遗传 ›› 2002, Vol. 24 ›› Issue (1): 100-103.
侯丙凯1;于惠敏2;夏光敏1 HOU Bing-kai1;YU Hui-min2;XIA Guang-min1
摘要: 叶绿体遗传转化是植物基因工程的新方向。本文简要介绍用于叶绿体遗传转化的表达载体的构建方法,涉及同源重组片段、叶绿体特异的启动子和终止子、筛选标记基因,以及目前在叶绿体中已实现表达的外源基因等内容。
Abstract:Chloroplast genetic transformation is a new way of plant genetic engineering.This paper reviews the construction methods of expression vector used in chloroplast genetic transformation.It contains the homologous recombinant fragments,the chloroplast specific promoter and terminater,the selectable marker genes and the interest genes whose expression in chloroplast have been achieved.