遗传 ›› 1999, Vol. 21 ›› Issue (4): 1-5.

• 论文 •    下一篇

人癌细胞线粒体DNA控制区序列特征分析

胡义德;钱桂生;李淑平;陈维中;毛宝龄 HU Yide;QIAN Gui-sheng;LI Shu-ping;CHEN Wei-zhong;MAO Bao-ling   

  1. 第三军医大学新桥医院全军呼吸内科研究所,重庆400037 Institute of Respiratory Diseases of Peoples Liberation Army,Xinqiao Hospital, Third Military Medical University,Chongqing400037,China
  • 收稿日期:1900-01-01 出版日期:1999-08-10 发布日期:1999-08-10

Sequence Feature Analysis of Mitochondrial DNA Control Region in Human Carcinoma Cells

  • Received:1900-01-01 Online:1999-08-10 Published:1999-08-10

摘要: 为了探讨癌细胞mtDNA控制区序列的变化特征, 采用PCR产物限制性片段长度多态性(PCR-RFLP)分析与直接测序相结合的方法,对比分析6株人癌细胞系、 6例癌患者及4例健康成人白细胞mtDNA控制区序列。发现第16519位T→C、16 534位A→G、46位T→G和49位A→C突变, 在癌细胞系和癌患者白细胞mtDNA中分别占50%(3/6)和33.3%(2/6), 健康成人白细胞mtDNA中未见此类型突变;第16 278位C→T突变,在癌细胞系mtDNA中占50%(3/6),显著高于正常人群mtDNA中此位点的多态性变异。表明癌细胞和癌患者白细胞mtDNA重链复制起点及其 相邻D环区的特征性突变可能与细胞癌变/或癌的易感性有关。
Abstract: To explore the sequence feature of mitochondrial DNA(mtDNA) control region in human carcinoma cells, polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and direct sequence techniques were used to analyze the sequence of mtDNA control region of 6 human carcinoma cell lines versus white blood cells which from 6 tumor patients and 4 normal adults. The T to C mutation at np 16 519, A to G mutation at np 16 534, T to G mutation at np 46, and A to C mutation at np 49 was found in 50% (3/6 cases) of carcinoma cell lines and in 33.3%(2/6 cases) of tumor patients, but it was not found in normal adults. The C to T mutation at np 16 278 was found in 50%(3/6 cases) of carcinoma cell lines, it was significantly higher than that of the polymorphism of normal population. These findings suggest that the typical mutation in the starting area of heavy-strand replication and the first half of D-loop region might probably be associated with carcinogenesis or susceptibility of carcinoma.

关键词: 人癌细胞系, 线粒体DNA, 控制区, 突变
Key words,
核苷酸序列, 乳腺表达, 转基因小鼠, PCR, 基因组G-CSF

Key words: Transgenic mice, Mammary gland expression, Genomic G-CSF, PCR