小麦tae-MIR156前体基因的克隆及其靶基因TaSPL17多态性分析

doi:10.3724/SP.J.1005.2014.0592

遗传 ›› 2014, Vol. 36 ›› Issue (6): 592-602.doi: 10.3724/SP.J.1005.2014.0592

小麦tae-MIR156前体基因的克隆及其靶基因TaSPL17多态性分析

刘霞^{1, 2}, 张斌², 毛新国², 李昂², 孙美荣¹, 景蕊莲²

1. 山西大学生物工程学院, 太原 030006;
2. 中国农业科学院作物科学研究所, 农作物基因资源与基因改良国家重大科学工程, 北京 100081

收稿日期:2014-01-09 修回日期:2014-02-11 出版日期:2014-06-20 发布日期:2014-05-28
通讯作者: 景蕊莲,博士,研究员,研究方向：作物抗逆生物学。E-mail:jingruilian@caas.cn E-mail:liuxia1214lx@163.com
作者简介:刘霞,硕士研究生,专业方向：小麦基因资源发掘。E-mail:liuxia1214lx@163.com
基金资助:
国家高技术研究发展计划项目(863计划)(编号：2012AA10A308)资助

Cloning of tae-MIR156 precursor gene and sequence polymorphisms of tae-miR156 targeted TaSPL17

Xia Liu^{1, 2}, Bin Zhang², Xinguo Mao², Ang Li², Meirong Sun², Ruilian Jing²

1. College of Bioengineering, Shanxi University, Taiyuan 030006, China;
2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2014-01-09 Revised:2014-02-11 Online:2014-06-20 Published:2014-05-28

摘要/Abstract

摘要：

Squamosa-promoter binding protein (SBP)-box基因是植物特有的一类转录因子, 广泛参与植物生长发育, 其部分成员受miR156调控。文章克隆了小麦(Triticum aestivum) tae-MIR156前体基因, 转录后能够形成茎环结构。小麦10个SBP-box基因中, 仅TaSPL3和TaSPL17在编码区存在tae-miR156识别位点。SPL17在普通小麦的A基因组供体种乌拉尔图小麦(Triticum urartu, AA) UR209和B基因组供体种拟斯卑尔脱山羊草(Aegilops speltoides, BB) Y2001中均为多拷贝(SPL17-A1、SPL17-A2和SPL17-A3; SPL17-B1、SPL17-B2和SPL17-B3), 在D基因组供体种粗山羊草(Aegilops tauschii, DD) Ae38中仅检测到一种序列(SPL17-D); SPL17-A2与SPL17-B2, SPL17-A3与SPL17-B3、SPL17-D两两之间序列的一致性程度均大于99%, 且与普通小麦(中国春、衡观35和双丰收)的TaSPL17序列具有较高的一致性, 提示它们可能来源于共同的祖先基因, 并且在进化过程中高度保守。靶基因TaSPL17中的tae-miR156识别位点非常保守, 在根据单株穗数和基因型多样性挑选的SubP1和SubP2群体中均未检测到tae-miR156识别位点存在变异碱基。

关键词: miR156, 基因, 多态性分析, 普通小麦

Abstract:

Squamosa-promoter binding protein (SBP)-box genes, encoding plant-specific transcription factors, play an important role in plant development. Some members of the SBP-box gene family are regulated by miR156. In this study, we cloned the tae-MIR156 precursor gene, which could form a stem loop after its transcription. Sequence analysis showed that TaSPL3 and TaSPL17 had putative targets of tae-miR156 among the ten wheat SBP-box genes. The diploid donor species of hexaploid common wheat (Triticum aestivum, genome AABBDD), i.e., Triticum urartu (AA) UR209 and Aegilops speltoides Y2001 (SS, closely related to BB) possessed more than one copy of SPL17 (SPL17-A1, SPL17-A2 and SPL17-A3 from Triticum urartu; SPL17-B1, SPL17-B2 and SPL17-B3 from Aegilops speltoides), while Aegilops tauschii (DD) Ae38 only possessed one (SPL17-D). The identities between nucleotide sequences of SPL17-A2 and SPL17-B2, SPL17-A3 and SPL17-B3 or SPL17-D were higher than 99%. They were highly similar with the sequence of TaSPL17 in common wheat cultivars Chinese Spring, Hengguan 35 and Shuangfengshou. These genes might originate from a common ancestor and were highly conserved in the process of evolution. The target site of tae-miR156 in TaSPL17 was also highly conserved in two subgroups consisted of accessions with diverse spike number per plant and genetic background.

Key words: miR156, gene, polymorphism analysis, common wheat

刘霞, 张斌, 毛新国, 李昂, 孙美荣, 景蕊莲. 小麦tae-MIR156前体基因的克隆及其靶基因TaSPL17多态性分析[J]. 遗传, 2014, 36(6): 592-602.

Xia Liu, Bin Zhang, Xinguo Mao, Ang Li, Meirong Sun, Ruilian Jing. Cloning of tae-MIR156 precursor gene and sequence polymorphisms of tae-miR156 targeted TaSPL17[J]. HEREDITAS(Beijing), 2014, 36(6): 592-602.

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小麦tae-MIR156前体基因的克隆及其靶基因TaSPL17多态性分析

Cloning of tae-MIR156 precursor gene and sequence polymorphisms of tae-miR156 targeted TaSPL17

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