孔雀微卫星引物筛选及其遗传多样性分析

遗传 ›› 2006, Vol. 28 ›› Issue (10): 1242-1246.

孔雀微卫星引物筛选及其遗传多样性分析

包文斌¹,²;陈国宏¹;束婧婷¹;徐琪¹;李慧芳³

1. 扬州大学动物科学与技术学院, 扬州225009; 2. 安徽农业大学动物科技学院, 合肥230036; 3. 中国农业科学院家禽研究所, 扬州 225003

出版日期:2006-10-01 发布日期:2006-10-01

Screening of Peafowl Microsatellite Primers and Analysis of Genetic Diversity

BAO Wen-Bin¹;²; CHEN Guo-Hong¹; SHU Jing-Ting¹; XU Qi¹; LI Hui-Fang³

1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2. College of Animal Science and Technology, Anhui Agriculture University, Heifei 230036, China; 3. Institute of Poultry Sciences, Chinese Academy of Agricultural Sciences, Yangzhou 225003, China

Online:2006-10-01 Published:2006-10-01

摘要/Abstract

摘要： 利用29对鸡微卫星标记对孔雀基因组DNA进行种间扩增, 发现14对引物能扩增出特异性条带, 每对引物扩增的平均等位基因数为1.71, 有7对引物具有较丰富的多态性, 其中MCW0080和MCW0098最为理想。蓝孔雀和绿孔雀群体间和群体内的遗传分析结果表明, 绿孔雀和蓝孔雀两个群体的期望杂合度分别为0.7422和0.6943, 群体间的遗传分化系数为0.078, Reynolds’遗传距离和基因流分别为0.0603和3.896, 结果显示这两个孔雀群体的杂合度和遗传多样性水平都很低, 且有相互混杂的趋势。

关键词: 孔雀, 微卫星, 遗传多样性

Abstract: Abstract: The applicability of chicken microsatellite primers to peafowl population was analyzed in the present paper, and the results showed 14 of 29 pairs of microsatellite primers from chicken could amplify peafowl DNA and produce specific allele patterns. A mean of 1.71 alleles was found for each locus. Seven pairs were highly polymorphic, and MCW0080 and MCW0098 were ideal markers for peafowl. Genetic diversity analysis within and between the green peafowl and the blue peafowl populations demonstrated that the expected heterozygosity of two peafowl populations were 0.2482 and 0.2744, respectively. The inbreeding index (FST), Reynolds' genetic distance and gene flow between the two populations were 0.078, 0.0603 and 3.896 respectively. These results indicate that the heterozygosity and the genetic diversity of these two peafowl populations were very low, and suggest a tendency towards intermixing.

中图分类号:

Q953

包文斌，陈国宏，束婧婷，徐琪，李慧芳. 孔雀微卫星引物筛选及其遗传多样性分析[J]. 遗传, 2006, 28(10): 1242-1246.

BAO Wen-Bin, CHEN Guo-Hong, SHU Jing-Ting, XU Qi, LI Hui-Fang. Screening of Peafowl Microsatellite Primers and Analysis of Genetic Diversity[J]. HEREDITAS, 2006, 28(10): 1242-1246.

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