遗传 ›› 2019, Vol. 41 ›› Issue (12): 1148-1155.doi: 10.16288/j.yczz.19-149

• 遗传学教学 • 上一篇    

Ds插入突变体的遗传学综合性实验设计与探讨

李楠1, 李亚娟1, 郭海滨1, 张向前2()   

  1. 1. 华南农业大学公共基础课实验教学中心,广州 510642
    2. 华南农业大学林学与风景园林学院,广州 510642
  • 收稿日期:2019-09-26 修回日期:2019-11-01 出版日期:2019-12-20 发布日期:2019-11-06
  • 通讯作者: 张向前 E-mail:aacrav@163.com
  • 作者简介:李楠,硕士,实验师,研究方向:遗传学实验教学与管理。E-mail:523155900@qq.com
  • 基金资助:
    国家自然科学基金面上项目编号(30900884);国家自然科学基金面上项目编号(31671594);华南农业大学2017年教学改革与研究项目资助编号(JG17093)

Design and exploration of genetic comprehensive experiments based on Ds insertion mutants

Li Nan1, Li Yajuan1, Guo Haibin1, Zhang Xiangqian2()   

  1. 1. Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, China
    2. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
  • Received:2019-09-26 Revised:2019-11-01 Online:2019-12-20 Published:2019-11-06
  • Contact: Zhang Xiangqian E-mail:aacrav@163.com
  • Supported by:
    Supported by the National Natural Science Foundation of China Nos(30900884);Supported by the National Natural Science Foundation of China Nos(31671594);Teaching Reform and Research Projects of SCAU in 2017 No(JG17093)

摘要:

学生创新能力的培养已成为中国高等教育的重要导向,加强综合设计性实验的应用是提高大学生创新能力的有效途径。文章以水稻Ds插入突变体为实验材料,以突变体表型观测作为实验切入点,以遗传分析和Ds转座检测为实验学习重点,重新设计了一个分子遗传学综合性实验项目。在此基础上,通过知识拓展环节引导学生利用开放实验平台学习TAIL-PCR技术,进一步进入研究性实验。通过综合性实验到研究性实验的递进式教学体系,能够使学生深入理解表型与基因的内在联系、跳跃基因的概念以及转座子在基因功能研究中的重要作用,加深学生对基因概念及遗传规律的认识和理解,提升学生理论知识与实验技能的整合运用能力。

关键词: 综合性实验, 实验教学, 基因突变

Abstract:

The cultivation of innovative abilities has become an important guide for higher education in China. Strengthening the integrated knowledge to design experiments is an effective way to improve undergraduate students’ innovative abilities. Herein we designed a comprehensive experiment for molecular genetics by utilizing a rice Ds insertion mutant identified previously in our research project. In the comprehensive experiment, we adopt the method of scientific research as the main line of teaching and take the interesting phenotype of the rice mutant as the breakthrough point to reform and innovate genetics laboratory teaching. On the basis of this, we combined the progressive teaching method and guided the students to learn the TAIL-PCR skill and conduct an innovative experiment through expanding their knowledge. The comprehensive experiment will deepen students’ understandings of the relationship between genotypes and phenotypes, help them master the effective way of thinking and technologies for scientific research to further improve their ability of the integrated application capability of theory and practices.

Key words: comprehensive experiment, experimental teaching, gene mutation