DNA甲基化修饰是目前研究最透彻的表观修饰，其诱导的抑癌基因沉默与癌症发生关系密切。生物信息学分析是生命科学、医学等领域重要的研究手段。为了使学生了解生物信息学分析在癌症表观遗传学研究中的应用，并改变遗传学实验教学中学生被动参与实验的现状，本文建立了融入生物信息学分析的综合性探究型表观遗传实验。本实验遵循以学生为中心、以培养科研能力为导向的原则，引导学生从生物信息学分析和文献调研出发，筛选肺腺癌中因DNA高甲基化导致表达沉默的潜在基因，自主制定实验方案，开展实时荧光定量PCR和甲基化特异性PCR实验探究DNA甲基化是否导致目的基因的表达沉默，并确定调控目的基因甲基化的DNA甲基转移酶。采用多元化评价方式，加大实验过程考核占比，综合评估学生的能力。本实验具有跨学科、综合性和探究性的特点，有助于培养学生的科研思维和实践能力。

As the most thoroughly studied epigenetic modification, DNA methylation-induced silencing of suppressor genes is closely related with the development of cancers. Bioinformatic analysis is an important research tool in the fields of life sciences, medicine, and so on. To introduce the application of bioinformatic analysis in cancer epigenetic research to students and to change the current situation that students usually passively perform experiments during the experimental teaching of genetics, we established a comprehensive and exploratory epigenetic experiment which was integrated with bioinformatic analysis. The implement of this experiment followed the principles of centering on students and cultivating scientific research capabilities. Students selected the genes that were potentially silenced by high DNA methylation in lung adenocarcinoma through bioinformatic analysis and literature review under the guidance of teachers and formulated experimental scheme by their own. They then conducted real-time PCR and methylation-specific PCR to explore whether DNA methylation caused the expression silencing of genes of interest and to identify the DNA methyltransferase responsible for the methylation of genes of interest. To comprehensively assess the capability of students, we designed diversified evaluation methods which intensify the assessment of experiment process. This experiment has the characteristics of interdisciplinarity, comprehensiveness, and inquiry, which can help to cultivate the scientific thinking and practical ability of students.