鱼类的性腺分化与配子发生受到多种因子的调控。蛋白磷酸酶(protein phosphatase，PP)具有催化生物体中蛋白去磷酸化的功能。蛋白磷酸酶6 (protein phosphatase type 6，PP6)作为蛋白磷酸酶家族的一员，在性腺发育及配子发生过程中发挥重要功能。ppp6r3编码蛋白磷酸酶6调节亚基3，其在性腺分化和配子发生方面的作用还不清楚。本研究通过CRISPR/Cas9技术构建了两个斑马鱼ppp6r3突变品系，发现ppp6r3缺失后导致斑马鱼仅发育为雄鱼，且该突变体不能使野生型卵子受精。进一步研究发现，ppp6r3突变体精巢中精母细胞向成熟精子转换失败，精子发生受阻。综上所述，本研究构建了ppp6r3突变的斑马鱼模型，表现出性腺分化和配子发生的缺陷，为后续研究Ppp6r3调控生殖细胞增殖、分化及性别决定的机制提供了模型和参考。

The gonad differentiation and gametogenesis of fish is regulated by various factors. Protein phosphatase (PP) have the function of catalyzing the dephosphorylation of proteins in organisms. As a member of the protein phosphatase family, protein phosphatase type 6 (PP6) plays an important role in gonadal development and gametogenesis. The role of ppp6r3, which encodes the regulatory subunit 3 of protein phosphatase 6, in gonadal differentiation and gametogenesis is still unclear. In this study, two zebrafish ppp6r3 mutant lines were constructed using CRISPR/Cas9 technology. It was found that the absence of ppp6r3 leads to the development of only male zebrafish, and these mutants are incapable of fertilizing wild-type eggs. Further investigations revealed that in the testes of ppp6r3 mutants, the transition of spermatocytes to mature sperm was blocked, disrupting spermatogenesis. In summary, this study established a ppp6r3 mutant model, exhibiting defects in gonadal differentiation and gametogenesis, which provides a new model for further research on the mechanisms by which Ppp6r3 regulates germ cell proliferation, differentiation, and sex determination.