丝盘虫（Trichoplax adhaerens）是现存最古老的多细胞动物之一，也是研究多细胞生命起源、细胞类型演化及多细胞协同互作的重要演化节点模型。然而，在实验室培养条件下，丝盘虫种群目前仍主要依赖人工传代维持，缺乏稳定的中长期保存策略，从而限制了其进一步研究与资源共享。本文以 T. adhaerens Grell strain/H1 为材料，分析了不同温度条件下丝盘虫的生长发育、行为变化及基因表达模式，探讨其低温保存的可行性。结果表明，与常规培养条件相比，16°C低温培养能够显著降低丝盘虫的运动活性和生长速率，同时维持其基本生理活动。行为学与转录组分析进一步表明，丝盘虫可通过下调糖类和脂类代谢相关过程，延缓生长发育，以适应低温环境变化。在恢复至常温培养后，经低温处理的丝盘虫能够迅速恢复正常的生长状态。基于上述结果，本研究进一步优化了丝盘虫的低温保存策略，实现了在无需人工干预条件下丝盘虫个体两个月以上的稳定存活。本研究为丝盘虫这一重要非模式动物的资源保存与长期培养提供了新的实验方案，也为其作为模式体系的进一步应用奠定了技术基础。

Trichoplax adhaerens is one of the earliest-branching extant multicellular animals and represents an important evolutionary model for investigating the origin of animal multicellularity, cell-type evolution, and intercellular coordination. However, under laboratory conditions, Trichoplax cultures are still maintained primarily by manual passaging, and the lack of a reliable medium- to long-term preservation strategy limits both experimental continuity and resource sharing. In this study, we used T. adhaerens Grell strain/H1 to examine temperature-dependent changes in growth, behavior, and gene expression, with the aim of evaluating the feasibility of low-temperature preservation. Compared with conventional culture conditions, cultivation at 16°C markedly reduced locomotor activity and growth rate while preserving basic physiological functions. Behavioral and transcriptomic analyses further indicated that Trichoplax responds to low temperature by downregulating carbohydrate- and lipid-metabolism-related processes, thereby slowing growth and developmental progression. Upon return to standard culture temperature, low-temperature-treated individuals rapidly resumed normal growth activity. Based on these findings, we further optimized a low-temperature preservation strategy that enabled stable survival of Trichoplax for more than two months without manual intervention. This study provides a practical approach for the preservation and long-term maintenance of  Trichoplax, establishing a technical basis for its wider application as an experimental model organism.