诱导多能干细胞技术及其在疾病研究中的应用 【已撤稿】
收稿日期: 2020-08-12
修回日期: 2020-10-19
网络出版日期: 2020-11-03
基金资助
国家重点基础研究发展规划项目(973计划)资助编号(2012CB967900)
Induced pluripotent stem cell technology and its application in disease research [Retracted]
Received date: 2020-08-12
Revised date: 2020-10-19
Online published: 2020-11-03
Supported by
Supported by the National Basic Research and Development Program of China (973 Program) No(2012CB967900)
自2006年Takahashi和Yamanaka报道生成诱导多能干细胞(induced pluripotent stem cells, iPSCs)以来,多能干细胞领域进入了前所未有的发展状态,在疾病建模、药物发现以及细胞疗法等各方面都发挥重要作用,促进了细胞生物学和再生医学等学科的发展。目前,iPSCs技术已成为研究病理机制的重要工具,利用iPSCs技术筛选的新药物正在研发中,使用iPSCs衍生细胞的临床试验数量也在逐渐增长。iPSCs与基因编辑技术以及3D类器官相结合的最新研究进展促进了iPSCs在疾病研究中的进一步应用。本文介绍了近年来重编程方法的革新,分析了整合病毒载体系统、整合非病毒载体系统、非整合病毒载体系统以及非整合非病毒载体系统四种重编程方法的利弊;同时综述了iPSCs在疾病建模以及临床治疗等方面的最新研究进展,为促进iPSCs各领域的深入研究提供参考。
[本文已经撤稿，撤稿声明]
蔡晨依, 孟飞龙, 饶琳, 刘云玥, 赵小立 . 诱导多能干细胞技术及其在疾病研究中的应用 【已撤稿】[J]. 遗传, 2020 , 42(11) : 1042 -1061 . DOI: 10.16288/j.yczz.20-235
Since Takahashi and Yamanaka reported the generation of induced pluripotent stem cells (iPSCs) in 2006, the field of pluripotent stem cells has entered an unprecedented state of development. It plays an important role in disease modeling, drug discovery and cell therapy, and promotes the development of cell biology and regenerative medicine. At present, iPSC technology has become an important tool for studying of pathological mechanisms. New drugs screened by iPSC technology are being developed, and the number of clinical trials using iPSC-derived cells is gradually increasing. The latest research progress of iPSCs, combined with gene editing technology and 3D organoid methodology, promotes the further applications of iPSCs in disease research. In this review, we introduce the innovation of reprogramming methods in recent years, analyze the advantages and disadvantages of four reprogramming methods: integrated virus vector system, integrated non-viral vector system, non-integrated virus vector system and non-integrated non virus vector system. At the same time, we summarize the latest research progress on iPSCs in disease modeling and clinical treatment strategies, so as to provide a reference for further in-depth research in various fields of iPSCs.
[This paper has been retracted. ]
Key words： iPSCs; reprogramming; disease modeling; cell therapy
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