饥饿对幼年斑马鱼下丘脑摄食相关性神经肽表达的影响

doi:10.16288/j.yczz.16-087

遗传 ›› 2016, Vol. 38 ›› Issue (9): 821-830.doi: 10.16288/j.yczz.16-087

饥饿对幼年斑马鱼下丘脑摄食相关性神经肽表达的影响

刘姗姗, 张翠珍, 彭刚

复旦大学脑科学研究院,上海 200032

收稿日期:2016-03-14 出版日期:2016-09-20 发布日期:2016-09-20
通讯作者: 彭刚,教授,博士生导师,研究方向：斑马鱼神经系统发育及行为的分子机理。E-mail: gangpeng@fudan.edu.cn
作者简介:刘姗姗,硕士研究生,专业方向：斑马鱼神经系统发育及行为的分子机理。E-mail: 13210700024@fudan.edu.cn
基金资助:
国家自然科学基金项目(编号: 31171074)资助

Effects of starvation on the expression of feeding related neuropeptides in the larval zebrafish hypothalamus

Shanshan Liu, Cuizhen Zhang, Gang Peng

Institute of Brain Science, Fudan University, Shanghai 200032, China

Received:2016-03-14 Online:2016-09-20 Published:2016-09-20
Supported by:
[Supported by the National Nature Science Foundation of China(No; 31171074)]

摘要/Abstract

摘要： 脊椎动物下丘脑中的神经肽Y(Neuropeptide Y, NPY)、GALANIN和GMAP蛋白前体(GALANIN and GMAP prepropeptide, GAL)、Agouti相关蛋白(Agouti related neuropeptide, AGRP)和阿片促黑色素原(Proopiomelanocortin, POMC)与摄食密切相关,但在斑马鱼中对这些神经肽与摄食之间关系的研究较少。本文通过原位杂交技术和实时定量PCR方法,观察饥饿1 d、饥饿2 d和饥饿2 d喂食2 d后斑马鱼下丘脑中npy、galanin、agrp和pomca的表达情况。结果显示,饥饿处理之后,agrp和galanin在斑马鱼下丘脑中的表达量显著上升(P<0.05)。与对照组相比,饥饿2 d后斑马鱼下丘脑中pomca表达量显著下降(P<0.05)。饥饿2 d喂食2 d后斑马鱼下丘脑中pomca、agrp和galanin的表达量与对照组相比没有显著性差异。所有实验中npy在斑马鱼下丘脑中的表达没有显著性差异。这表明饥饿处理促使斑马鱼下丘脑中agrp和galanin表达上调,pomca表达下调;及时摄食可以恢复agrp、galanin和pomca在下丘脑中的表达水平。

关键词: 斑马鱼, npy, galanin, agrp, pomca

Abstract: Vertebrate feeding behavior is regulated by neuropeptide Y (NPY), GALANIN and GMAP prepropeptide (GAL), agouti related neuropeptide (AGRP) and proopiomelanocortin (POMC) in the hypothalamus. However, there are few studies on the relationship between these neuropeptides and feeding in zebrafish larvae. In the present study, real-time quantitative PCR and in situ hybridization were applied to examine the expression levels of npy, galanin, agrp and pomca in the hypothalamus of zebrafish larvae after starvation and re-feeding. The results showed the expression of agrp and galanin increased significantly after starvation compared to the control group, whilst the expression of pomca decreased significantly compared to control. If the animals were re-fed for two days after starvation, the expression of pomca, agrp and galanin showed no significant difference from the control. Expression of npy did not alter in either condition. These results indicate that starvation increases expression levels of agrp and galanin, and reduces the pomca expression. In addition, these starvation-induced changes can be reversed by re-feeding.

Key words: zebrafish, npy, galanin, agrp, pomca

刘姗姗, 张翠珍, 彭刚. 饥饿对幼年斑马鱼下丘脑摄食相关性神经肽表达的影响[J]. 遗传, 2016, 38(9): 821-830.

Shanshan Liu, Cuizhen Zhang, Gang Peng. Effects of starvation on the expression of feeding related neuropeptides in the larval zebrafish hypothalamus[J]. Hereditas(Beijing), 2016, 38(9): 821-830.

参考文献

[1] Bolborea M, Dale N. Hypothalamic tanycytes: potential roles in the control of feeding and energy balance. Trends Neurosci , 2013, 36(2): 91-100.
[2] Larhammar D. Evolution of neuropeptide Y, peptide YY and pancreatic polypeptide. Regul Pept , 1996, 62(1): 1- 11.
[3] Tatemoto K, Carlquist M, Mutt V. Neuropeptide Y-a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature , 1982, 296(5858): 659-660.
[4] Dumont Y, Martel JC, Fournier A, St-Pierre S, Quirion R. Neuropeptide Y and neuropeptide Y receptor subtypes in brain and peripheral tissues. Prog Neurobiol , 1992, 38(2): 125-167.
[5] Williams G, Harrold JA, Cutler DJ. The hypothalamus and the regulation of energy homeostasis: lifting the lid on a black box. Proc Nutr Soc , 2000, 59(3): 385-396.
[6] Yoshitake T, Wang FH, Kuteeva E, Holmberg K, Yamaguchi M, Crawley JN, Steiner R, Bartfai T, Ögren SO, Hökfelt T, Kehr J. Enhanced hippocampal noradrenaline and serotonin release in galanin-overexpressing mice after repeated forced swimming test. Proc Natl Acad Sci USA , 2004, 101(1): 354-359.
[7] Millón C, Flores-Burgess A, Narváez M, Borroto-Escuela DO, Santín L, Parrado C, Narváez JA, Fuxe K, Díaz- Cabiale Z. A role for galanin N-terminal fragment (1-15) in anxiety- and depression-related behaviors in rats. Int J Neuropsychopharmacol , 2015, 18(3), doi:10.1093/ijnp/ pyu064.
[8] Misawa K, Misawa Y, Kanazawa T, Mochizuki D, Imai A, Endo S, Carey TE, Mineta H. Epigenetic inactivation of galanin and GALR1/2 is associated with early recurrence in head and neck cancer. Clin Exp Metastasis , 2016, 33(2): 187-195.
[9] Zafar MI, Hu CN, Liu DF, Shafqat RA, Gao F. Insulin detemir causes lesser weight gain in comparison to insulin glargine: role on hypothalamic NPY and galanin. J Diabetes Res , 2014, 2014: 458104.
[10] Shukla C, Basheer R. Metabolic signals in sleep regulation: recent insights. Nat Sci Sleep , 2016, 8(1): 9-20.
[11] Huang H, Lee SH, Ye CP, Lima IS, Oh BC, Lowell BB, Zabolotny JM, Kim YB. ROCK1 in AgRP neurons regulates energy expenditure and locomotor activity in male mice. Endocrinology , 2013, 154(10): 3660-3670.
[12] Wu YH, Patchev AV, Daniel G, Almeida OFX, Spengler D. Early-life stress reduces DNA methylation of the Pomc gene in male mice. Endocrinology , 2014, 155(5): 1751- 1762.
[13] Anderson EJ, Çakir I, Carrington S, Cone R, Ghamari- Langroudi M, Gillyard T, Gimenez LE, Litt MJ. 60 years of POMC: Regulation of feeding and energy homeostasis by α-MSH. J Mol Endocrinol , 2016, 56(4): T157- T174.
[14] Li L, Luo LF. Zebrafish as the model system to study organogenesis and regeneration. Hereditas ( Beijing ), 2013, 35(4): 421-432. 李礼, 罗凌飞. 以斑马鱼为模式动物研究器官的发育与再生. 遗传, 2013, 35(4): 421-432.
[15] Parker MO, Brock AJ, Walton RT, Brennan CH. The role of zebrafish ( Danio rerio ) in dissecting the genetics and neural circuits of executive function. Front Neural Circuits , 2013, 7: 63.
[16] Morin C, de Souza MA, Müller CP, Hardigan P, Spieler RE. Active avoidance learning in zebrafish ( Danio rerio )- the role of sensory modality and inter-stimulus interval. Behav Brain Res , 2013, 248: 141-143.
[17] Li HH, Huang P, Dong W, Zhu ZY, Liu D. A brief history of zebrafish research-toward biomedicine. Hereditas ( Beijing ), 2013, 35(4): 310-320. 李辉辉, 黄萍, 董巍, 朱作言, 刘东. 斑马鱼研究走向生物医学. 遗传, 2013, 35(4): 310-320.
[18] Kalueff AV, Gebhardt M, Stewart AM, Cachat JM, Brimmer M, Chawla JS, Craddock C, Kyzar EJ, Roth A, Landsman S, Gaikwad S, Robinson K, Baatrup E, Tierney K, Shamchuk A, Norton W, Miller N, Nicolson T, Braubach O, Gilman CP, Pittman J, Rosemberg DB, Gerlai R, Echevarria D, Lamb E, Neuhauss SCF, Weng W, Bally-Cuif L, Schneider H. Towards a comprehensive catalog of zebrafish behavior 1.0 and beyond. Zebra

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

饥饿对幼年斑马鱼下丘脑摄食相关性神经肽表达的影响

Effects of starvation on the expression of feeding related neuropeptides in the larval zebrafish hypothalamus

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	李凯伦, 卢荆奥, 陈小辉, 张文清, 刘伟. 尿囊素促进破骨细胞缺陷斑马鱼骨折修复[J]. 遗传, 2023, 45(4): 341-353.
[2]	卢荆澳, 黄春燕, 林芷茵, 唐政, 马宁, 黄志斌. cd99l2基因调控斑马鱼白细胞组织间的迁移机制[J]. 遗传, 2022, 44(9): 798-809.
[3]	郑鹏飞, 谢海波, 朱盼盼, 赵呈天. 斑马鱼神经底板处神经元的分布及特征[J]. 遗传, 2022, 44(6): 510-520.
[4]	张婷婷, 刘峰. 斑马鱼蛋白酪氨酸硫酸化修饰的检测方法研究[J]. 遗传, 2022, 44(2): 178-186.
[5]	贾婷婷, 雷蕾, 吴歆媛, 蔡顺有, 陈艺璇, 薛钰. 二甲双胍对斑马鱼骨骼发育及损伤修复的机制研究[J]. 遗传, 2022, 44(1): 68-79.
[6]	郭佳妮, 刘帆, 王璐. 斑马鱼血液疾病模型及应用[J]. 遗传, 2020, 42(8): 725-738.
[7]	熊凤,谢训卫,潘鲁媛,李阔宇,柳力月,张昀,李玲璐,孙永华. 国家斑马鱼资源中心的资源、技术和服务建设[J]. 遗传, 2018, 40(8): 683-692.
[8]	许璟瑾, 张文娟, 王静怡, 姚丽云, 潘裕添, 欧一新, 薛钰, . 金线莲抑制斑马鱼黑色素形成的活性组分筛选及机理研究[J]. 遗传, 2017, 39(12): 1178-1187.
[9]	张峰华,王厚鹏,黄思雨,熊凤,朱作言,孙永华. 两种密码子优化的Cas9编码基因在斑马鱼胚胎中基因敲除效率的比较[J]. 遗传, 2016, 38(2): 144-154.
[10]	顾爱华严丽锋. 斑马鱼在再生医学研究中的应用及进展[J]. 遗传, 2013, 35(7): 856-866.
[11]	李礼，罗凌飞. 以斑马鱼为模式动物研究器官的发育与再生[J]. 遗传, 2013, 35(4): 421-432.
[12]	徐冉冉张从伟曹羽王强. 缺失mir122抑制斑马鱼肝脏前体细胞向肝细胞分化[J]. 遗传, 2013, 35(4): 488-494.
[13]	沈延黄鹏张博. TALEN构建与斑马鱼基因组定点突变的实验方法与流程[J]. 遗传, 2013, 35(4): 533-544.
[14]	李辉辉黄萍董巍朱作言刘东. 斑马鱼研究走向生物医学[J]. 遗传, 2013, 35(4): 410-420.
[15]	李小泉，杜久林. 幼年斑马鱼的视觉系统与捕食行为[J]. 遗传, 2013, 35(4): 468-476.