刺胞动物作为后生动物早期演化的关键类群，其浮浪幼虫附着变态过程为解析早期多细胞动物的发育遗传规律提供了理想模型。本研究以刺胞动物的代表物种——海月水母（Aurelia coerulea）为研究材料，构建了浮浪幼虫未附着与附着阶段的转录图谱，并结合RT-qPCR和药物干预实验，对关键通路进行验证。结果显示，浮浪幼虫附着前后基因表达模式发生明显变化。功能富集分析表明，附着阶段显著富集发育相关信号通路、细胞表面受体信号通路和转录因子活性等生物学过程，未附着阶段则显著富集钾离子通道活性、钾离子跨膜转运和钠离子通道活性等过程。基因表达分析显示，Wnt信号相关基因在附着阶段显著高表达；药物抑制Wnt信号通路后，变态发育过程受到抑制，幼虫附着率显著降低，表明Wnt信号参与调控海月水母浮浪幼虫的附着变态过程。综上，本研究构建了海月水母浮浪幼虫附着变态关键阶段的转录组图谱，初步揭示了附着变态过程中转录调控特征，为理解海洋无脊椎动物浮浪幼虫附着变态的分子调控基础提供了参考。

As a pivotal group in the early evolution of metazoans, cnidarians serve as an ideal model for exploring the developmental and genetic mechanisms of early multicellular animals through the settlement and metamorphosis of their planula. In this study, the representative cnidarian species, Aurelia coerulea, was used to construct transcriptomic profiles of unattached and attached planula. RT-qPCR and pharmacological inhibition experiments were further performed to validate key regulatory pathways. The results showed substantial changes in gene expression patterns before and after planula attachment. Functional enrichment analysis revealed that genes in the attached stage were significantly enriched in developmental signaling pathways, cell surface receptor signaling pathways, and transcription factor activity, whereas genes in the unattached stage were mainly enriched in potassium ion channel activity, potassium ion transmembrane transport, and sodium ion channel activity. Gene expression analysis demonstrated that genes related to Wnt signaling were significantly upregulated at the attachment stage. Inhibition of the Wnt signaling pathway significantly reduced planula attachment rates and suppressed metamorphosis, indicating that Wnt signaling is involved in regulating the settlement and metamorphosis of A. coerulea planula. Overall, this study provides transcriptomic landscapes of critical stages during the settlement and metamorphosis of A. coerulea planula, preliminarily reveals the transcriptional regulatory characteristics underlying the process, and offers insights into the molecular regulatory mechanisms controlling planula settlement and metamorphosis in marine invertebrates.