欧洲千里光花发育相关基因SvGLOBOSA功能研究
收稿日期: 2022-03-22
修回日期: 2022-05-05
网络出版日期: 2022-06-06
基金资助
浙江省农业科学院合作研发项目资助编号(H20210384)
Functional analysis of flower development related gene SvGLOBOSA from Senecio vulgaris
Received date: 2022-03-22
Revised date: 2022-05-05
Online published: 2022-06-06
Supported by
Supported by Cooperative R & D project of Zhejiang Academy of Agricultural Sciences No(H20210384)
菊科(Asteraceae)植物独特的头状花序具有重要的观赏与研究价值,其花发育分子机制复杂,目前相关报道较少。本研究对欧洲千里光(Senecio vulgaris)转录组数据进行筛选,初步得到MADS-box基因SvGLOBOSA (SvGLO),并对其功能进行了初步分析。通过生物信息学分析基因结构并预测其功能;qRT-PCR分析了SvGLO在野生型欧洲千里光不同组织中的相对表达量;在龙葵(Solanum nigrum)中过表达SvGLO并进行形态学观察;通过组织学染色分析转基因龙葵子房的组织细胞形态变化。结果表明,SvGLO的开放阅读框(open reading frame, ORF)长度为591 bp,编码196个氨基酸;具有典型的MADS-box和K-box结构域且在C末端含有PI基序(motif) EPFSFRVQPMQPNLQE,属于B类MADS-box基因的PI/GLO亚家族。qRT-PCR结果表明,SvGLO在花序组织中高表达,在营养器官中不表达;同时在过表达龙葵中观察萼片获得花瓣的部分特征,心皮转化为雄蕊状器官,果实发育异常。组织学染色分析结果表明,转基因龙葵的子房壁细胞形态和野生型雄蕊的花药壁细胞相似。以上结果表明,欧洲千里光SvGLO可能参与调控花瓣与雄蕊发育。
栾思楠, 刘乐乐, 周佳圆, 努尔阿斯娅·伊马木, 崔敏龙, 朴春兰 . 欧洲千里光花发育相关基因SvGLOBOSA功能研究[J]. 遗传, 2022 , 44(6) : 521 -530 . DOI: 10.16288/j.yczz.22-055
The unique capitulum of Asteraceae has important ornamental and research value. Few studies have described the complex molecular mechanism of flower development. In this study, SvGLOBOSA(SvGLO), the MADS-box gene of Senecio vulgaris, was identified by screening the transcriptome data, and its function was examined. The gene structure was analyzed and its function was predicted through bioinformatics. The relative expression levels in different tissues of wild-type S. vulgaris were analyzed by qRT-PCR. SvGLO was overexpressed in Solanum nigrum and morphological observations were made. Histological staining was used in analyzing the histological changes in the ovary of transgenic S. nigrum. The results showed that the open reading frame of SvGLO was 591 bp long, encoding 196 amino acids. It has typical MADS-box and K-box domains and contains a PI motif at the C-terminal. SvGLO belongs to the PI/GLO subfamily of class B MADS-box genes. qRT-PCR results showed that SvGLO was highly expressed in inflorescence tissues but not in vegetative organs. In SvGLO-overexpressed S. nigrum, the sepals showed some characteristics of petals, carpels transformed into stamen-like organs, and fruit development was abnormal. Histological staining revealed that the morphology of ovary wall cells of transgenic S. nigrum was similar to that of anther wall cells of the stamen of wild-type S. vulgaris. Therefore, SvGLO may be involved in the regulation of petal and stamen development in S. vulgaris.
Key words： S. vulgaris; SvGLO; flower development; functional research
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