遗传 ›› 2007, Vol. 29 ›› Issue (7): 805-812.doi: 10.1360/yc-007-0805

• 研究报告 • 上一篇    下一篇

吉林人群强直性脊柱炎6号染色体短臂上的HLA区域遗传易感基因定位研究

王雅文1, 2; 朱小泉1; 宋玉国2; 孙亮3; 杨泽1   

  1. 1. 北京医院,北京卫生部老年医学研究所, 北京100730;
    2. 北华大学附属医院临床免疫研究室, 吉林 132001;
    3. 中国科学院遗传与发育生物学研究所, 北京 100101

  • 收稿日期:2006-10-30 修回日期:2007-05-21 出版日期:2007-07-10 发布日期:2007-07-10
  • 通讯作者: 王雅文

Mapping study of ankylosing spondylitis on HLA region in Jilin population of China

WANG Ya-Wen1; ZHU Xiao-Quan1; SONG Yu-Guo2; SUN Liang3; YANG Ze1   

  1. 1. Institute of Geriatrics, Ministry of Health, Beijing Hospital, Beijing 100730, China;
    2. Department of Clinic Immune, the Affiliated Hospital of Beihua University, Jilin 132001, China;
    3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101,China
  • Received:2006-10-30 Revised:2007-05-21 Online:2007-07-10 Published:2007-07-10
  • Contact: WANG Ya-Wen

摘要:

为了寻找中国人群中与强直性脊柱炎相关的新的易感基因及其所在位置, 在与强直性脊柱炎强连锁的6 号染色体短臂上的HLA基因区域内选取11个SNPs多态位点, 通过对中国吉林地区79名AS患者和132名正常对照者进行case-control分析, 发现TNF-a -850处TT突变基因型在AS组中的分布高于正常对照组(P=0.027), 突变型T等位基因在AS组和正常对照组中的分布差异更为显著(P=0.002)。通过多位点之间的连锁不平衡分析发现, LTA基因、TNF-a基因、LST1基因和NCR3基因中的 5个SNPs多态位点之间存在连锁不平衡, 范围是15 kb, 在这5个SNPs多态位点组成的单体型中, TCTTC单体型在AS组和正常对照组中的分布有显著差异(c2=7.406, P=0.0065),并且该单体型中含有具有统计学意义的TNF-a –850的突变型等位基因T。提示在LTATNF-aNCR3LST1 这4个基因构成的15 kb范围内可能存在增加AS患病易感性的位点, 可能是TNF-a –850 C→T突变, 也可能是在TNF-a –850附近的其他位点。

关键词: 强直性脊柱炎, 易感基因, 连锁不平衡, 基因型频率

Abstract:

To find the susceptible genes of ankylosing spondylitis in Chinese population, we select 11 SNPs on gene HLA gene family that has strong linkage of ankylosing spondylitis in 6p21.3. By case-control study in 79 AS patients and 132 healthy subjects, the distribution of TNF-a –850 genotype TT is higher in AS group than that in normal control group (P=0.027); Mutational allele T has a significant statistically difference between AS group and normal control group (P=0.002). By linkage disequilibrium study, there are 5 SNPs present the linkage disequilibrium and the region is 15 kb, including gene LTA, TNF-a, LST1 and NCR3; In the haplotypes of the 5 SNPs , the distribution of haplotype TCTTC has statistical difference between AS group and normal control group (c2=7.406, P=0.0065), the haplotype contains mutational allele T of TNF- a -850. The result hints that there may be susceptible sites of AS in this 15 kb region, which may be TNF-a –850 C→T mutation or other sites that around the TNF-a –850.