生酮饮食激活脂肪酸氧化促进ME区的少突胶质细胞前体细胞增殖
收稿日期: 2023-02-07
修回日期: 2023-03-22
网络出版日期: 2023-04-04
基金资助
保定市科技计划(2141ZF027);国家重点研发计划(2019YFA0801900);国家重点研发计划(2018YFA0801104);国家自然科学基金项目(32070972);国家自然科学基金项目(31771131);国家自然科学基金项目(31800860);国家自然科学基金项目(81891002);国家自然科学基金项目(31921002);中国科学院战略重点研究计划(XDB32020000);中国科学院百人计划(Z181100001518001)
Ketogenic diet promotes the proliferation of oligodendrocyte precursor cells in ME region by activating fatty acid oxidation
Received date: 2023-02-07
Revised date: 2023-03-22
Online published: 2023-04-04
Supported by
Technical Research Program of Baoding City No(2141ZF027);National Key Research & Development Program of China(2019YFA0801900);National Key Research & Development Program of China(2018YFA0801104);National Natural Science Foundation of China(32070972);National Natural Science Foundation of China(31771131);National Natural Science Foundation of China(31800860);National Natural Science Foundation of China(81891002);National Natural Science Foundation of China(31921002);Strategic Priority Research Program of Chinese Academy of Sciences(XDB32020000);Hundred-Talent Program (Chinese Academy of Sciences)(Z181100001518001)
下丘脑正中隆起(mdian eminence，ME)是神经元和少突胶质细胞的可能生态位，营养因素可能通过诱导ME区细胞变化而调控下丘脑功能。为了确定生理条件下休眠的下丘脑干细胞是否存在饮食诱导的可塑性，本研究使用正常饲料、高脂饮食和生酮饮食(一种低碳水、高脂肪的饮食)等不同喂养方式，比较了不同饮食条件下小鼠ME区伸展细胞(tanycytes，TCs)和少突胶质细胞前体细胞(oligodendrocyte precursor cells，OPCs)的增殖情况，首次发现生酮饮食可诱导促进ME区OPCs增殖，阻断脂肪酸氧化通路可抑制生酮饮食诱导的OPCs增殖。本研究初步揭示了饮食诱导对ME区OPCs的影响，为进一步研究ME区OPCs的功能提供了启示。
关键词： 下丘脑; 正中隆起; 少突胶质细胞前体细胞; 生酮饮食
王奔, 李斯, 吴青峰, 穆文辉 . 生酮饮食激活脂肪酸氧化促进ME区的少突胶质细胞前体细胞增殖[J]. 遗传, 2023 , 45(5) : 425 -434 . DOI: 10.16288/j.yczz.23-026
Hypothalamic median eminence (ME) is a potential niche for neurons and oligodendrocytes, and trophic factors may regulate hypothalamic function by inducing cellular changes in the ME region. To determine whether diet-induced plasticity exists in hypothalamic stem cells dormant under physiological conditions, we used a combination of a normal diet, a high-fat diet, and a ketogenic diet (a low-carb, high-fat diet) to compare the proliferation of tanycytes (TCs) and oligodendrocyte precursor cells (OPCs) in the ME area of mice under the different diets. The results showed that the ketogenic diet could induce and promote the proliferation of OPCs in the ME area, and blocking the fatty acid oxidation program could inhibit the proliferation of OPCs induced by a ketogenic diet. This study preliminarily revealed the diet-induced effect on OPCs in the ME region and provided enlightenment for further study on the function of OPCs in the ME region.
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