基于秀丽线虫构建多维度衰老实验的教学设计
收稿日期: 2025-11-27
修回日期: 2025-12-24
网络出版日期: 2026-02-11
基金资助
国家重点研发计划(2022YFA0806400);国家自然科学基金项目(32271215)
Teaching design of a multi-dimensional aging experiment using the Caenorhabditis elegans model
Received date: 2025-11-27
Revised date: 2025-12-24
Online published: 2026-02-11
Supported by
National Key Research and Development Program of China(2022YFA0806400);National Natural Science Foundation of China(32271215)
秀丽线虫(Caenorhabditis elegans)是生命科学领域的经典模式生物，具有易培养、生命周期短和遗传背景清晰等优势，已被广泛应用于发育调控、衰老、遗传及应激反应等研究中。本文基于递进式学习理念与探究式教学思路，以遗传学模式生物秀丽线虫为教学材料，针对衰老这一复杂的生理过程设计了包含形态观察、游泳行为分析、显微结构识别和肠屏障检测4个模块的实验教学体系。教学过程中融入显微成像、行为学定量分析等技术，培养学生的实验技能、逻辑推理与科学表达能力。教学实践结果表明，该实验教学方案具有周期短、成本低、安全性高和实验结果清晰直观等教学优势，能有效激发学生的科研兴趣，为生命科学本科教学改革与遗传学模式生物的教学应用提供参考。
腾丽娟, 王嘉乐, 王会, 熊悦然, 杨亚军, 张景彦 . 基于秀丽线虫构建多维度衰老实验的教学设计[J]. 遗传, 2026 , 48(4) : 432 -439 . DOI: 10.16288/j.yczz.25-312
Caenorhabditis elegans (C. elegans), a classical model organism in the field of life sciences, possesses advantages including easy cultivation, a short life cycle, and a well-defined genetic background. It has been widely used in studies of developmental regulation, aging, genetics, and stress responses. To improve undergraduates’ research literacy and experimental competence, we designed an experimental teaching system centered on C. elegans, which integrates both scientific and practical feasibility guided by the principles of “progressive learning” and inquiry-based teaching. Our system consists of four experimental modules: morphological observation, behavioral analysis, microscopic structure identification, and intestinal barrier assessment. Advanced techniques such as microscopic imaging and quantitative behavioral analysis are incorporated into the teaching process to cultivate students’ experimental skills and scientific communication abilities. Teaching practice has demonstrated that our approach offers multiple advantages, including a short experimental cycle, low cost, high safety, and clear, intuitive results, which effectively stimulate students’ interest in scientific research and foster the development of scientific thinking. This study provides valuable insights for teaching reform in undergraduate life science courses and for the broader application of genetic model organisms.
Key words： Caenorhabditis elegans; aging; genetic teaching; model organism; teaching reform
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