我国大豆对外依赖度高，加速提高大豆产量是目前亟需解决的问题。利用杂种优势是大幅提高作物产量的有效途径之一，近年来基于隐性核不育基因开发的智能雄性不育系统，为快速利用大豆杂种优势提供了可能。但是，大豆雄性不育基因研究相对滞后。本研究基于课题组大豆花器官转录组数据，筛选到在大豆早期花药中优势表达基因GmFLA22a，编码含有FAS1结构域的成束状阿拉伯半乳糖蛋白，亚细胞定位表明其可能在内质网中发挥功能。利用基因编辑技术获得Gmfla22a突变体，突变体植株在营养生长阶段与对照组相比没有明显差异，但在生殖生长阶段表现为结实率显著降低。Gmfla22a突变体花粉活力和花粉萌发率均无明显异常，组织切片并染色观察发现，突变体植株花药室壁增厚，花粉粒释放延迟、不完全，这可能是导致Gmfla22a结实率降低的原因。综上，本研究初步揭示GmFLA22a可能参与调控大豆雄性育性，为深入揭示其分子功能提供重要遗传材料，同时为大豆杂种优势利用提供基因资源和理论依据。

China has a high dependence on soybean imports, yield increase at a faster rate is an urgent problem that need to be solved at present. The application of heterosis is one of the effective ways to significantly increase crop yield. In recent years, the development of an intelligent male sterility system based on recessive nuclear sterile genes has provided a potential solution for rapidly harnessing the heterosis in soybean. However, research on male sterility genes in soybean has been lagged behind. Based on transcriptome data of soybean floral organs in our research group, a soybean stamen-preferentially expressed gene GmFLA22a was identified. It encodes a fasciclin-like arabinogalactan protein with the FAS1 domain, and subcellular localization studies revealed that it may play roles in the endoplasmic reticulum. Take advantage of the gene editing technology, the Gmfla22a mutant was generated in this study. However, there was a significant reduction in the seed-setting rate in the mutant plants at the reproductive growth stage. The pollen viability and germination rate of Gmfla22a mutant plants showed no apparent abnormalities. Histological staining demonstrated that the release of pollen grains in the mutant plants was delayed and incomplete, which may due to the locule wall thickening in the anther development. This could be the reason of the reduced seed-setting rate in Gmfla22a mutants. In summary, our study has preliminarily revealed that GmFLA22a may be involved in regulating soybean male fertility. It provides crucial genetic materials for further uncovering its molecular function and gene resources and theoretical basis for the utilization of heterosis in soybean.