小鼠耳芥PEBP基因家族全基因组鉴定及表达分析
收稿日期: 2021-08-24
修回日期: 2021-11-15
网络出版日期: 2022-01-06
基金资助
国家自然科学基金项目编号(U1303302);石河子大学国际科技合作项目编号(GJHZ201806);安徽科技学院学科带头人引进人才启动经费项目资助编号(NXYJ202001)
Genome-wide identification and expression analysis of the PEBP genes in Arabidopsis pumila
Received date: 2021-08-24
Revised date: 2021-11-15
Online published: 2022-01-06
Supported by
Supported by the National Natural Science Foundation of China No(U1303302);the International Science and Technology Cooperation Project of Shihezi University No(GJHZ201806);the Talent Introduction Start-up Fund Project of Anhui Science and Technology University(NXYJ202001)
PEBP (phosphatidylethanolamine-binding protein)家族包含保守的磷脂酰乙醇胺结合蛋白结构域,其中FT和TFL1蛋白构成植物成花素-反成花素系统调控植物的开花时间和株型结构被广泛关注。小鼠耳芥(Arabidopsis pumila)是早春短命植物,生长在古尔班通古特沙漠南缘荒漠地带,对环境具有较好的适应性。本研究对小鼠耳芥PEBP基因家族进行全基因组鉴定,发现其基因组包含11个PEBP基因(1个MFT、2个FT、2个TSF、2个TFL1、2个CEN和2个BFT),均由4个外显子与3个内含子组成。共线性分析表明,小鼠耳芥与拟南芥(A. thaliana)、琴叶拟南芥(A. lyrata) PEBP基因间存在11对共线性关系,PEBP家族在小鼠耳芥基因组中发生了明显的扩张,并且ApPEBP基因复制类型为全基因组复制/片段复制。组织表达分析发现ApMFT在种子中高表达,ApFT和ApBFT主要在花和果荚中表达,ApTFL1在茎尖中高表达,但ApCEN在根中高表达。进一步分析了6个ApPEBP基因在4种非生物胁迫下的表达特征,发现在10% PEG6000模拟干旱胁迫下,ApPEBP基因整体上调表达,在低温(4℃)胁迫下整体下调表达。在盐胁迫(250 mmol/L NaCl)下,ApFT1/2下调表达,ApTFL1-1/2先下调表达后上调表达,而ApCEN1/2明显上调表达。高温(40℃)胁迫下,ApFT1/2和ApTFL1-1/2显著下调表达,但ApCEN1/2上调表达。综上,ApCEN1/2在盐、干旱和高温胁迫下均显著上调表达。蛋白互作网络分析表明,ApPEBP主要与开花通路中的相关蛋白及核糖体蛋白互作。推测ApPEBP基因在小鼠耳芥响应荒漠逆境调节生长发育、开花转变过程中起着重要的作用。
李永光, 金玉环, 郭力, 艾昊, 李瑞宁, 黄先忠 . 小鼠耳芥PEBP基因家族全基因组鉴定及表达分析[J]. 遗传, 2022 , 44(1) : 80 -91 . DOI: 10.16288/j.yczz.21-305
The members of the phosphatidylethanolamine-binding protein (PEBP) family harbor a conserved phosphatidylethanolamine-binding protein domain, of which the FT and TFL1 proteins constitute a plant florigen-antiflorigen system regulating flowering time and plant structure. Arabidopsis pumila is an early spring and ephemeral plant, which grows in the southern boundary of the Gulban Tonggut desert and has a good adaptability to the extreme environment. In this study, a genome-wide identification revealed that the A. pumila genome contains 11 PEBP genes (one MFT, two FT, two TSF, two TFL1, two CEN, and two BFT), all of which consist of four exons and three introns. Collinearity analysis showed that there are 11 pairs of collinearity relationships between A. pumila and A. thaliana, and A. lyrata, respectively. The PEBP gene family obviously has expanded in the A. pumila genome, with duplication of ApPEBP genes in the forms of whole genome duplication/segmental duplication. Tissue expression analysis showed that ApMFT was highly expressed in seeds; ApFT and ApBFT were mainly expressed in flowers and siliques; ApTFL1 was highly expressed in shoot apex; and ApCEN was highly expressed in roots. In addition, the expression profiles of ApPEBP genes under four abiotic stresses were also analyzed in this study. The results revealed that ApPEBP genes were generally up-regulated under drought stress (10% PEG6000) and were generally down-regulated under low temperature (4℃) stress. Under salt stress (250 mmol/L NaCl), ApFT1/2 were down-regulated, ApTFL1-1/2 were initially down-regulated and then up-regulated; and ApCEN1/2 were strikingly up-regulated. Under high temperature (40℃) stress, the expressions of ApFT1/2 and ApTFL1-1/2 were significantly down-regulated, but ApCEN1/2 were noticeably up-regulated. Collectively, ApCEN1/2 expressions were significantly up-regulated under salt, drought, and heat stresses. Protein interaction network analysis showed that A. pumila PEBP proteins interacted with proteins in flowering pathways and ribosomal proteins. These results suggested that ApPEBP genes play an important role(s) in the regulation of growth, development, and flowering transition of A. pumila in response to desert adversities.
Key words： ephemeral plant; Arabidopsis pumila; flowering transition; PEBP; adversity
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