m 6A甲基化与肿瘤研究进展

doi:10.16288/j.yczz.18-098

遗传 ›› 2018, Vol. 40 ›› Issue (12): 1055-1065.doi: 10.16288/j.yczz.18-098

• 综述 • 下一篇

m ⁶A甲基化与肿瘤研究进展

王天工¹,叶孟²()

^1. 宁波大学医学院,宁波 315211
^2. 宁波大学医学院附属医院,宁波 315211

收稿日期:2018-05-18 修回日期:2018-07-31 出版日期:2018-12-20 发布日期:2018-09-25
作者简介:王天工,硕士研究生,专业方向：肿瘤学。E-mail: tonywoolf21@gmail.com
基金资助:
浙江省自然科学基金项目(编号：Y16H160019)资助(Y16H160019)

Advances on the roles of m ⁶A in tumorigenesis

Tiangong Wang¹,Meng Ye²()

^1. Medical School of Ningbo University, Ningbo 315211, China
^2. The Affiliated Hospital of Medical School of Ningbo University, Ningbo 315211, China

Received:2018-05-18 Revised:2018-07-31 Published:2018-12-20 Online:2018-09-25
Supported by:
Supported by Zhejiang Provincial Natural Science Foundation of China(Y16H160019)

摘要/Abstract

摘要：

m ⁶A甲基化是20世纪70年代被发现的一种RNA分子上的甲基化修饰,存在于各种RNA中,但主要在mRNA中出现。与DNA甲基化相似,m ⁶A甲基化也可以在不改变碱基序列的情况下对基因的转录后表达水平具有调控作用。它主要是通过影响mRNA与读取蛋白的结合,从而调控mRNA的可变剪接、翻译效率和稳定性等,进而改变基因表达。研究早期,受限于m ⁶A甲基化位点的检测技术不够灵敏,转录组中的m ⁶A位点无法被完整而有效地鉴别。随着二代测序与生物信息学的发展,已有多种方法可以对m ⁶A甲基化位点进行检测和预测。目前的研究表明,m ⁶A甲基化与肿瘤的发生发展也密切相关。本文结合近期的研究进展,对m ⁶A甲基化的概念、检测和预测手段,特别是m6A与肿瘤发生之间的关系进行了综述,旨在为肿瘤的分子病理诊断和分子靶向治疗寻找新方向。

关键词: m ⁶A甲基化, RNA甲基化, 表观遗传, 肿瘤

Abstract:

Predominantly found in mRNA, m ⁶A methylation of RNA molecules regulates post-transcriptional gene expression without changing RNA sequence. m ⁶A methylation alters gene expression by modulating mRNA processing and metabolism, including alternative splicing, translation efficiency, and stability. Current research shows that m ⁶A methylation is involved in tumorigenesis, highlighting the importance of further study. However, traditional methods are not sensitive enough to detect the full patterns of m ⁶A methylation in the transcriptome. The development of new technologies, such as single-nucleotide detection combined with next generation sequencing, allows for the quick and accurate prediction and identification of m ⁶A methylation sites. In this review, we summarize the recent progress of m ⁶A in tumorigenesis and outline a new direction for both molecular pathology diagnosis and targeted therapy of tumor.

Key words: m ⁶A, RNA methylation, epigenetics, tumor

王天工, 叶孟. m ⁶A甲基化与肿瘤研究进展[J]. 遗传, 2018, 40(12): 1055-1065.

Tiangong Wang, Meng Ye. Advances on the roles of m ⁶A in tumorigenesis[J]. Hereditas(Beijing), 2018, 40(12): 1055-1065.

参考文献

[1]	Dubin DT, Taylor RH . The methylation state of poly A-containing messenger RNA from cultured hamster cells . Nucleic Acids Res, 1975,2(10):1653-1668.
[2]	Lee M, Kim B, Kim VN . Emerging roles of RNA modification: m 6A and U-tail . Cell, 2014,158(5):980-987.
[3]	Wei W, Ji X, Guo X, Ji S . Regulatory Role of N 6- methyladenosine (m 6 A) Methylation in RNA processing and human diseases . J Cell Biochem, 2017,118(9):2534-2543.
[4]	Zhang X, Jia GF . RNA epigenetic modification: N 6- methyladenosine. Hereditas(Beijing) , 2016,38(4):275-288.
[4]	张笑, 贾桂芳 . RNA表观遗传修饰:N 6-甲基腺嘌呤 . 遗传, 2016,38(4):275-288.
[5]	Li YL, Yu J, Song SH . Recent progresses in RNA N6-methyladenosine research. Hereditas(Beijing), 2013,35(12):1340-1351.
[5]	李语丽, 于军, 宋述慧 . RNA中6-甲基腺嘌呤的研究进展. 遗传, 2013,35(12):1340-1351.
[6]	Visvanathan A , Somasundaram K.mRNA traffic gontrol reviewed: N6-Methyladenosine (m 6 A) takes the driver's seat . Bioessays, 2018,40(1). doi: 10.1002/bies.201700093.
[7]	Jacob R, Zander S , Gutschner T. The Dark side of the epitranscriptome: chemical modifications in long non- coding RNAs . Int J Mol Sci, 2017, 18(11): pii: E2387.
[8]	Alarcón CR, Lee H, Goodarzi H, Halberg N, Tavazoie SF . N6-methyladenosine marks primary microRNAs for processing . Nature, 2015,519(7544):482-485.
[9]	Berulava T, Rahmann S, Rademacher K, Klein-Hitpass L, Horsthemke B , N6-adenosine methylation in MiRNAs . PLoS One, 2015,10(2):e0118438.
[10]	Bokar JA, Shambaugh ME, Polayes D, Matera AG, Rottman FM . Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase . RNA, 1997,3(11):1233-1247.
[11]	Wang Y, Li Y, Toth JI, Petroski MD, Zhang Z, Zhao JC . N6-methyladenosine modification destabilizes developmental regulators in embryonic stem cells . Nat Cell Biol, 2014,16(2):191-198.
[12]	Ping XL, Sun BF, Wang L, Xiao W, Yang X, Wang WJ , S Adhikari,Shi Y, Lv Y, Chen YS, Zhao X, Li A, Yang Y, U Dahal, Lou XM, Liu X, Huang J, Yuan WP, Zhu XF, Cheng T, Zhao YL, Wang X, Rendtlew Danielsen JM, Liu F, Yang YG. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase . Cell Res, 2014,24(2):177-189.
[13]	Horiuchi K, Kawamura T, Iwanari H, Ohashi R, Naito M, Kodama T, Hamakubo T . Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle . J Biol Chem, 2013,288(46):292-302.
[14]

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

m ⁶A甲基化与肿瘤研究进展

Advances on the roles of m ⁶A in tumorigenesis

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics