Prime editing引导植物基因组精确编辑新局面
收稿日期: 2020-05-02
修回日期: 2020-05-20
网络出版日期: 2020-05-28
基金资助
国家转基因专项资助编号(2019ZX08010003-001-008);国家转基因专项资助编号(2016ZX08010-002-008)
Prime editing creates a novel dimension of plant precise genome editing
Received date: 2020-05-02
Revised date: 2020-05-20
Online published: 2020-05-28
Supported by
Supported by the Genetically Modified Breeding Major Projects Nos(2019ZX08010003-001-008);Supported by the Genetically Modified Breeding Major Projects Nos(2016ZX08010-002-008)
由于植物细胞内同源重组频率较低、供体传递受限等原因,对植物基因组进行精准编辑十分困难。近期,中国科学院遗传与发育生物学研究所高彩霞团队构建了适用于植物的引导编辑器(plant prime editor, PPE)系统,并在重要作物水稻和小麦中完成了引导编辑。该系统不产生DNA双链断裂,仍可高度准确实现所有可能的12种单碱基替换、多碱基替换及片段缺失插入,从而为植物基因组精确编辑提供了多用途工具。本文介绍了PPE的组成结构和编辑能力,同时也结合其他研究组随后发表的报告综述了植物引导编辑器的优化探索,为合理使用PPEs和继续开展优化工作提供帮助。
秦瑞英, 魏鹏程 . Prime editing引导植物基因组精确编辑新局面[J]. 遗传, 2020 , 42(6) : 519 -523 . DOI: 10.16288/j.yczz.20-125
The precise genome editing has not been well established in plants, largely because of the limited frequency of homology recombination and the delivery barrier of donor templates. Recently, Dr. Caixia Gao’s group from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, developed a series of plant prime editors (PPEs), which mediats the prime editing in the genomes of rice and wheat. The PPE systems are able to generate all 12 kinds of programmable base substitutions, as well desired multiplex nucleotide substitutions and small deletions or insertions without DNA double-strand breaks, thus providing versatile tools for precise plant genome editing. Herein, we introduce the structure and the editing capacity of the PPEs. The attemp on efficiency enhancements of PPEs and other PPEs are also discussed, which may provide a reference for appropriate application of PPEs in plants and also for continuous optimization of the editing tools.
Key words： prime editing; precise editing; CRISPR; genome editing; crop
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