pnr与fred协同介导果蝇背板中部SOP细胞发生的双重调控
收稿日期: 2025-03-24
修回日期: 2025-05-07
网络出版日期: 2025-05-30
基金资助
国家自然科学基金项目(32270528);湖南省研究生科研创新项目(CX20231055)
pnr cooperate with fred to regulate SOP cell fate both positively and negatively in the medial notum of Drosophila
Received date: 2025-03-24
Revised date: 2025-05-07
Online published: 2025-05-30
Supported by
National Natural Science Foundation of China(32270528);Hunan Provincial Graduate Research Innovation Project(CX20231055)
根据“预模式假说(Prepattern Hypothesis)”，黑腹果蝇(Drosophila melanogaster)背部感觉器官前体(sensory organ precursors，SOP)的发育依赖于预模式基因、原神经基因和神经源性基因的依次调控。大多数情况下，单一调控基因的功能失常引起的异位SOP形成，或表现为空间不连续，或仅限于原神经簇内。而fred基因的敲低会造成一种异位SOP发生的新型表型，这些异位SOP在空间上是连续的，且不局限于原神经簇区域。本研究发现，由敲减fred所诱导的异位SOP形成不依赖于原神经簇，表明几乎所有翅成虫盘细胞都具有潜在的神经发生潜力。并且，本研究还发现预模式基因pannier(pnr)与fred协同作用，以两种截然不同的方式调控SOP细胞的命运：一方面，pnr和fred在背板中部对于内源性SOP的形成是必需的；但另一方面，它们在原神经簇外协同抑制SOP发生。本研究结果打破了“预模式假说”中SOP形成依赖于原神经簇形成的论断，为该假说提供了一项重要补充。
宋凯月, 张翼飞, 张瑶, 龙淑婷, 彭建军, 孙远东, 崔小娟, 潘本山, 柴语心, 王晓霞, 陈家慧 . pnr与fred协同介导果蝇背板中部SOP细胞发生的双重调控[J]. 遗传, 2026 , 48(1) : 87 -101 . DOI: 10.16288/j.yczz.25-080
According to the “Prepattern Hypothesis”, the development of dorsal sensory organ precursors (SOP) in Drosophila melanogaster depends on the sequential regulation of prepattern genes, proneural genes, and neurogenic genes. In most cases, ectopic SOP formation caused by dysfunction of a single regulatory gene either exhibits spatial discontinuity or is restricted to proneural clusters. However, knockdown of the fred gene induces a novel phenotype of ectopic SOP that are spatially continuous and not confined to proneural cluster regions. This study reveals that fred knockdown-induced ectopic SOP formation is independent of proneural clusters, suggesting that nearly all wing imaginal disc cells possess neurogenic potential. Furthermore, we demonstrate that the prepattern gene pannier (pnr) cooperates with fred to regulate SOP cell fate through two distinct mechanisms: (1) pnr and fred are essential for endogenous SOP formation in the medial notum, while (2) they synergistically suppress SOP initiation outside proneural clusters. These findings challenge the canonical assertion in the “Prepattern Hypothesis” that SOP formation strictly relies on proneural cluster formation, thereby providing a critical extension to the hypothesis.
Key words： fred; neurogenic potential; sense organ precursors; pnr; cell fate determination
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