用基因诱捕法制作Ayu17-449基因敲除小鼠模型

遗传 ›› 2006, Vol. 28 ›› Issue (2): 129-132.

用基因诱捕法制作Ayu17-449基因敲除小鼠模型

汤华^1*;唐任宽² ;黄爱龙¹;荒木喜美³ ;山村研一³

1．重庆医科大学教育部感染性疾病分子生物学重点实验室重庆，400016；2.重庆医科大学法医学教研室重庆市，400016；3熊本大学发生医学研究中心脏器形成分野日本，熊本 862-0976

收稿日期:2005-06-06 修回日期:2005-08-15 出版日期:2006-02-10 发布日期:2006-02-10
通讯作者: 汤华

Generation of Ayu17-449 Gene Knockout Mice by Gene Trapping

TANG Hua¹，TANG Ren-Kuan², Huang Ai-Long², ARAKI Kimi³， YAMAMURA Kenichi³

1. Key Laboratory of Molecular Infectious Diseases, Chongqing University of Medical Sciences， Chongqing， 400016, China;2. Department of Forensics, Chongqing University of Medical Sciences， Chongqing，400016 China;3. Department of Developmental Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 862-0976, Japan

Received:2005-06-06 Revised:2005-08-15 Online:2006-02-10 Published:2006-02-10
Contact: TANG Hua

摘要/Abstract

摘要：

为了探明Ayu17-449基因在小鼠生长发育过程中的功能, 用特殊的诱捕载体（Gene trapping vector）导入小鼠ES细胞中，5′RACE、Southern blot方法鉴定成功地单一捕获Ayu17-449基因后，由这种ES制作了Ayu17-449 敲除小鼠并用Northern blot方法该基因在突变小鼠体内的表达。结果在Ayu17-449 敲除小鼠体内，诱捕载体位于Ayu17-449基因的翻译起始密码上游，Ayu17-449基因的转录被抑制。表明Ayu17-449敲除小鼠为分析Ayu17-449基因的功能提供了可靠的实验材料。

关键词: ES细胞, Ayu17-449, 捕获载体

Abstract: We report the generation of Ayu17-449 gene knockout mice in an effort to facilitate studies of its function during mouse development, A special gene trapping vector was transfected into mouse ES cells. The clone with the trapped gene trap was identified by 5′RACE and Southern blot analysis. Ayu17-449 trapped mice were then generated by using this ES clone. In addition, the expression of Ayu17-449 in this mutant line was analyzed with Northern blot. Results showed that in these mutant mice, the trapping vector was located upstream of the Ayu17-449 initiation site, and as a result, the transcription of Ayu17-449 gene was inhibited. Ayu17-449 trapped mice are a useful tool for the analysis of Ayu17-449 gene function in mouse development.

中图分类号:

Q812

汤华，唐任宽，黄爱龙，荒木喜美，山村研一. 用基因诱捕法制作Ayu17-449基因敲除小鼠模型[J]. 遗传, 2006, 28(2): 129-132.

TANG Hua, TANG Ren-Kuan, Huang Ai-Long, ARAKI Kimi, YAMAMURA Kenichi. Generation of Ayu17-449 Gene Knockout Mice by Gene Trapping[J]. HEREDITAS, 2006, 28(2): 129-132.

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