遗传 ›› 1995, Vol. 17 ›› Issue (6): 4-6.

• 论文 • 上一篇    下一篇

云南小麦、西藏半野生小麦和普通小麦叶绿体DNA限制性内切酶图谱的研究

彭隽敏; 孔青; 徐乃瑜 PENG Jun-min;KONG Qing;XU Nai-Yu   

  1. 武汉大学生命科学学院, 武汉 430072 The School of Life Sciences,Wuhan University,Wuhan 430072
  • 收稿日期:1900-01-01 出版日期:1995-12-10 发布日期:1995-12-10

Study on the Restriction Fragment Patterns of Chloroplast DNAsof Yunnan Wheat,Tibet Semiwild Wheat and Common Wheat

  • Received:1900-01-01 Online:1995-12-10 Published:1995-12-10

摘要: 云南小麦(Triticum aestivum ssp.yunnanense King)和西藏半野生小麦(Triticum aestivum ssp.tibetanum Shao) 是我国西部地区特有的两个普通小麦(T.aestivum L.)亚种。我们采用改进的高离子强度法,提取云南小麦、西藏半野生小麦、普通小麦的两个品种(中国春和鄂恩1号)的叶绿体DNA, 并用7种限制性内切酶对它们的叶绿体DNA进行了酶切图谱分析。结果表明:在普通小麦及其两个亚种中没有叶绿体DNA的限制性片段长度差异,反映了叶绿体基因组在进化过程中相对的稳定性。
Abstract:Yunnan wheat(Triticum aestivum ssp. Yunnanense King) and Tibet semiwild wheat (Triticum aestivum ssp. Tibetanum Shao) are two endemic subspecies of common wheat (T. eastivum L.) in the west of China.Improved high ionic strength medium was adopted to extract the cpDNAs of Yunnan wheat,Tibet semiwild wheat and two cultivars of common wheat (Chinese Spring and Een 1).Restriction fragment analysis of cpDNAs was carried out with the use of 7 restriction enzymes.The results showed that there was no diversity of cpDNAs from common wheat and the two subspecies,indicating the conservatism of the chloroplast genome during the process of evolution.

关键词: 叶绿体DNA, 小麦, 限制性内切酶图谱
Key words,
Wheat, cpDNA, 比较, MIVQUE, REML, Monte Carlo, 方差组分

Key words: Variance components, Comparison, MIVQUE, REML, Monte Carlo