遗传 ›› 2017, Vol. 39 ›› Issue (9): 856-862.doi: 10.16288/j.yczz.17-192

• 遗传学教学 • 上一篇    下一篇

基于SSLP分子标记验证遗传学三大定律的教学实践探索与体会

黄雪盈1,2(),范凯1,2,叶炎芳1,2,汪斌1,3,吴为人1,2,兰涛1,2()   

  1. 1 福建农林大学作物遗传育种与综合利用教育部重点实验室，福州350002
    2 福建农林大学作物科学学院，福州350002
    3 福建农林大学生命科学学院，福州350002
  • 收稿日期:2017-05-27 修回日期:2017-08-22 出版日期:2017-09-20 发布日期:2017-10-21
  • 基金资助:
    福建农林大学本科教学改革项目(普通遗传学综合性实验的开发与实践,植物科学与技术专业科教深度融合本科教学模式探索)(111415021,111415002)

Teaching practice and experiences of verifying the three laws of genetics based on the SSLP marker analysis

Xueying Huang1,2(),Kai Fan1,2,Yanfang Ye1,2,Bin Wang1,3,Weiren Wu1,2,Tao Lan1,2()   

  1. 1 Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    2 College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
    3 College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2017-05-27 Revised:2017-08-22 Online:2017-09-20 Published:2017-10-21
  • Supported by:
    Fujian Agriculture and Forestry University Reform of Undergraduate Teaching(111415021,111415002)

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摘要：

文章对“水稻SSLP分子标记的遗传分析”作为遗传学实验教学案例的实施过程及其效果进行了探讨。利用位于水稻两条染色体上的3个SSLP标记,对两亲本及其杂交构建的F2代群体进行单株SSLP标记基因型检测,利用检测所得到的基因型结果验证分离定律、独立分配定律及连锁交换定律等遗传学三大定律。实践证明这不仅有利于加深学生对遗传学三大定律的认识,而且在提高学生实验操作技能和综合分析能力的基础上,还有助于培养学生的科研兴趣和创新意识。同时,对该实验的适用范围以及尚需完善之处做了讨论。此综合性实验也是科研成果转化为本科实验教学的一个有益探索。

关键词: 遗传学实验, 遗传学三大定律, 分子标记, 科研转化教学

Abstract:

We explored the practical effect of the genetic analysis of simple sequence length polymorphism (SSLP) molecular markers in rice in the genetics lab course. Two parents and their F2 population were analyzed and detected with three SSLP molecular markers that located on two chromosomes of the rice genome. The markers’ genotype data were used to verify the three laws of genetics, including segregation, independent assortment and linkage and crossing-over. Our practice has proved not only beneficial to deepen students’ understandings about the three laws of genetics, but also conducive to cultivate students’ interests in research and innovation and improve their skills and comprehensive analysis abilities. At the same time, the application scope of the experiment was discussed. This comprehensive experiment is also useful for the transformation of scientific research achievements into undergraduate experimental teaching.

Key words: genetics lab course, the three laws of genetics, molecular markers, converting the research to the teaching