义乌小鲵肢体再生及其分子机制

陈昊, 董瑞, 王岩峰, 谢净倍, 张招旭, 郑荣泉

doi:10.16288/j.yczz.24-326

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2025 , Vol. 47 >Issue 9: 1032 - 1041

DOI: https://doi.org/10.16288/j.yczz.24-326

研究报告

义乌小鲵肢体再生及其分子机制

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1.浙江师范大学，野生动物生物技术与保护利用省重点实验室，金华 321004
2.浙江省永康市自然资源和规划局，永康 321300
3.浙江师范大学行知学院，金华 321000

陈昊，硕士研究生，专业方向：动物学研究。E-mail: 898148121@qq.com

郑荣泉，教授，研究方向：动物保护生物学。E-mail: zhengrq@zjnu.cn

收稿日期: 2024-11-11

修回日期: 2025-01-17

网络出版日期: 2025-05-07

基金资助

国家自然科学基金项目(32370454);浙江省重点研发项目(2021C02044);金华市生态环境局义乌分局珍稀濒危野生动物(义乌小鲵)抢救保护行动项目(ZXZJZC2022331GK);永康市野生动物资源本底调查项目资助

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The molecular mechanism of limb regeneration of Hynobius yiwuensis

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1. Provincial Key Laboratory of Wildlife Biotechnology Protection and Utilization, Zhejiang Normal University, Jinhua 321004, China
2. Zhejiang Province Yongkang Municipal Natural Resources and Planning Bureau, Yongkang 321300, China
3. Xingzhi College, Zhejiang Normal University, Jinhua 321000, China

Received date: 2024-11-11

Revised date: 2025-01-17

Online published: 2025-05-07

Supported by

National Natural Science Foundation of China(32370454);Key R&D Program of Zhejiang Province(2021C02044);Rescue and Conservation Project for Rare and Endangered Wildlife (Hynobius yiwuensis) Funded by the Yiwu Branch of the Jinhua Ecological Environment Bureau(ZXZJZC2022331GK);Wildlife Resource Background Survey Project of Yongkang City

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摘要

义乌小鲵(Hynobius yiwuensis)是中国特有的易危小鲵科动物，仅分布于浙江省的部分丘陵山地。本研究采用体式显微镜观察了义乌小鲵断肢再生的过程，并利用转录组测序技术，分析再生过程差异表达基因。结果表明：义乌小鲵肢体具有较强的再生能力，再生过程可分为创伤愈合、组织溶解与去分化、再生芽基形成、形态发生及再分化4个阶段。转录组测序分析表明，肢体再生过程中存在大量差异表达的基因，且其表达模式随肢体切除后的时间推移发生动态变化。通过筛选肢体再生过程中的差异表达基因，发现IL10参与细胞免疫及炎症反应，TGFβ3促进早期肌肉组织再生，而MMPs家族相关基因在组织重塑过程中发挥重要作用。对10个差异基因进行qRT-PCR验证，结果证明转录组测序结果可靠。本研究初步推测义乌小鲵可能通过Wnt/β-catenin、TGFβ、BMP等信号通路调控肢体再生过程，并促进无瘢痕组织修复。

关键词： 义乌小鲵; 肢体再生; 转录组; 差异表达基因

本文引用格式

陈昊, 董瑞, 王岩峰, 谢净倍, 张招旭, 郑荣泉 . 义乌小鲵肢体再生及其分子机制[J]. 遗传, 2025 , 47(9) : 1032 -1041 . DOI: 10.16288/j.yczz.24-326

Abstract

Hynobius yiwuensis is a vulnerable species endemic to China, restricted to specific hilly regions in Zhejiang Province. This study employed stereomicroscopy to examine the limb regeneration process in H. yiwuensis and utilized transcriptome sequencing to analyze differentially expressed genes. The results indicate that H. yiwuensis possesses strong regenerative capabilities, with the regeneration process comprising four stages: wound healing, tissue dissolution and dedifferentiation, blastema formation, and morphogenesis followed by redifferentiation. Transcriptome analysis identified numerous differentially expressed genes during limb regeneration, exhibiting distinct expression patterns at various time points post-amputation. Key differentially expressed genes were identified, including IL10, associated with cellular immunity and inflammation; TGFβ3, involved in early muscle tissue regeneration; and MMPs, implicated in tissue remodeling. qRT-PCR validation of selected differentially expressed genes confirmed the reliability of the transcriptome sequencing data. Preliminary findings suggest that H. yiwuensis regulates limb regeneration and promotes scar-free tissue repair through signaling pathways such as Wnt/β-catenin, TGFβ, and BMP.

Key words： Hynobius yiwuensis; limb regeneration; transcriptome; differentially expressed genes

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