外显子拼接复合体塑造m6A表观转录组的形成

doi:10.16288/j.yczz.23-051

遗传 ›› 2023, Vol. 45 ›› Issue (6): 464-471.doi: 10.16288/j.yczz.23-051

外显子拼接复合体塑造m⁶A表观转录组的形成

宋鹏辉¹(), 马丽娟²(), 严冬¹()

1.复旦大学生命科学学院，遗传工程国家重点实验室，上海 200438
2.中国科学院分子植物科学卓越创新中心，中国科学院大学，上海 200032

收稿日期:2023-03-09 修回日期:2023-05-17 出版日期:2023-06-20 发布日期:2023-05-22
通讯作者: 严冬 E-mail:21110700039@m.fudan.edu.cn;malijuan@cemps.ac.cn;yandong@fudan.edu.cn
作者简介:宋鹏辉，在读博士研究生，专业方向：遗传学。E-mail: 21110700039@m.fudan.edu.cn|马丽娟，在读博士研究生，专业方向：生物化学和分子生物学。E-mail: malijuan@cemps.ac.cn;
宋鹏辉和马丽娟并列第一作者。
基金资助:
国家自然科学基金项目(31970786);国家自然科学基金项目(32270868)

Exon junction complex modulates the formation of the m⁶A epitranscriptome

Penghui Song¹(), Lijuan Ma²(), Dong Yan¹()

1. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China
2. CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200032, China

Received:2023-03-09 Revised:2023-05-17 Online:2023-06-20 Published:2023-05-22
Contact: Yan Dong E-mail:21110700039@m.fudan.edu.cn;malijuan@cemps.ac.cn;yandong@fudan.edu.cn
Supported by:
National Natural Science Foundation of China(31970786);National Natural Science Foundation of China(32270868)

摘要/Abstract

摘要：

N⁶-甲基腺嘌呤(N⁶-methyladenosine，m⁶A)是mRNA中含量最丰富的化学修饰之一，在各种生理病理过程中发挥关键性的作用。m⁶A修饰主要位于mRNA终止密码子附近和长的内部外显子上，然而导致这一特异性分布的机制却一直不清楚。近期发表的3篇论文揭示了外显子拼接复合体(exon junction complexes，EJCs)作为m⁶A修饰的抑制蛋白suppressors，塑造了m⁶A表观转录组的形成，解决了这一重大问题。由此，本文简要介绍了m⁶A修饰通路，并结合这些研究成果阐述了EJC对m⁶A修饰形成的作用和机理，进而说明外显子-内含子结构通过m⁶A修饰影响mRNA的稳定性，以期理解m⁶A这一RNA表观修饰领域的最新进展。

关键词: RNA修饰, m⁶A, 外显子拼接复合体, 剪接, RNA稳定性

Abstract:

N⁶-methyladenosine (m⁶A) is one of the most abundant chemical modifications in mRNA and plays essential roles in diverse physiological and pathological processes. m⁶A is highly enriched near stop codons and in long internal exons of mRNA, but the mechanism leading to this specific distribution has been unclear. Recently, three papers have solved this major problem by revealing that exon junction complexes (EJCs) act as m⁶A suppressors and shape the formation of the m⁶A epitranscriptome. Here, we briefly introduce the m⁶A pathway, elaborate the roles of EJC on the formation of m⁶A modification based on these results, and describe the effect of exon-intron structure on mRNA stability via m⁶A, which will help us better understand the latest progress in the m⁶A RNA modification field.

Key words: RNA modification, m⁶A, exon junction complexes, splicing, RNA stability

宋鹏辉, 马丽娟, 严冬. 外显子拼接复合体塑造m⁶A表观转录组的形成[J]. 遗传, 2023, 45(6): 464-471.

Penghui Song, Lijuan Ma, Dong Yan. Exon junction complex modulates the formation of the m⁶A epitranscriptome[J]. Hereditas(Beijing), 2023, 45(6): 464-471.

图/表 1

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