长链非编码RNA的作用机制及其研究方法

doi:10.3724/SP.J.1005.2013.00269

遗传 ›› 2013, Vol. 35 ›› Issue (3): 269-280.doi: 10.3724/SP.J.1005.2013.00269

长链非编码RNA的作用机制及其研究方法

夏天, 肖丙秀, 郭俊明

宁波大学医学院生物化学与分子生物学研究所, 浙江省病理生理学技术研究重点实验室, 宁波 315211

收稿日期:2012-08-08 修回日期:2012-09-20 出版日期:2013-03-20 发布日期:2013-03-25
通讯作者: 郭俊明 E-mail:junmingguo@yahoo.com
基金资助:
国家自然科学基金项目(编号：81171660), 宁波市自然科学基金项目(编号：2012A610207), 宁波市科技创新团队项目(编号：2011B82014), 宁波市重点学科项目(编号：XKL11D2127)和宁波大学优秀学位论文培育基金项目(编号：PY2012004)资助

Acting mechanisms and research methods of long noncoding RNAs

XIA Tian, XIAO Bing-Xiu, GUO Jun-Ming

Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo 315211, China

Received:2012-08-08 Revised:2012-09-20 Online:2013-03-20 Published:2013-03-25

摘要/Abstract

摘要： 长链非编码RNA(Long non-coding RNA, lncRNA)通过多种机制发挥其生物学功能, 这些机制包括基因印记、染色质重塑、细胞周期调控、剪接调控、mRNA降解和翻译调控等。lncRNA通过这些作用机制在不同水平进行基因表达调控。在研究lncRNA功能的过程中, 研究方法的建立和应用起着非常重要的作用。目前用于lncRNA研究的主要方法有：微阵列、转录组测序、Northern印迹、实时荧光定量逆转录-聚合酶链反应、荧光原位杂交、RNA干扰和RNA结合蛋白免疫沉淀等。文章着重介绍了3种前沿方法, 即：在线快速预测RNA与蛋白质相互作用的catRAPID、RNA纯化的染色质分离(Chromatin isolation by RNA purification, ChIRP)以及非编码RNA沉默与定位分析技术(Combined knockdown and localization analysis of non-coding RNAs, c-KLAN)。

关键词: 基因表达调控, 研究方法, 长链非编码RNA, 作用机制

Abstract: Long non-coding RNAs (lncRNAs) play biological roles through a variety of mechanisms, including genetic imprinting, chromatin remodeling, cell cycle control, splicing regulation, mRNA decay, and translational regulation. LncRNAs are involved in the regulation of gene expression through the above mechanisms in different levels. Establishment and application of research technologies are important in understanding of lncRNAs functions. Microarray, RNA sequencing, Northern blot, real time quantitative reverse transcription-polymerase chain reaction, fluorescence in situ hybridization, RNA interference, and RNA-binding protein immunoprecipitation are major tools of exploring biological functions of lncRNAs. Here, we highlighted three advanced methods, i.e., fast predictions of RNA and protein interactions and domains (catRAPID), chromatin isolation by RNA purification (ChIRP), and combined knockdown and localization analysis of non-coding RNAs (c-KLAN).

Key words: gene expression regulation, research methods, long non-coding RNAs (lncRNAs), acting mechanisms

中图分类号:

夏天，肖丙秀，郭俊明. 长链非编码RNA的作用机制及其研究方法[J]. 遗传, 2013, 35(3): 269-280.

XIA Tian, XIAO Bing-Xiu, GUO Jun-Ming. Acting mechanisms and research methods of long noncoding RNAs[J]. HEREDITAS, 2013, 35(3): 269-280.

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长链非编码RNA的作用机制及其研究方法

Acting mechanisms and research methods of long noncoding RNAs

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