遗传 ›› 2007, Vol. 29 ›› Issue (9): 1103-1103―1109.doi: 10.1360/yc-007-1103

• 研究报告 • 上一篇    下一篇

8个亚洲水牛群体的遗传结构分析

王冬蕾1, 常洪1, 杨军香2, 张桂香3, 王志刚3, 于波1, 廖信军4, 宋卫涛5, 韩旭3, 宋光明1, 王均辉6, 李荣岭7   

  1. 1. 扬州大学动物科学与技术学院, 扬州 225009;
    2. 全国畜牧总站, 北京 100026;
    3. 全国畜牧兽医总站畜禽牧草种质资源保存利用中心, 北京 100094;
    4. 井冈山学院生命科学学院, 吉安 343000;
    5. 中国农业科学院家禽研究所, 扬州 225003;
    6. 西北农林科技大学, 杨凌 712100;
    7. 山东农业大学动物科学与技术学院, 泰安 271018

  • 收稿日期:2007-03-16 修回日期:2007-05-10 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 王冬蕾

Analysis on the genetic structure of 8 Asia buffalo populations

WANG Dong-Lei1, CHANG Hong1, YANG Jun-Xiang2, ZHANG Gui-Xiang3, WANG Zhi-Gang3, YU Bo1, LIAO Xin-Jun4, SONG Wei-Tao5

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. National Animal Husbandry & Veterinary Service, Beijing 100026, China;
    3. National Center for Preservation and Utilization of Genetic Resources of Domestic Animals and Forages, National Animal Husbandry & Veterinary Service, Beijing 100094, China;
    4. College of Animal Science and Technology, Jinggangshan University, Ji’an 343000, China;
    5. Institute of Poultry, Chinese Academy of Agricultural Sciences, Yangzhou 225003, China;
    6. College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China;
    7. College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271018, China
  • Received:2007-03-16 Revised:2007-05-10 Online:2007-09-10 Published:2007-09-10

PDF (PC)

2320

被引次数

19

摘要：

应用13个微卫星标记结合荧光–多重PCR技术, 对德昌水牛、兴隆水牛、富钟水牛、温州水牛、东流水牛、福安水牛和两个引进品种摩拉水牛、尼里－拉菲水牛进行遗传结构分析。结果表明: 8个水牛群体在13个微卫星座位中共检测到157个等位基因, 其中7个群体具有各自的特有等位基因, 其和为23; 8个群体的有效等位基因数在2.2908～4.2308之间, 杂合度在0.4951～0.7194之间, 多态信息含量在0.4495～0.6776之间; 有11个座位为高度多态座位, 是适合分析水牛遗传多样性的多态标记; 聚类分析表明富钟水牛和东流水牛先聚在一起, 再与兴隆水牛聚在一起, 然后与温州水牛和福安水牛聚在一起, 德昌水牛独自聚为一类; 两个引进品种聚为一类。

关键词: 多重PCR, 遗传多样性, 微卫星, 水牛

Abstract:

One hundred and forty seven alleles were detected when thirteen microsatellite loci were analyzed applying fluorescence-multiplex PCR technology in eight buffalo populations were analyzed, including six indigenous Chinese native breeds (Dechang、Xinglong、Fuzhong、Wenzhou、Dongliu、Fu’an), and two introduced breeds (Murrah、Nili-Ravi). Seven populations have their own unique alleles, total number is twenty-three. As to all the eight populations, effective number of alleles (Ne) was between 2.2908 and 4.2308, heterozygosity (H) between 0.4951 and 0.7194, and polymorphism information content (PIC) between 0.4495 and 0.6776. Eleven of the thirteen microsatellite loci were of high polymorphism and were then the appropriate, polymorphism marker could be used to analyze properly genetic diversity of the involved buffalo populations. Cluster analysis indicated that Fuzhong and Dongliu were clustered together, then with an independent cluster of Xinglong. Wenzhou and Fu’an were clustered together, Dechang was an independent cluster. Murrah and Nili-Ravi were clustered together.

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