broad在果蝇成虫肠道干细胞中的作用及其机制研究
收稿日期: 2026-01-14
修回日期: 2026-02-16
网络出版日期: 2026-04-24
基金资助
山东省自然科学基金项目(ZR2023MC207)
Role of broad in intestinal stem cells of adult Drosophila
Received date: 2026-01-14
Revised date: 2026-02-16
Online published: 2026-04-24
Supported by
Shandong Provincial Natural Science Foundation of China(ZR2023MC207)
蜕皮激素信号通路(ecdysone signaling)通过促进干细胞增殖与分化，在成年黑腹果蝇(Drosophila melanogaster)肠道稳态维持中具有重要作用。然而，其下游靶基因broad(br)在该过程中的功能尚不明确。本研究证明，br基因对肠道干细胞(intestinal stem cells，ISCs)的维持及肠道损伤修复过程不可或缺。克隆分析结果显示，br同时调控ISCs的增殖与分化。敲低br表达会严重阻碍干细胞向肠上皮细胞(enterocytes，ECs)的分化，而过表达br则会促进其分化。进一步分析发现，敲低br表达会导致ISCs中Jak/STAT信号通路活性降低，而上调Jak/STAT或EGFR信号通路的活性都可以显著挽救br敲低导致的表型。这些结果表明，在成年果蝇中，br可能通过Jak/STAT和EGFR信号通路调控ISCs的增殖与分化。本研究揭示了br基因在果蝇成虫ISCs中的作用，并初步探讨了其机制。
吉长皓, 王暖, 王欣阳, 张美珠, 孙锦 . broad在果蝇成虫肠道干细胞中的作用及其机制研究[J]. 遗传, 2026 , 48(6) : 601 -613 . DOI: 10.16288/j.yczz.26-004
Ecdysone signaling is necessary for maintaining intestinal homeostasis in adult Drosophila melanogaster by promoting stem cell proliferation and differentiation. However, the role of its downstream target broad (br) in this process remains largely unclear. Here, this study demonstrates that br is required for the maintenance of intestinal stem cells (ISCs) and intestinal injury repair. Clonal analysis shows that br regulates both the proliferation and differentiation of ISCs. Knockdown of br severely impairs the differentiation of stem cells into enterocytes (ECs), whereas overexpression of br promotes ECs differentiation. Further analysis reveals that br knockdown reduces Jak/STAT signaling activity in ISCs. Moreover, upregulating the activity of Jak/STAT or EGFR signaling significantly rescues the br knockdown phenotype. These results suggest that br may regulate the proliferation and differentiation of ISCs through Jak/STAT and EGFR signaling in adult Drosophila. This study highlights the critical role of br in adult Drosophila ISCs and provides a preliminary exploration of its underlying mechanism.
Key words： broad; Drosophila melanogaster; intestinal stem cells; Jak/STAT; EGFR
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