遗传 ›› 2009, Vol. 31 ›› Issue (3): 297-304.doi: 10.3724/SP.J.1005.2009.00297

• 研究报告 • 上一篇    下一篇

叶锈菌胁迫下的小麦基因组MSAP分析

付胜杰1, 3;王晖2;冯丽娜1;孙一1;杨文香1;刘大群1   

  1. 1. 河北农业大学植物保护学院植物病理系, 河北省农作物病虫害生物防治工程技术研究中心, 保定 071001;
    2. 济宁医学院, 济宁 272013;
    3. 山东省济宁市梁山县农业办公室,梁山272600
  • 收稿日期:2008-05-30 修回日期:2008-07-29 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 杨文香;刘大群

Analysis of methylation-sensitive amplified polymorphism in wheat genome under the wheat leaf rust stress

FU Sheng-Jie1, 3;WANG Hui2;FENG Li-Na1;SUN Yi;YANG Wen-Xiang1;LIU Da-Qun1   

  1. 1. Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Biological Control Center for Plant Dis-eases and Plant Pests of Hebei Provinec, Baoding 071001, China;
    2. Jining Medical College, Jining 272013, China;
    3. Liangshan Agricultural Office of Shandong Province, Liangshan 272600, China
  • Received:2008-05-30 Revised:2008-07-29 Online:2009-03-10 Published:2009-03-10
  • Contact: YANG Wen-Xiang

摘要: 内源DNA甲基化是真核生物表观遗传调控的重要组成部分, 在真核生物的基因表达调控中具有重要的作用。生物胁迫为植物提供一种内在的表观遗传进化动力。研究生物胁迫下DNA甲基化的变异模式, 有助于全面理解DNA甲基化的表观调控生物学功能。小麦近等基因系TcLr19、TcLr41及其感病亲本Thatcher在苗期对叶锈菌生理小种THTT、TKTJ分别表现为小种特异性抗病反应和感病反应。文章利用甲基化敏感扩增多态性(Methylation-sensitive amplified polymorphism, MSAP)技术分析了小麦的甲基化水平, 同时比较了苗期在生物胁迫前后基因组DNA胞嘧啶甲基化模式。用60对MSAP引物对接种前后的小麦DNA进行全基因组筛选, 没有直接分离得到接菌前后的甲基化模式的差异, 结果初步表明, 叶锈菌并没有诱导稳定且特异的植物基因组DNA胞嘧啶位点的甲基化模式变化, 但发现TcLr41及其感病亲本Thatcher之间存在表观遗传学差异。

关键词: 甲基化敏感扩增多态性(MSAP), 抗病性, 小麦叶锈菌, DNA甲基化

Abstract: Intrinsic DNA methylation pattern is an integral component of the epigenetic network in many eukaryotes. DNA methylation plays an important role in regulating gene expression in eukaryotes. Biological stress in plant pro-vides an inherent epigenetic driving force of evolution. Study of DNA methylation patterns arising from biological stress will help us fully understand the epigenetic regulation of gene expression and DNA methylation of biological functions. The wheat near-isogenic lines TcLr19 and TcLr41 were resistant to races THTT and TKTJ, respectively, and Thatcher is compatible in the interaction with Puccinia triticina THTT and TKTJ, respectively. By means of me-thylation-sensitive amplified polymorphism (MSAP) analysis, the patterns of cytosine methylation in TcLr19, TcLr41, and Thatcher inoculated with P. triticina THTT and TKTJ were compared with those of the untreated samples. All the DNA fragments, each representing a recognition site cleaved by each or both of isoschizomers, were amplified using 60 pairs of selective primers. The results indicated that there was no significant difference between the challenged and unchallenged plants at DNA methylation level. However, epigenetic difference between the near-isogenic line for wheat leaf rust resistance gene Lr41 and Thatcher was present.