普通小麦相关研究进展在遗传学理论教学中的应用
收稿日期: 2020-04-23
修回日期: 2020-08-08
网络出版日期: 2020-09-01
基金资助
国家自然科学基金青年基金项目资助编号(31300191)
The applications of research progress of common wheat in teaching genetics
Received date: 2020-04-23
Revised date: 2020-08-08
Online published: 2020-09-01
Supported by
Supported by the National Natural Science Foundation for Young Scientists of China No(31300191)
普通小麦(T.aestivum L.)又称异源六倍体小麦,其基因组是由来自3个不同二倍体祖先且亲缘关系较近的基因组(A、B和D)构成。普通小麦的进化历程一直是遗传学教学中阐述物种形成和染色体数目变异机制的经典案例。近年来,伴随着科学技术的快速发展和应用,普通小麦的相关研究在细胞学水平、分子水平、基因组水平均取得了重大突破和进展。本文对普通小麦最新研究成果进行了梳理和总结,将相关前沿科学内容与遗传学各章节的理论教学相结合,并应用于遗传学的理论教学中。这不仅是对经典遗传学教材内容的补充和发展,同时也能够让学生认识到遗传学是一门不断发展的自然科学,在提高学生学习兴趣的同时,实现对遗传学基本内容和前沿科学动态的系统学习。
赵娜, 亓宝, 董芊里, 王晓丽 . 普通小麦相关研究进展在遗传学理论教学中的应用[J]. 遗传, 2020 , 42(9) : 916 -925 . DOI: 10.16288/j.yczz.20-113
Common wheat (T. aestivum L.) is also known as allohexaploid wheat. Its genome is composed of A/B/D sub-genomes from three closely related diploid ancestors. The evolutionary history of common wheat is used as a classic example to illustrate the mechanism of species formation and chromosome number variation in the current genetics class. In recent years, with the rapid development and application of research technologies, there have been many breakthroughs in the study of common wheat, at the cytological, molecular and genomic level. Here, we summarize the latest research achievements on common wheat, and discuss our practice in combining them with the genetics teaching. Our approach is not only a supplement to the current genetics textbooks, but also enables students to realize that genetics is a constantly evolving natural science. We aim to enhance students’ interests in learning, as well as their systematic learning abilities on genetics and related scientific research frontiers.
Key words: genetics; theoretical teaching; common wheat; allopolyploid
| [1] | Huang SX, Sirikhachornkit A, Su XJ, Faris J, Gill B, Haselkorn R, Gornicki P . Genes encoding plastid acetyl- CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat. Proc Natl Acad Sci USA, 2002,99(12):8133-8138. |
| [2] | Jan D . The relationship between the genome of Triticum urartu and the A and B genomes of Triticum aestivum. Can J Genetics Cytol, 1976,18(2):371-377. |
| [3] | Feldman M, Lupton FGH, Miller TE . Wheats. In Evolution of Crop Plants, 2nd ed. London: Longman Scientific, 1995, 184-192. |
| [4] | Marcussen T, Sandve SR, Heier L, Spannagl M, Pfeifer M, Jakobsen KS, Wulff BBH, Steuernagel B, Mayer KFX, Olsen OA . Ancient hybridizations among the ancestral genomes of bread wheat. Science, 2014,345(6194):1250092. |
| [5] | Chester M, Leitch AR, Soltis PS, Soltis DE . Review of the application of modern cytogenetic methods (FISH/GISH) to the study of reticulation (polyploidy/hybridisation). Genes (Basel), 2010,1(2):166-192. |
| [6] | Zhao N, Zhu B, Li MJ, Wang L, Xu LY, Zhang HK, Zheng SS, Qi B, Han FP, Liu B . Extensive and heritable epigenetic remodeling and genetic stability accompany allohexaploidization of wheat. Genetics, 2011,188(3):499-510. |
| [7] | Zhang HK, Bian Y, Gou XW, Dong YZ, Rustgi S, Zhang BJ, Xu CM, Li N, Qi B, Han FP, von Wettstein D, Liu B,. Intrinsic karyotype stability and gene copy number variations may have laid the foundation for tetraploid wheat formation. Proc Natl Acad Sci USA, 2013,110(48):19466-19471. |
| [8] | Graybosch RA . Uneasy Unions: Quality effects of rye chromatin transfers to wheat. J Cereal Sci, 2001,33(1):3-16. |
| [9] | Mago R, Spielmeyer W, Lawrence G, Lagudah E, Ellis J, Pryor A . Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor Appl Genet, 2002,104(8):1327-1324. |
| [10] | Rabinovich SV . Importance of wheat-rye translocations for breeding modern cultivar of Triticum aestivum L. Euphytica, 1998,100:323-340. |
| [11] | Li J, Zhu XG, Wan HS, Wang Q, Tang ZX, Fu SL, Yang ZJ, Yang MY, Yang WY . Identification of the 1RS-7DS.7DL wheat-rye small segment translocation lines. Hereditas (Beijing), 2015,37(6):590-598. |
| [11] | 李俊, 朱欣果, 万洪深, 王琴, 唐宗祥, 符书兰, 杨足君, 杨漫宇, 杨武云 . 1RS-7DS.7DL小麦-黑麦小片段易位系的鉴定. 遗传, 2015,37(6):590-598. |
| [12] | Zhou Y, He ZH, Sui XX, Xia XC, Zhang XK, Zhang GS . Genetic improvement of grain yield and associated traits in the northern China winter wheat region from 1960 to 2000. Crop Sci, 2007,47(1):245-253. |
| [13] | Schlegel R. Current list of wheats with rye and alien introgression. Version 02-14. 2014.http://www.rye-gene-map.de/rye-introgression . |
| [14] | Wei YM, Zheng YL, Zhou RH, Jia JZ . FISH and RFLP were used to detect the rye chromosomes in new multi- spike wheat germplasm 10-A. Bull Bot, 1999,41(7):722-725. |
| [14] | 魏育明, 郑有良, 周荣华, 贾继增 . 应用荧光原位杂交和RFLP标记检测多小穗小麦新种质10-A中的黑麦染色体. 植物学报, 1999,41(7):722-725. |
| [15] | Chen L, Li M, Wang Y, Qiu L, Tang S, Tang ZX, Fu S . Structural variation of chromosomes in wheat-rye 1BL/1RS translocation lines. J Trit Crops, 2015,35(8):1038-1043. |
| [15] | 陈雷, 李萌, 王洋洋, 邱玲, 汤述尧, 唐宗祥, 符书兰 . 小麦-黑麦1BL/1RS易位系中的染色体结构变异. 麦类作物学报, 2015,35(8):1038-1043. |
| [16] | Danilova TV, Friebe B, Gill BS . Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Chromosoma, 2012,121(6):597-611. |
| [17] | Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Sudhakar D, Christou P, Snape JW, Gale MD, Harberd NP . ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature, 1999,400(6741):256-261. |
| [18] | Zhao H . Research and utilization of dwarf genes in wheat. J Hebei Agric Sci, 2004,8(4):96-99. |
| [18] | 赵和 . 小麦矮秆基因研究和利用现状. 河北农业科学, 2004,8(4):96-99. |
| [19] | Yang SJ, Zhang XK, He ZH, Xia XC, Zhou Y . Distrubition of dwarfing genes Rht-B1b and Rht-D1b in Chinese bread wheats detected by STS marker. Sci Agric Sin, 2006,39(8):1680-1688. |
| [19] | 杨松杰, 张晓科, 何中虎, 夏先春, 周阳 . 用STS标记检测矮秆基因Rht-B1b和Rht-D1b在中国小麦中的分布. 中国农业科学, 2006,39(8):1680-1688. |
| [20] | Liu XY, Li S, Wu K, Liu Q, Gao XH, Fu XD . Sustainable crop yields from the coordinated modulation of plant growth and nitrogen metabolism. Chin Sci Bull, 2019,64(25):2633-2640. |
| [21] | Li S, Tian YH, Wu K, Ye YF, Yu JP, Zhang JQ, Liu Q, Hu MY, Li H, Tong YP, Nicholas PH, Fu XD . Modulating plant growth-metabolism coordination for sustainable agriculture. Nature, 2018,560(7720):595-600. |
| [22] | Avni R, Nave M, Barad O, Baruch K, Twardziok SO, Gundlach H, Hale I, Mascher M, Spannagl M, Wiebe K, Jordan KW, Golan G, Deek J, Ben ZB, Ben ZG, Himmelbach A, Maclachlan RP, Sharpe AG, Fritz A, Ben DR, Budak H, Fahima T, Korol A, Faris JD, Hernandez A, Mikel MA, Levy AA, Steffenson B, Maccaferri M, Tuberosa R, Cattivelli L, Faccioli P, Ceriotti A, Kashkush K, Pourkheirandish M, Komatsuda T, Eilam T, Sela H, Sharon A, Ohad N, Chamovitz DA, Mayer KF, Stein N, Ronen G, Peleg Z, Pozniak CJ, Akhunov ED, Distelfeld A . Wild emmer genome architecture and diversity elucidate wheat evolution and domestication. Science, 2017,357(6346):93-97. |
| [23] | Shitsukawa N, Tahira C, Kassai KI, Hirabayashi C, Shimizu T, Takumi S, Mochida K, Kawaura K, Ogihara Y, Murai K . Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat. Plant Cell, 2007,19(6):1723-1737. |
| [24] | Wen SS, Wen N, Pang JS, Langen G, Brew-Appiah RAT, Mejias JH, Osorio C, Yang MM, Gemini R, Moehs CP, Zemetra RS, Kogel KH, Liu B, Wang XZ, von Wettstein D, Rustgi S,. Structural genes of wheat and barley 5-methylcytosine DNA glycosylases and their potential applications for human health. Proc Natl Acad Sci USA, 2012,109(50):20543-20548. |
| [25] | Yu HX, Tian JC . Review of genome B in T.aestivum L. Mol Plant Breed, 2008,6(4):724-732. |
| [25] | 于海霞, 田纪春 . 普通小麦B基因组的研究进展. 分子植物育种, 2008,6(4):724-732. |
| [26] | Dolezel J, Greilhuber J, Suda J . Estimation of nuclear dna content in plants using flow cytometry. Nat Protoc, 2007,2(9):2233-2244. |
| [27] | Brenchley R, Spannagl M, Pfeifer M, Barker GLA, D'Amore R, Allen AM, McKenzie N, Kramer M, Kerhornou A, Bolser D, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Huo NX, Luo MC, Sehgal S, Kianian S, Gill B, Anderson O, Kersey P, Dvorak J, McCombie R, Hall A, Mayer KFX, Edwards KJ, Bevan MW, Hall N. Analysis of the bread wheat genome using whole genome shotgun sequencing. Nature, 2012,491(7426):705-710. |
| [28] | Clavijo BJ, Venturini L, Schudoma C, Accinelli GG, Kaithakottil G, Wright J, Borrill P, Kettleborough G, Heavens D, Chapman H, Lipscombe J, Barker T, Lu FH, McKenzie N, Raats D, Ramirez-Gonzalez RH, Coince A, Peel N, Percival-Alwyn L, Duncan O, Tr?sch J, Yu GT, Bolser DM, Namaati G, Kerhornou A, Spannagl M, Gundlach H, Haberer G, Davey RP, Fosker C, Palma FD, Phillips AL, Millar AH, Kersey PJ, Uauy C, Krasileva KV, Swarbreck D, Bevan MW, Clark MD. An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations. Genome Res, 2017,27(5):885-896. |
| [29] | Zimin AV, Puiu D, Hall R, Kingan S, Clavijo BJ, Salzberg SL . The first near-complete assembly of the hexaploid bread wheat genome, Triticum aestivum. GigaScience, 2017,6(11):1-7. |
| [30] | Ling HQ, Zhao SC, Liu DC, Wang JY, Sun H, Zhang C, Fan HJ, Li D, Dong LL, Tao Y, Gao C, Wu HL, Li YW, Cui Y, Guo XS, Zheng SS, Wang B, Yu K, Liang QS, Yang WL, Lou XY, Chen J, Feng MJ, J B, Zhang XF, Luo GB, Jiang Y, Liu JJ, Wang ZB, Sha YH, Zhang BR, Wu HJ, Tang DZ, Shen QH, Xue PY, Zou SH, Wang XJ, Liu X, Wang FM, Yang YP, An XL, Dong ZY, Zhang KP, Zhang XQ, Luo MC, Dvorak J, Tong YP, Wang J, Yang HM, Li ZS, Wang DW, Zhang AM, Wang J. Draft genome of the wheat A-Genome progenitor Triticum urartu. Nature, 2013,496(7443):87-90. |
| [31] | Ling HQ, Ma B, Shi XL, Liu H, Dong LL, Sun H, Cao YH, Gao Q, Zheng SS, Li Y, Yu Y, Du HL, Qi M, Li Y, Lu HW, Yu H, Cui Y, Wang N, Chen CL, Wu HL, Zhao Y, Zhang JC, Li YW, Zhou WJ, Zhang BR, Hu WJ, Van EMT, Tang JF, Witsenboer HMA, Zhao SC, Li ZS, Zhang AM, Wang DW, Liang CZ . Genome sequence of the progenitor of wheat A subgenome Triticum urartu. Nature, 2018,557(7705):424-428. |
| [32] | Shi XL, He YL, Ling HQ . Progress on wheat A genome illustration and its evolutional analysis. Hereditas(Beijing), 2019,41(9):836-844. |
| [32] | 史晓黎, 何伊琳, 凌宏清 . 小麦A基因组测序与进化研究进展. 遗传, 2019,41(9):836-844. |
| [33] | Luo MC, Gu YQ, Puiu D, Wang H, Twardziok SO, Deal KR, Huo NX, Zhu TT, Wang L, Wang Y, McGuire PE, Liu SY, Long H, Ramasamy RK, Rodriguez JC, Van Sonny L, Yuan LX, Wang ZZ, Xia ZQ, Xiao LC, Anderson OD, Ouyang SH, Liang Y, Zimin AV, Pertea G, Qi P, Bennetzen JL, Dai XT, Dawson MW, Müller HG, Kugler K, Rivarola-Duarte L, Spannagl M, Mayer KFX, Lu FH, Bevan MW, Leroy P, Li PC, You FM, Sun QX, Liu ZY, Lyons E, Wicker T, Salzberg SL, Devos KM, Dvo?ák J. Genome sequence of the progenitor of the wheat D genome Aegilops tauschii. Nature, 2017,551(7681):498-502. |
| [34] | The International Wheat Genome Sequencing Consortium ( IWGSC), Appels R, Kellye E, Nils S, Catherine F, Beat K, Jane R. Shifting the limits in wheat research and breeding using a fully annotated reference genome. Science, 2018, 361(6403): eaar7191. |
| [35] | Sehgal D, Rosyara U, Mondal S, Singh R, Poland J, Dreisigacker S . Incorporating genome-wide association mapping results into genomic prediction models for grain yield and yield stability in CIMMYT spring bread wheat. Front Plant Sci, 2020,11:197. |
| [36] | Liu J, Xu ZB, Fan XL, Zhou Q, Cao J, Wang F, Ji GS, Yang L, Feng B, Wang T . A genome-wide association study of wheat spike related traits in China. Front Plant Sci, 2018,9:1584. |
| [37] | Tsai HY, Janss LL, Andersen JR, Orabi J, Jensen JD, Jahoor A, Jensen J . Genomic prediction and GWAS of yield, quality and disease-related traits in spring barley and winter wheat. Sci Rep, 2020,10(1):3347. |
| [38] | Kumar D, Kumar A, Chhokar V, Gangwar OP, Bhardwaj SC, Sivasamy M, Prasad SVS, Prakasha TL, Khan H, Singh R, Sharma P, Sheoran S, Iquebal MA, Jaiswal S, Angadi UB, Singh G, Rai A, Singh GP, Kumar D and Tiwari R,. Genome-wide association studies in diverse spring wheat panel for stripe, stem, and leaf rust resistance. Front Plant Sci, 2020,11:748. |
| [39] | Singh S, Sehgal D, Kumar S, Arif MAR, Vikram P, Sansaloni CP, Fuentes-Dávila G, Ortiz C . GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm. Sci Rep, 2020,10(1):5999. |
| [40] | Jin JJ, Duan SN, Qi YZ, Yan SH, Li W, Li BY, Xie CJ, Zhen WC, Ma J . Identification of a novel genomic region associated with resistance to Fusarium crown rot in wheat. Theor Appl Genet, 2020,133(7):2063-2073. |
| [41] | Masterson J . Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms. Science, 1994,264(5157):421-424. |
| [42] | Liu QC. Genetics (Third edition). Beijing: Science Press, 2015. |
| [42] | 刘庆昌. 遗传学(第三版). 北京: 科学出版社, 2015. |
/
| 〈 |
|
〉 |