE, Franz AW . The RNA interference pathway affects midgut infection- and escape barriers for Sindbis virus in Aedes aegypti. BMC Microbiol, 2010,10:130. |
| [29] | Basu S, Aryan A, Overcash JM, Samuel GH, Anderson MA, Dahlem TJ, Myles KM, Adelman ZN . Silencing of end-joining repair for efficient site-specific gene insertion after TALEN/CRISPR mutagenesis in Aedes aegypti. Proc Natl Acad Sci USA, 2015,112(13):4038-4043. |
| [30] | Samuel GH, Wiley MR, Badawi A, Adelman ZN, Myles KM . Yellow fever virus capsid protein is a potent suppressor of RNA silencing that binds double-stranded RNA. Proc Natl Acad Sci USA, 2016,113(48):13863-13868. |
| [31] | Keene KM, Foy BD, Sanchez-Vargas I, Beaty BJ, Blair CD, Olson KE . RNA interference acts as a natural antiviral response to O'nyong-nyong virus (Alphavirus; Togaviridae) infection of Anopheles gambiae. Proc Natl Acad Sci USA, 2004,101(49):17240-17245. |
| [32] | Brackney DE, Beane JE, Ebel GD . RNAi targeting of West Nile virus in mosquito midguts promotes virus diversification. PLoS Pathog, 2009,5(7):e1000502. |
| [33] | Myles KM, Morazzani EM, Adelman ZN . Origins of alphavirus-derived small RNAs in mosquitoes. RNA Biol, 2009,6(4):387-391. |
| [34] | Myles KM, Wiley MR, Morazzani EM, Adelman ZN . Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes. Proc Natl Acad Sci USA, 2008,105(50):19938-19943. |
| [35] | Schuster S, Zirkel F , Kurth A, van Cleef KWR, Drosten C, van Rij RP, Junglen S. A unique nodavirus with novel features: mosinovirus expresses two subgenomic RNAs, a capsid gene of unknown origin, and a suppressor of the antiviral RNA interference pathway. J Virol, 2014,88(22):13447-13459. |
| [36] | Szemiel AM, Failloux AB, Elliott RM . Role of Bunyamwera Orthobunyavirus NSs protein in infection of mosquito cells. PLoS Negl Trop Dis, 2012,6(9):e1823. |
| [37] | Kakumani PK, Ponia SS , S RK, Sood V, Chinnappan M, Banerjea AC, Medigeshi GR, Malhotra P, Mukherjee SK, Bhatnagar RK. Role of RNA interference (RNAi) in dengue virus replication and identification of NS4B as an RNAi suppressor. J Virol, 2013,87(16):8870-8883. |
| [38] | Samuel GH, Wiley MR, Badawi A, Adelman ZN, Myles KM . Yellow fever virus capsid protein is a potent suppressor of RNA silencing that binds double-stranded RNA. Proc Natl Acad Sci USA, 2016,113(48):13863-13868. |
| [39] | Jonas S, Izaurralde E . Towards a molecular understanding of microRNA-mediated gene silencing. Nat Rev Genet, 2015,16(7):421-433. |
| [40] | Pedersen IM, Cheng GF, Wieland S, Volinia S, Croce CM, Chisari FV, David M . Interferon modulation of cellular microRNAs as an antiviral mechanism. Nature, 2007,449(7164):919-922. |
| [41] | Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ, Carthew RW . Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell, 2004,117(1):69-81. |
| [42] | Xia MM, Shen XY, Niu CM, Xia J, Sun HY, Zheng Y . MicroRNA regulates Sertoli cell proliferation and adhesion. Hereditas(Beijing), 2018,40(9):724-732. |
| [42] | 夏蒙蒙, 申雪沂, 牛长敏, 夏静, 孙红亚, 郑英 . MicroRNA参与调控睾丸支持细胞的增殖与粘附功能. 遗传, 2018,40(9):724-732. |
| [43] | Trobaugh DW, Klimstra WB . MicroRNA regulation of RNA virus replication and pathogenesis. Trends Mol Med, 2017,23(1):80-93. |
| [44] | Hussain M, Torres S, Schnettler E, Funk A, Grundhoff A, Pijlman GP, Khromykh AA, Asgari S . West Nile virus encodes a microRNA-like small RNA in the 3' untranslated region which up-regulates GATA4 mRNA and facilitates virus replication in mosquito cells. Nucleic Acids Res, 2012,40(5):2210-2223. |
| [45] | Hussain M, Asgari S . MicroRNA-like viral small RNA from Dengue virus 2 autoregulates its replication in mosquito cells. Proc Natl Acad Sci USA, 2014,111(7):2746-2751. |
| [46] | Campbell CL, Harrison T, Hess AM, Ebel GD . MicroRNA levels are modulated in Aedes aegypti after exposure to Dengue-2. Insect Mol Biol, 2014,23(1):132-139. |
| [47] | Slonchak A, Hussain M, Torres S, Asgari S, Khromykh AA . Expression of mosquito microRNA Aae-miR-2940-5p is downregulated in response to West Nile virus infection to restrict viral replication. J Virol, 2014,88(15):8457-8467. |
| [48] | Su JX, Li CX, Zhang YM, Yan T, Zhu XJ, Zhao MH, Xing D, Dong YD, Guo XX, Zhao TY . Identification of microRNAs expressed in the midgut of Aedes albopictus during dengue infection. Parasit Vectors, 2017,10(1):63. |
| [49] | Su JX, Wang G, Li CX, Xing D, Yan T, Zhu XJ, Liu QM, Wu Q, Guo XX, Zhao TY . Screening for differentially expressed miRNAs in Aedes albopictus(Diptera: Culicidae) exposed to DENV-2 and their effect on replication of DENV-2 in C6/36 cells. Parasit Vectors, 2019,12(1):44. |
| [50] | Varjak M, Maringer K, Watson M, Sreenu VB, Fredericks AC, Pondeville E, Donald CL, Sterk J, Kean J, Vazeille M, Failloux AB, Kohl A, Schnettler E . Aedes aegypti Piwi4 is a noncanonical PIWI protein involved in antiviral responses. mSphere, 2017,2(3):e00144-17. |
| [51] | Yin H, Lin HF . An epigenetic activation role of Piwi and a Piwi-associated piRNA in Drosophila melanogaster. Nature, 2007,450(7167):304-308. |
| [52] | Kirino Y, Mourelatos Z . Mouse Piwi-interacting RNAs are 2'-O-methylated at their 3' termini. Nat Struct Mol Biol, 2007,14(4):347-348. |
| [53] | Liu QP, An N, Cen S, Li XY . Molecular mechanisms of genetic transposition inhibition by piRNA. Hereditas (Beijing), 2018,40(6):445-450. |
| [53] | 刘启鹏, 安妮, 岑山, 李晓宇 . piRNA抑制基因转座的分子机制. 遗传, 2018,40(6):445-450. |
| [54] | Siomi MC, Sato K, Pezic D, Aravin AA . PIWI-interacting small RNAs: the vanguard of genome defence. Nat Rev Mol Cell Biol, 2011,12(4):246-258. |
| [55] | Brennecke J, Aravin AA, Stark A, Dus M, Kellis M, Sachidanandam R, Hannon GJ . Discrete small RNA- generating loci as master regulators of transposon activity in Drosophila. Cell, 2007,128(6):1089-1103. |
| [56] | Hayashi R, Schnabl J, Handler D, Mohn F, Ameres SL, Brennecke J . Genetic and mechanistic diversity of piRNA 3'-end formation. Nature, 2016,539(7630):588-592. |
| [57] | Morazzani EM, Wiley MR, Murreddu MG, Adelman ZN, Myles KM . Production of virus-derived ping-pong-dependent piRNA-like small RNAs in the mosquito soma. PLoS Pathog, 2012,8(1):e1002470. |
| [58] | Miesen P , Joosten J, van Rij RP. PIWIs go viral: arbovirus-derived piRNAs in vector mosquitoes. PLoS Pathog, 2016,12(12):e1006017. |
| [59] | Miesen P , Girardi E, van Rij RP. Distinct sets of PIWI proteins produce arbovirus and transposon-derived piRNAs in Aedes aegypti mosquito cells. Nucleic Acids Res, 2015,43(13):6545-6556. |
| [60] | Schnettler E, Donald CL, Human S, Watson M, Siu RW , McFarlane M, Fazakerley JK, Kohl A, Fragkoudis R. Knockdown of piRNA pathway proteins results in enhanced Semliki Forest virus production in mosquito cells. J Gen Virol, 2013,94(Pt 7):1680-1689. |
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