遗传 ›› 2006, Vol. 28 ›› Issue (6): 699-706.

• 研究报告 • 上一篇    下一篇

野生垂穗披碱草种质的醇溶蛋白遗传多样性分析

马 啸,周永红,于海清,张海琴   

  1. 四川农业大学小麦研究所, 四川 都江堰 611830
  • 收稿日期:2005-04-30 修回日期:2005-08-11 出版日期:2006-06-10 发布日期:2006-06-10
  • 通讯作者: 周永红

Genetic Diversity of Gliadin in Wild Germplasm of Elymus nutans Griseb

MA Xiao, ZHOU Yong-Hong, YU Hai-Qing, ZHANG Hai-Qin   

  1. Triticeae Research Institute, Sichuan Agricultural University, Sichuan Dujiangyan, 611830, China
  • Received:2005-04-30 Revised:2005-08-11 Online:2006-06-10 Published:2006-06-10
  • Contact:  Zhou Yong-hong

摘要:




用酸性聚丙烯酰胺凝胶电泳(A-PAGE) 对采集自中国新疆、青海、四川、西藏四省区的33份垂穗披碱草Elymus nutans材料进行醇溶蛋白分析,获得下述结果:(1)供试材料共分离出38条带纹,多态率达92.10%。4个电泳分区(α、β、γ、ω)的平均Shannon指数为0.55,Nei-Li遗传相似系数(GS)变异范围为0.36~0.93,平均值为0.63。这些结果说明供试野生垂穗披碱草材料具有较为丰富的醇溶蛋白遗传多样性。(2)对所有材料的聚类分析和主成分分析发现,在GS值为0.67的水平上供试材料可聚成7个类群,绝大部分来自于相同或相似生态地理环境的材料聚成一类,即醇溶蛋白图谱类型与材料的生态地理环境具有一定的相关性。(3)基于Shannon 多样性指数估算了5个垂穗披碱草地理类群内和类群间的遗传分化,发现类群内遗传变异占总变异的42.94 %,而类群间的遗传变异占总变异的57.06 %。这可能与该草以自花传粉为主的繁育系统有关。(4)对各地理类群基于Nei氏无偏估计的遗传一致度的聚类分析表明,各地理类群间的遗传分化与其所处的地理生态环境具有较高的相关性。


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关键词: 聚类分析, 遗传多样性, 醇溶蛋白, 垂穗披碱草, 遗传分化

Abstract: Acid polyacrylamide gel electrophoresis (A-PAGE) was used to detect the gliadin genetic polymorphism among thirty-three wild accessions of Elymus nutans Griseb collected from four provinces and regions in China (Xinjiang, Qinghai, Sichuan and Tibet). The follows results were obtained. (1) A total of 38 bands were detected in all accessions, 92.1% were polymorphic bands. The average number of Shannon index to four electrophoretic zones was 0.55. The Nei’s genetic similarity coefficient of the tested accessions ranged from 0.36 to 0.94, and the average Nei’s coefficient was 0.63. These results suggested that there was rich genetic polymorphism among the tested wild resources of Elymus nutans Griseb. (2) 33 wild accessions can be clustered into seven groups at GS = 0.67 level on dendrogram. The principal coordinates (PCA) reflected almost the same relationships among the studied materials as shown in cluster analysis. Moreover, the groups from the same origin frequently clustered into one group. The findings implied that a correlation among gliadin patterns, geographical and ecological environment. (3) Genetic differentiation between and within five eco-geographical groups of Elymus nutans was estimated by Shannon’s diversity index, which shown that 42.94% genetic variance existed within group, and 50.76% genetic variance was among groups. This result might be correlative to mainly self-pollinated breeding system of E.nutans. (4) UPGMA cluster analysis based on Nei’s unbiased measures of genetic identity was assayed for five geographical groups of Elymus nutans, indicating that there was a significantly positive correlation between genetic differentiation and geographical habits among the five groups.