遗传 ›› 1999, Vol. 21 ›› Issue (1): 4-5.

• 论文 • 上一篇    下一篇

条纹斑竹鲨基因组的RAPD分析初报

吴冰;陈元霖;桂慕燕 WU Bing;CHEN Yuan-Lin;GUI Mu-Yan   

  1. 福建省厦门大学细胞生物学研究室, 厦门 361005 Laboratory of Cell Biology,Xiamen University,Xiamen 361005
  • 收稿日期:1900-01-01 出版日期:1999-02-10 发布日期:1999-02-10

Preliminary Report on the Genome RAPD Analysis of the Chiloscyllium Plagiosum

  • Received:1900-01-01 Online:1999-02-10 Published:1999-02-10

摘要: 采用11种随机引物对4 条条纹斑竹鲨基因组进行了RAPD检测。结果表明, 11种引物在每条个体上扩增的 DNA片段总数在77~84之间, 单个随机引物扩增的DNA片段数目由1至11条不等,平均为7.5条 DNA 片段, 片段的大小在 300~2 800bp之间。个体之间的相似率在90%以上。
Abstracts 4 individuals of Chiloscyllium plagiosum were analyzed by RAPD method using 11 arbitrary primers. For the 11 arbitrary primers, each individual showed 77~84 bands corresponding to amplified products. Each primer gave 1~11 bands for each individual. On average, about 7.5 bands were obtained per primer per individual. The length of the fragment is 300~2 800 bp. The similarity between band profiles of the four individuals was over 90%.



关键词: RAPD, Genome,  RAPD分析 Key words Chiloscyllium plagiosum,  基因组DNA, 条纹斑竹鲨, 克隆, 受体, 可溶性, 白细胞介素1, 表达

Key words: Interleukin-1 receptor, Insect cells, Gene cloning and expression