基于转录组学分析秀丽线虫对乙酸钙不动杆菌的摄食偏好行为
刘姣姣和卢心河并列第一作者。
收稿日期: 2024-12-02
修回日期: 2025-01-15
网络出版日期: 2025-03-13
基金资助
海南省重点研发项目(ZDYF2022SHFZ321);海南省自然科学基金项目(823QN230)
Analysis of food preference behavior of C. elegans to Acinetobacter calcoaceticus based on transcriptomics
Received date: 2024-12-02
Revised date: 2025-01-15
Online published: 2025-03-13
Supported by
Hainan Province Key Research and Development Project(ZDYF2022SHFZ321);Natural Science Foundation of Hainan Province(823QN230)
秀丽线虫(C. elegans)是生物学研究的重要模式生物之一，在自然环境中选择细菌食物来源时表现出明显的偏好，然而食物选择这一与进化优势息息相关的生理现象背后的机制并不完全清楚。乙酸钙不动杆菌(Acinetobacter calcoaceticus，Ac)是一种广泛分布于潮湿环境中的革兰氏阴性杆菌。本研究通过比较秀丽线虫在两种细菌食源下的食物偏好性、咽泵频率、体脂含量、寿命长短以及基因表达差异，揭示了秀丽线虫摄食Ac引起脂质代谢变化以及摄食偏好行为，并利用转录组学方法分析了秀丽线虫摄食大肠杆菌OP50和Ac后的基因表达差异，为理解秀丽线虫与微生物互作机制提供了新的视角。
刘姣姣, 卢心河, 殷红彦, 周海龙, 李汉增, 徐顺清 . 基于转录组学分析秀丽线虫对乙酸钙不动杆菌的摄食偏好行为[J]. 遗传, 2025 , 47(7) : 786 -796 . DOI: 10.16288/j.yczz.24-364
C. elegans is one of the most important model organisms in biological research, exhibiting distinct preferences for bacterial food sources in natural environments. Acinetobacter calcoaceticus(Ac) is a widely distributed Gram-negative bacterium commonly found in moist habitats. This study investigates the feeding preferences, pharyngeal pumping rates, body fat content, lifespan, and gene expression differences of C. elegans when fed different bacterial food sources. The results reveal that feeding on Ac induces lipid metabolism changes, leading to altered feeding preferences. Transcriptomic analysis was performed to compare the gene expression profiles of C. elegans fed on Escherichia coli OP50 and Ac, offers a novel perspective on the mechanisms underlying C. elegans microbe interactions.
Key words： C. elegans; Acinetobacter calcoaceticus; food preferences; transcriptome
| [1] | Zečić A, Dhondt I, Braeckman BP. The nutritional requirements of Caenorhabditis elegans. Genes Nutr, 2019, 14: 15. |
| [2] | Zhang JY, Holdorf AD, Walhout AJ. C. elegans and its bacterial diet as a model for systems-level understanding of host-microbiota interactions. Curr Opin Biotechnol, 2017, 46: 74-80. |
| [3] | Schulenburg H, Félix MA. The natural biotic environment of Caenorhabditis elegans. Genetics, 2017, 206(1): 55-86. |
| [4] | Abada EA, Sung H, Dwivedi M, Park BJ, Lee SK, Ahnn J. C. elegans behavior of preference choice on bacterial food. Mol Cells, 2009, 28(3): 209-213. |
| [5] | Freyth K, Janowitz T, Nunes F, Voss M, Heinick A, Bertaux J, Scheu S, Paul RJ. Reproductive fitness and dietary choice behavior of the genetic model organism Caenorhabditis elegans under semi-natural conditions. Mol Cells, 2010, 30(4): 347-353. |
| [6] | Kim DH, Flavell SW. Host-microbe interactions and the behavior of Caenorhabditis elegans. J Neurogenet, 2020, 34(3-4): 500-509. |
| [7] | Shtonda BB, Avery L. Dietary choice behavior in Caenorhabditis elegans. J Exp Biol, 2006, 209(Pt 1): 89-102. |
| [8] | Zhang Y, Lu H, Bargmann CI. Pathogenic bacteria induce aversive olfactory learning in Caenorhabditis elegans. Nature, 2005, 438(7065): 179-184. |
| [9] | Haçariz O, Viau C, Karimian F, Xia JG. The symbiotic relationship between Caenorhabditis elegans and members of its microbiome contributes to worm fitness and lifespan extension. BMC Genomics, 2021, 22(1): 364. |
| [10] | Midha A, Schlosser J, Hartmann S. Reciprocal interactions between nematodes and their microbial environments. Front Cell Infect Microbiol, 2017, 7: 144. |
| [11] | Benoit T, Sajjad D, Cloutier M, Lapen DR, Craiovan E, Sykes EME, Kumar A, Khan IUH. Acinetobacter calcoaceticus-baumannii complex prevalence, spatial- temporal distribution, and contamination sources in Canadian aquatic environments. Microbiol Spectr, 2024, 12(10): e0150924. |
| [12] | Yuan WQ. Clinical distribution and drug resistance of 417 strains of Acinetobacter calcoaceticus. Chin Pract Med, 2013, 8(16): 154-155. |
| 袁文清. 417株醋酸钙不动杆菌的临床分布及耐药性研究. 中国实用医药, 2013, 8(16): 154-155. | |
| [13] | Li Y, Wang XY, Luo H, Yang MD, Li J, Li LF, Zhao JP, Bi J, Chen B, Wang RG. Application of Caenorhabditis elegans in pathogenicity evaluation of Acinetobacter calcoaceticus. J Environ Health, 2020, 37(3): 228-232. |
| 李煜, 王雪岩, 罗环, 杨梦迪, 梁婧, 李隆飞, 赵金萍, 毕洁, 陈斌, 王如刚. 秀丽隐杆线虫在乙酸钙不动杆菌致病性评价中的应用. 环境与健康杂志, 2020, 37(3): 228-232. | |
| [14] | Butcher RA, Ragains JR, Li WQ, Ruvkun G, Clardy J, Mak HY. Biosynthesis of the Caenorhabditis elegans dauer pheromone. Proc Natl Acad Sci USA, 2009, 106(6): 1875-1879. |
| [15] | Lee D, Fox BW, Palomino DF, Panda O, Tenjo FJ, Koury EJ, Evans KS, Stevens L, Rodrigues PR, Kolodziej AR, Schroeder FC, Andersen EC. Natural genetic variation in the pheromone production of C. elegans. Proc Natl Acad Sci USA, 2023, 120(26): e2221150120. |
| [16] | Ren HS, Yin K, Lu XH, Liu JJ, Li DD, Liu ZJ, Zhou HL, Xu SQ, Li HZ. Synergy between nanoplastics and benzo[a]pyrene promotes senescence by aggravating ferroptosis and impairing mitochondria integrity in Caenorhabditis elegans. Sci Total Environ, 2024, 946: 174418. |
| [17] | Wan QL, Meng X, Wang CY, Dai WY, Luo ZH, Yin ZN, Ju ZY, Fu XD, Yang J, Ye QS, Zhang ZH, Zhou QH. Histone H3K4me3 modification is a transgenerational epigenetic signal for lipid metabolism in Caenorhabditis elegans. Nat Commun, 2022, 13(1): 768. |
| [18] | Guo XL, Zhang HL, Zheng XP, Zhou QJ, Yang Y, Chen XQ, Du AF. Structural and functional characterization of a novel gene, Hc-daf-22, from the strongylid nematode Haemonchus contortus. Parasit Vectors, 2016, 9(1): 422. |
| [19] | Yu GC. Thirteen years of clusterProfiler. Innovation (Camb), 2024, 5(6): 100722. |
| [20] | Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol, 2014, 15(12): 550. |
| [21] | Xu SB, Hu EQ, Cai YT, Xie ZJ, Luo X, Zhan L, Tang WL, Wang QW, Liu BD, Wang R, Xie WQ, Wu TZ, Xie LW, Yu GC. Using clusterProfiler to characterize multiomics data. Nat Protoc, 2024, 19(11): 3292-3320. |
| [22] | Ginestet C. ggplot2: C.elegant graphics for data analysis. J R Stat Soc A Stat, 2011, 174(1): 245-246. |
| [23] | Chan JP, Wright JR, Wong HT, Ardasheva A, Brumbaugh J, McLimans C, Lamendella R. Using bacterial transcriptomics to investigate targets of host-bacterial interactions in Caenorhabditis elegans. Sci Rep, 2019, 9(1): 5545. |
| [24] | Li Y, Ding WQ, Li CY, Liu Y. HLH-11 modulates lipid metabolism in response to nutrient availability. Nat Commun, 2020, 11(1): 5959. |
| [25] | Zhang YR, Zou XJ, Ding YH, Wang HZ, Wu XY, Liang B. Comparative genomics and functional study of lipid metabolic genes in Caenorhabditis elegans. BMC Genomics, 2013, 14: 164. |
| [26] | Yu L, Yan XM, Ye CL, Zhao HY, Chen XY, Hu F, Li HX. Bacterial respiration and growth rates affect the feeding preferences, brood size and lifespan of Caenorhabditis elegans. PLoS One, 2015, 10(7): e0134401. |
| [27] | Khan F, Jain S, Oloketuyi SF. Bacteria and bacterial products: foe and friends to Caenorhabditis elegans. Microbiol Res, 2018, 215: 102-113. |
| [28] | White JQ, Nicholas TJ, Gritton J, Truong L, Davidson ER, Jorgensen EM. The sensory circuitry for sexual attraction in C. elegans males. Curr Biol, 2007, 17(21): 1847-1857. |
| [29] | Yi JX, Li R, Chen QL, Luo HW, Li DL, Wang F. Bx-Daf-22 in bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae): cloning and expression. Chin Agric Sci Bull, 2018, 34(30): 71-75. |
| 易家娴, 李仁, 陈俏丽, 罗洪巍, 李丹蕾, 王峰. 松材线虫Bx-Daf-22基因鉴定及表达. 中国农学通报, 2018, 34(30): 71-75. |
/
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:
