Ds插入突变体的遗传学综合性实验设计与探讨

doi:10.16288/j.yczz.19-149

遗传 ›› 2019, Vol. 41 ›› Issue (12): 1148-1155.doi: 10.16288/j.yczz.19-149

• 遗传学教学 • 上一篇

Ds插入突变体的遗传学综合性实验设计与探讨

李楠¹, 李亚娟¹, 郭海滨¹, 张向前²()

^1. 华南农业大学公共基础课实验教学中心,广州 510642
^2. 华南农业大学林学与风景园林学院,广州 510642

收稿日期:2019-09-26 修回日期:2019-11-01 出版日期:2019-12-20 发布日期:2019-11-06
通讯作者: 张向前 E-mail:aacrav@163.com
作者简介:李楠,硕士,实验师,研究方向：遗传学实验教学与管理。E-mail:523155900@qq.com
基金资助:
国家自然科学基金面上项目编号(30900884);国家自然科学基金面上项目编号(31671594);华南农业大学2017年教学改革与研究项目资助编号(JG17093)

Design and exploration of genetic comprehensive experiments based on Ds insertion mutants

Nan Li¹, Yajuan Li¹, Haibin Guo¹, Xiangqian Zhang²()

^1. Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, China
^2. College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China

Received:2019-09-26 Revised:2019-11-01 Online:2019-12-20 Published:2019-11-06
Contact: Zhang Xiangqian E-mail:aacrav@163.com
Supported by:
Supported by the National Natural Science Foundation of China Nos(30900884);Supported by the National Natural Science Foundation of China Nos(31671594);Teaching Reform and Research Projects of SCAU in 2017 No(JG17093)

摘要/Abstract

摘要：

学生创新能力的培养已成为中国高等教育的重要导向,加强综合设计性实验的应用是提高大学生创新能力的有效途径。文章以水稻Ds插入突变体为实验材料,以突变体表型观测作为实验切入点,以遗传分析和Ds转座检测为实验学习重点,重新设计了一个分子遗传学综合性实验项目。在此基础上,通过知识拓展环节引导学生利用开放实验平台学习TAIL-PCR技术,进一步进入研究性实验。通过综合性实验到研究性实验的递进式教学体系,能够使学生深入理解表型与基因的内在联系、跳跃基因的概念以及转座子在基因功能研究中的重要作用,加深学生对基因概念及遗传规律的认识和理解,提升学生理论知识与实验技能的整合运用能力。

关键词: 综合性实验, 实验教学, 基因突变

Abstract:

The cultivation of innovative abilities has become an important guide for higher education in China. Strengthening the integrated knowledge to design experiments is an effective way to improve undergraduate students’ innovative abilities. Herein we designed a comprehensive experiment for molecular genetics by utilizing a rice Ds insertion mutant identified previously in our research project. In the comprehensive experiment, we adopt the method of scientific research as the main line of teaching and take the interesting phenotype of the rice mutant as the breakthrough point to reform and innovate genetics laboratory teaching. On the basis of this, we combined the progressive teaching method and guided the students to learn the TAIL-PCR skill and conduct an innovative experiment through expanding their knowledge. The comprehensive experiment will deepen students’ understandings of the relationship between genotypes and phenotypes, help them master the effective way of thinking and technologies for scientific research to further improve their ability of the integrated application capability of theory and practices.

Key words: comprehensive experiment, experimental teaching, gene mutation

李楠, 李亚娟, 郭海滨, 张向前. Ds插入突变体的遗传学综合性实验设计与探讨[J]. 遗传, 2019, 41(12): 1148-1155.

Nan Li, Yajuan Li, Haibin Guo, Xiangqian Zhang. Design and exploration of genetic comprehensive experiments based on Ds insertion mutants[J]. Hereditas(Beijing), 2019, 41(12): 1148-1155.

图/表 3

图1

表1

图2

参考文献 15

[1]	Chen DF, Lu DR, Zhang FX, Zhang GF . The development of genetics teaching in China in the last four decades and its future prospect. Hereditas(Beijing), 2018,40(10):916-932. doi: 10.16288/j.yczz.18-171 pmid: 30369473
	陈德富, 卢大儒, 张飞雄, 张根发 . 中国遗传学教学40年发展及展望. 遗传, 2018,40(10):916-932. doi: 10.16288/j.yczz.18-171 pmid: 30369473
[2]	Li N, Guo HB, Li YJ, Lao MM . Design and practice of comprehensive experiment for cytogenetics. Res Explor Lab, 2019,38(9): 200-203,227.
	李楠, 郭海滨, 李亚娟, 劳媚媚 . 细胞遗传学综合性实验的设计与实践. 实验室研究与探索, 2019,38(09):200-203, 227.
[3]	Liu ZQ, Zhao YX, Fu XL, Li N . Design and exploration of genetic experiments for non-mendelian segregation. Hereditas(Beijing), 2019,41(3):262-270.
	刘自强, 赵苑秀, 傅雪琳, 李楠 . 偏孟德尔分离的遗传学实验设计与探讨. 遗传, 2019,41(3):262-270.
[4]	Qi P, Lin YS, Song XJ, Shen JB, Huang W, Shan JX, Zhu MZ, Jiang LW, Gao JP, Lin HX . The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3. Cell Res, 2012,22(12):1666-1680. doi: 10.1038/cr.2012.151
[5]	Segami S, Kono I, Ando T, Yano M, Kitano H, Miura K, Iwasaki Y . Small and round seed 5 gene encodes alpha-tubulin regulating seed cell elongation in rice. Rice, 2012,5(1):4. doi: 10.1186/1939-8433-5-4 pmid: 24764504
[6]	Cho SH, Yoo SC, Zhang H, Pandeya D, Koh HJ, Hwang JY, Kim GT, Paek NC . The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development. New Phytol, 2013,198(4):1071-1084. doi: 10.1111/nph.12231 pmid: 23551229
[7]	Jin J, Shi JL, Liu B, Liu YC, Huang Y, Yu Y, Dong AW . MORF-RELATED GENE702, a reader protein of trimethylated histone H3 lysine 4 and histone H3 lysine 36, Is involved in brassinosteroid-regulated growth and flowering time control in rice. Plant Physiol, 2015,168(4):1275-1285. doi: 10.1104/pp.114.255737 pmid: 25855537
[8]	Liu Y, Whittier RF . Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics, 1995,25(3):674-681. doi: 10.1016/0888-7543(95)80010-j pmid: 7759102
[9]	Liu F, Zhang XQ, Zhang ZM, Chen ZG, Zhu HT, Wang J, Zhang JL, Zhang GQ . Transpositional behaviour of the Ds element in the Ac/Ds system in rice. Chin Sci Bull, 2007,52(20):2789-2796. doi: 10.1007/s00122-003-1416-8 pmid: 14513217
[10]	Liu XD, Li YJ. General Genetics Comprehensive Experiment. Beijing: China Agriculture Press, 2011.
	刘向东, 李亚娟 . 普通遗传学综合性实验. 北京: 中国农业出版社, 2011.
[11]	Jeon JS, An G . Gene tagging in rice: a high throughput system for functional genomics. Plant Sci, 2001,161(2):211-219. doi: 10.1016/s0168-9452(01)00414-9 pmid: 11448751
[12]	Park SH, Kim CM, Je BI, Park SH, Park SJ, Piao HL, Xuan YH, Choe MS, Satoh K, Kikuchi S, Lee KH, Cha YS, Ahn BO, Ji HS, Yun DW, Lee MC, Suh SC, Eun MY, Han CD . A Ds-insertion mutant of OSH6(Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice. Planta, 2007,227(1):1-12. doi: 10.1007/s00425-007-0576-1
[13]	Zhu QH, Dennis ES, Upadhyaya NM . Compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice ( Oryza sativa L.). Plant Cell Rep, 2007,26(4):421-427. doi: 10.1007/s00299-006-0259-6
[14]	Qiang GR, Wang H, Yang ZP, Wang HB, Fang WJ . Research and practice on teaching methods of college organic chemistry experiment. Res Explor Lab, 2017,36(03):200-202, 211.
	强根荣, 王红, 杨振平, 王海滨, 方文军 . 大学有机化学实验教学方法研究与实践. 实验室研究与探索, 2017,36(03):200-202, 211.
[15]	Zu Q, Liu JB, Sun YM, Hu K . Reform of experimental teaching method in colleges and universities. Exper Sci Technol, 2015,13(03):88-90.
	祖强, 刘加彬, 孙岳明, 胡凯 . 大学本科实验教学方法改革初析. 实验科学与技术, 2015,13(03):88-90.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

引物	引物序列(5′→3′)	实验目的	备注
P10	TCCCGTCCGATTTCGACTTTA	Ds转座检测
Tra4	TAGCTCACTCATTAGGCACCC	Ds转座检测
P5	AAGCTCAAGCTGCTCTAGCATTCG	Ds转座检测
Ds3′-1a	GGTTCCCGTCCGATTTCGACT	TAIL-PCR	DsR-primer
Ds3′-2a	CGATTACCGTATTTATCCCGTTC	TAIL-PCR	DsR-primer
Ds3′-3a	TCGTTTCCGTCCCGCAAGT	TAIL-PCR	DsR-primer
AD3	WGTGNAGWANCANAGA	TAIL-PCR	AD primer
AD13	NTSGASNTCNGAATCA	TAIL-PCR	AD primer

Ds插入突变体的遗传学综合性实验设计与探讨

Design and exploration of genetic comprehensive experiments based on Ds insertion mutants

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 3

参考文献 15

相关文章 15

编辑推荐

Metrics

[1]	安钧浩, 赵雪莹, 乔守怡, 卢大儒, 皮妍. 现代计算机技术在遗传学实验教学中的应用——移动端轻量级高精度果蝇遗传性状批量识别系统的开发[J]. 遗传, 2023, 45(4): 354-363.
[2]	宋绍征, 何正义, 成勇, 于宝利, 张婷, 李丹. TALENs介导MSTN基因突变山羊的制备及性能分析[J]. 遗传, 2022, 44(6): 531-542.
[3]	贾觉睿智, 肖诚, 刘艺文, 李冉, 张化冰, 于淼. 二例GCK基因突变致先天性高胰岛素性低血糖症的诊疗和基因检测分析[J]. 遗传, 2022, 44(11): 1056-1062.
[4]	肖诚, 刘洁颖, 杨春如, 于淼. LMNA基因突变相关脂肪萎缩综合征的研究进展[J]. 遗传, 2022, 44(10): 913-925.
[5]	张向前, 李楠, 解新明. 表观遗传学综合性实验设计与探讨[J]. 遗传, 2021, 43(12): 1179-1187.
[6]	蒋琬姿, 张丽雯, 贺彩红, 阮梅花, 季勇, 于建荣, 周红文. 家族性高胆固醇血症研究进展[J]. 遗传, 2021, 43(11): 1011-1022.
[7]	朱海美, 黄鹏程, 赵田田, 周长慧, 李若婉, 于春荣, 陈志勇, 顾林峰, 常艳. 纳米银颗粒及纳米二氧化钛颗粒的体外遗传毒性研究[J]. 遗传, 2020, 42(12): 1192-1200.
[8]	孙兆庆, 闫波. 转录因子GATA6在心血管疾病中的作用及其调控机制[J]. 遗传, 2019, 41(5): 375-383.
[9]	刘自强,赵苑秀,傅雪琳,李楠. 偏孟德尔分离的遗传学实验设计与探讨[J]. 遗传, 2019, 41(3): 262-270.
[10]	徐福如, 蒋文君, 张涛, 姜倩, 张瑞雪, 毕宏生. FBN2基因突变与遗传性结缔组织病的发生[J]. 遗传, 2019, 41(10): 919-927.
[11]	严婷婷, 张蕾, 李余动, 梁新乐. 基于微信的“微生物遗传育种实验”混合式教学模式探究[J]. 遗传, 2018, 40(7): 601-606.
[12]	马小英,赵颖岚,贾方兴,宋亚坤,谢宇聪. 秀丽隐杆线虫在高校遗传学实验中的应用[J]. 遗传, 2017, 39(8): 763-768.
[13]	刘玉庆, 朱雄, 李姝锦, 杨业明, 杨牧, 赵培泉, 朱献军. 家族性渗出性玻璃体视网膜病变患者LRP5基因突变研究[J]. 遗传, 2017, 39(3): 241-249.
[14]	邹永新,龚瑶琴. 影响RNA剪接的基因变异[J]. 遗传, 2017, 39(3): 200-207.
[15]	赵健, 胡冬梅, 于大德, 董明亮, 李云, 范英明, 王延伟, 张金凤. 人类血型性状综合遗传大实验的设计与教学实践[J]. 遗传, 2016, 38(5): 461-466.