遗传 ›› 2020, Vol. 42 ›› Issue (4): 347-353.doi: 10.16288/j.yczz.19-282

• 综述 • 上一篇    下一篇

ATAC-seq在复杂疾病研究中的应用进展

陈敏1, 张峥2, 孟紫媛1, 张学军1()   

  1. 1. 安徽医科大学第一附属医院皮肤性病科,合肥 230022
    2. 复旦大学附属华山医院皮肤性病科,上海 201100
  • 收稿日期:2019-12-10 修回日期:2020-02-16 出版日期:2020-04-20 发布日期:2020-03-06
  • 通讯作者: 张学军 E-mail:ayzxj@vip.sina.com
  • 作者简介:陈敏,硕士研究生,专业方向:皮肤遗传。E-mail: 626074054@qq.com
  • 基金资助:
    国际(地区)合作与交流项目资助编号(81320108016)

ATAC-seq and its applications in complex disease

Min Chen1, Zheng Zhang2, Ziyuan Meng1, Xuejun Zhang1()   

  1. 1. Department of Dermatology and Venerology, the First Affiliated Hospital of Anhui Medical University, Heifei 230022, China
    2. Department of Dermatology and Venerology, Huashan Hospital Affiliated to Fudan University, Shanghai 201100, China
  • Received:2019-12-10 Revised:2020-02-16 Online:2020-04-20 Published:2020-03-06
  • Contact: Zhang Xuejun E-mail:ayzxj@vip.sina.com
  • Supported by:
    Supported by the International Regional Cooperation and Exchange Programs No(81320108016)

摘要:

染色质转座酶可及性测序(assay for transposase-accessible chromatin with high-throughput sequencing,ATAC-seq)是利用Tn5转座酶研究染色质可及性的高通量测序技术。ATAC-seq可以在全基因组范围内绘制染色质可及性图谱,揭示转录因子结合位点以及核小体的位置。在医学领域,ATAC-seq技术是研究重大疾病发病机制、药物作用机制、新药研发和生物标志物功能等的新一代有力工具。本文对ATAC-seq技术的优势及其在复杂疾病研究中的应用和前景进行了综述,以期为人类复杂疾病基因表达调控机制等相关研究的开展提供借鉴与参考。

关键词: ATAC-seq, 染色质可及性, Tn5转座酶, 转录调控水平

Abstract:

ATAC-seq is a high-throughput technology that defines and quantifies chromatin accessibility by analyzing Tn5 transposase enzymes. ATAC-seq is used to map chromatin accessibility genome-wide and to identify regions of transcription-factor binding and nucleosome position. As such, ATAC-seq is a new generation tool used in biomedical research to measure and articulate the pathogenesis of major diseases, to demonstrate the pharmacology of current drugs, and to guide the development of new drugs and the function of biomarkers. In this review, we summarize the current applications and advantages of ATAC-seq, and define its prospective contributions related to the regulatory mechanism of gene expression to identify and manage complex disease while elucidating and guiding future research references and strategies.

Key words: ATAC-seq, chromatin accessibility, Tn5 transposase, transcriptional level