赫氏颗石藻(Emiliania huxleyi)响应病毒感染的microRNA转录组分析

doi:10.16288/j.yczz.21-164

遗传 ›› 2021, Vol. 43 ›› Issue (11): 1088-1100.doi: 10.16288/j.yczz.21-164

赫氏颗石藻(Emiliania huxleyi)响应病毒感染的microRNA转录组分析

张恩权(), 蔡伟聪, 李桂玲, 李健, 刘静雯()

集美大学食品与生物工程学院,厦门 361000

收稿日期:2021-04-30 修回日期:2021-07-14 出版日期:2021-11-20 发布日期:2021-07-29
通讯作者: 刘静雯 E-mail:chriszhangen@163.com;ljwsbch@163.com
作者简介:张恩权,在读硕士研究生,专业方向：海洋微生物组学。E-mail: chriszhangen@163.com
基金资助:
国家自然科学基金面上项目编号(42076086);国家自然科学基金面上项目编号(31771972);福建省自然科学基金编号资助(2019J01696);福建省自然科学基金编号资助(2020J01676)

Analysis of microRNA expression profile in Emiliania huxleyi in response to virus infection

Enquan Zhang(), Weicong Cai, Guiling Li, Jian Li, Jingwen Liu()

College of Food and Bioengineering, Jimei University, Xiamen 361021, China

Received:2021-04-30 Revised:2021-07-14 Online:2021-11-20 Published:2021-07-29
Contact: Liu Jingwen E-mail:chriszhangen@163.com;ljwsbch@163.com
Supported by:
Supported by National Natural Science Foundation of China Nos(42076086);Supported by National Natural Science Foundation of China Nos(31771972);Fujian Province Natural Science Foundation of China Nos(2019J01696);Fujian Province Natural Science Foundation of China Nos(2020J01676)

摘要/Abstract

摘要：

海洋颗石藻病毒-宿主互作是影响海洋碳、硫生物地化循环及全球气候变化的重要环节。作为大的双链DNA病毒,颗石藻病毒进化出一种“病毒细胞代谢”模式,通过重编程宿主代谢途径以满足其代谢需求,但对这一代谢模式的调控机制尚缺乏足够的认识。MicroRNA (miRNA)作为一种基因表达调控的重要因子,能够通过调控代谢过程中的靶基因表达,从而调节相关代谢通路。本研究采用small RNA测序技术分析病毒感染颗石藻差异表达的miRNA及其靶基因功能,鉴定出26条成熟miRNA (包括2条病毒来源的miRNA),均来自23条新的miRNA前体序列,其中5条miRNA显著差异表达,包括4条上调,1条下调。实时荧光定量PCR (quantitative real-time PCR, qRT-PCR)验证结果与miRNA-seq结果基本一致。功能富集分析显示,5个差异表达的miRNA可能参与调节糖代谢、脂代谢和氨基酸等代谢。此外,差异表达miRNA的表达水平与脂质代谢相关靶基因如ACC-1、SPT、ACOX、ACAT、CERS、ACADS等的表达水平呈负相关,说明这些miRNA可能在病毒感染过程中对宿主的脂质代谢发挥重要的调控作用。

关键词: 赫氏颗石藻, 颗石藻病毒, small RNA测序, microRNA, 脂代谢

Abstract:

The interactions between Emiliania huxleyi and E. huxleyi virus (EhV) regulate marine carbon and sulfur biogeochemical cycle and play a prominent role in global climate change. As a large DNA virus, EhVs have developed a novel “virocell metabolism” model to meet their higher metabolic needs. However, the regulatory mechanism of this metabolic model is still largely unclear. MicroRNAs (miRNAs) can regulate biological pathways through targeting hub genes in the metabolic processes. Here, we performed high-throughput small RNA sequencing to analyse miRNA expression in EhV99B1 infected E. huxleyi BOF92. A total of 26 miRNAs (including 2 virus-derived miRNAs) were identified, including four up-regulated and one down-regulated miRNAs. These results were further validated through quantitative real-time PCR. Functional enrichment analysis showed that five differentially-expressed miRNAs might be involved in the regulation of carbohydrate metabolism, lipid metabolism and amino acid metabolism. Moreover, the expression levels of differentially-expressed miRNAs were negatively correlated with that of several lipid metabolism-related genes, such as ACC-1, SPT, ACOX, ACAT, CERS and ACADS, indicating that these miRNAs might play an important regulatory role in virus-mediated lipid metabolism.

Key words: Emiliania huxleyi, Emiliania huxleyi virus, small RNA sequencing, microRNA, lipid metabolism

张恩权, 蔡伟聪, 李桂玲, 李健, 刘静雯. 赫氏颗石藻(Emiliania huxleyi)响应病毒感染的microRNA转录组分析[J]. 遗传, 2021, 43(11): 1088-1100.

Enquan Zhang, Weicong Cai, Guiling Li, Jian Li, Jingwen Liu. Analysis of microRNA expression profile in Emiliania huxleyi in response to virus infection[J]. Hereditas(Beijing), 2021, 43(11): 1088-1100.

图/表 32

表1

表2

图2

图3

图4

图5

图1

图6

附图1

附表1

成熟miRNA的基本信息"

miRNA ID	miRNA序列	长度 (nt)	表达丰度(FPKM)				基因组位置
miRNA ID	miRNA序列	长度 (nt)	Con_6h	Exp_6h	Con_45h	Exp_45h	基因组位置
NW_005195085.1_38173-5p	cgucggcgcaugugguuagag	21	24.23	20.52	17.56	12.32	Intergenic
NW_005195085.1_38180-3p	cuagucguaguaaguguuuga	21	5.51	2.53	7.48	2.66	Intergenic
NW_005196191.1_35170-3p	cagguguauuugugaggccaa	21	2.45	1.11	3.23	2.23	Intergenic
NW_005196603.1_34298-5p (ehx-miR1-5p)	ccgacccgucccugagccu	19	206.33	71.57	145.54	86.69	intron
NW_005196755.1_34052-3p	cgaggagccgccaccagagcu	21	3.42	1.57	3.56	4.59	Intergenic
NW_005196755.1_34028-5p	cgagaugguacugaagagauc	21	240.47	205..45	266.69	116.88	Intergenic
NW_005196755.1_34019-3p	cuuguccgcgucgccucauga	21	1.45	2.46	1.78	2.56	Intergenic
NW_005197715.1_30571-5p	gagguuggcgcgggcgagc	19	2.47	4.76	7.58	3.45	Exon
NW_005198391.1_27186-5p	cgacaccugcuggcuggcgcc	21	3.12	1.63	1.45	1.73	Exon
NW_005198966.1_24265-3p	aacccucgcucggagccgcgc	21	3.32	1.86	1.73	1.43	Intergenic
NW_005198966.1_24265-5p	uugguucugacgauuugu	18	88106.45	105030.78	101495.53	88208.43	Exon
NW_005199363.1_22042-5p	cguucagggcgccgggcacg	20	5.45	1.78	3.51	1.81	Intergenic
NW_005199378.1_21978-3p	gggcuaugcccgcgcgaguuc	21	1.12	0.79	1.96	1.13	Intergenic
NW_005199378.1_21978-5p	guugacguggccgagugguuaag	23	3124.46	1430.13	1710.79	761.41	Intergenic
NW_005199975.1_17848-3p	cagagccacccuccuggacgc	21	2.73	1.23	1.72	0.00	Intergenic
NW_005200282.1_15947-3p	auguugucgccccaguccg	19	24.56	11.22	17.86	19.76	Exon
NW_005200461.1_14543-5p	gccggcuacgccgccgccgggc	22	3.45	1.37	2.56	1.39	Intergenic
NW_005200496.1_14318-5p (ehx-miR2-5p)	ucgccugucguccgacguucugucuccggc	30	25.64	52.78	14.67	7.35	Exon
NW_005200517.1_13952-3p	cggagaagcgucagacucggc	21	17.56	18.67	13.47	13.43	Intergenic
NW_005200535.1_13863-3p	cagucguguguuuggcguccu	21	1.97	3.45	0.99	1.89	Intergenic
NW_005200600.1_13330-3p (ehx-miR3-3p)	gcgagguuggcgcgggcgagc	21	7.36	17.41	18.22	12.56	Intergenic
NW_005200881.1_10661-3p	cgaacuugucggcgcccgcga	21	4.25	1.47	3.34	1.67	Exon
NW_005201234.1_6668-5p	acuccauggccagggccu	18	5.63	1.29	2.42	1.37	Intergenic
NW_005202428.1_1248-3p	gccacggccgcgccgccgcccgcc	24	2.56	2.79	4.34	5.34	Exon
gi\|73852470\|ref\|NC_007346.1\|_540-3p (ehv-miR1-3p)	guucgauccucgccguggguu	21	0.01	0.01	0.01	61.35	Exon
gi\|73852470\|ref\|NC_007346.1\|_540-5p (ehv-miR2-5p)	cccucguagcucaguggauagagcuc	26	0.01	0.01	0.01	7.64	Exon

附表1

附表2

前体miRNA的完整序列"

前体miRNR ID	长度(nt)	前体miRNA序列
NW_005195085.1_38173	74	cgucggcgcaugugguuagagauacaucuagagauacuucugcauuuuucucuuuccgcacgcaccccgaauuc
NW_005195085.1_38180	60	agauguuacgauaacuagcacauuuagaucauucaugugcuagucguaguaaguguuuga
NW_005196191.1_35170	46	ggguuggggaaacaucugucaaaugcagguguauuugugaggccaa
NW_005196603.1_34298	70	ccgacccgucccugagccucccuguaggucgaccgagacuggcugacaaagcgcguggucggcgucggcg
NW_005196755.1_34019	52	aagaggaagggaugugcgagaaagcaaccugcuuguccgcgucgccucauga
NW_005196755.1_34028	72	cgagaugguacugaagagauccguuacgcaaaagcaugcgcgacuugcacucuuucgugccacucgcucgcc
NW_005196755.1_34052	58	auaacgugugucgggcacucgggcugcuuguacggcucgaggagccgccaccagagcu
NW_005197715.1_30571	47	gagguuggcgcgggcgagcuucgacaucuucucgcggcagcggucga
NW_005198391.1_27186	78	cgacaccugcuggcuggcgccggccgucgccucugccgcgcgcgcgaggcccacgucggcgagguagccguugccgcg
NW_005198966.1_24265	53	uugguucugacgauuuguugcgcgcgcgcaacaacccucgcucggagccgcgc
NW_005199363.1_22042	52	cguucagggcgccgggcacgccuuaggggggcgugcgccgugaugcucgggc
NW_005199378.1_21978	65	guugacguggccgagugguuaaggcgagggacugcuaaucccuugggcuaugcccgcgcgaguuc
NW_005199975.1_17848	86	cuccggcgaggugaggaccucugcacuugugaggaccucugcuuccagcccacaucuuccaggugcagagccacccuccuggacgc
NW_005200282.1_15947	39	agcuggggcagcagcgcagcauguugucgccccaguccg
NW_005200461.1_14543	56	gccggcuacgccgccgccgggccagcccgccucgccgccucaugcguugccgccgc
NW_005200496.1_14318	71	ucgccugucguccgacguucugucuccggcacauuacggugucgucgucguuccgcgagagccauccggac
NW_005200517.1_13952	57	gaggucaacauguuucucguagaacacggugacgugcggagaagcgucagacucggc
NW_005200535.1_13863	70	gggccgggggggcggcgggcgcggggugcggggugcggugcggugcgugcagucguguguuuggcguccu
NW_005200600.1_13330	65	gggccucggccggccggggcggcgaggucgugagacagcacgcugcgagguuggcgcgggcgagc
NW_005200881.1_10661	55	accucgcgcggcaggcggcagacgaagauguugccgaacuugucggcgcccgcga
NW_005201234.1_6668	56	acuccauggccagggccuaucgucuucaagaggagaagccuaccgccgaggaggac
NW_005202428.1_1248	53	cgcagcugcgcgcggccgcggcuaccucggccacggccgcgccgccgcccgcc
gi\|73852470\|ref\|NC_007346.1\|_540	72	cccucguagcucaguggauagagcucuugccuucuaagcaaguggucgcgaguucgauccucgccguggguu

附表2

附表3

26条成熟miRNA与已鉴定miRNA的比对结果"

比对到miRbase数据库中已知miRNA的结果
Query ID (本研究中鉴定的miRNA)	Subject ID (其他已知miRNA)	Identities (%)	Length (nt)	Mismatch	Gap	Query start	Query end	Subject Start	Subject end	E value	Bit score
NW_005196603.1_34298-5p	pal-miR-9987-3p	93.75	16	1	0	2	17	20	5	25	19.6
NW_005196603.1_34298-5p	hsa-miR-6743-5p	83.333	18	3	0	2	19	19	2	80	18
NW_005198966.1_24265-3p	hsa-miR-3183	83.333	18	3	0	4	21	2	19	89	18
NW_005199363.1_22042-5p	aof-miR160a	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	vca-miR160-5p	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	cpa-miR160d	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	mes-miR160f	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	mes-miR160e	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	cme-miR160d	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	bdi-miR160e-5p	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	tcc-miR160a	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	ahy-miR160-5p	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	rco-miR160c	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	sbi-miR160f	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	vvi-miR160b	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	vvi-miR160a	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	mtr-miR160c	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	ptc-miR160f	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	ptc-miR160e-5p	83.333	18	3	0	1	18	18	1	84	18
NW_005199363.1_22042-5p	osa-miR160f-5p	83.333	18	3	0	1	18	18	1	84	18
NW_005199378.1_21978-5p	cel-miR-8189-5p	88.889	18	2	0	6	23	21	4	26	19.8
NW_005200461.1_14543-5p	mdo-miR-12392-3p	88.235	17	2	0	6	22	23	7	52	18.8
NW_005200461.1_14543-5p	bta-miR-11976	88.235	17	2	0	2	18	17	1	52	18.8
NW_005200461.1_14543-5p	bta-miR-11975	88.235	17	2	0	2	18	17	1	52	18.8
NW_005200461.1_14543-5p	hsa-miR-3960	88.235	17	2	0	2	18	17	1	52	18.8
NW_005200461.1_14543-5p	mmu-miR-3960	88.235	17	2	0	2	18	17	1	52	18.8
NW_005200461.1_14543-5p	ssc-miR-4332	100	13	0	0	10	22	5	17	69	18.4
NW_005200535.1_13863-3p	mle-miR-10a-3p	80	20	4	0	2	21	3	22	77	18.2
NW_005202428.1_1248-3p	bta-miR-11976	85	20	3	0	1	20	20	1	24	20.1
NW_005202428.1_1248-3p	bta-miR-11975	85	20	3	0	1	20	20	1	24	20.1
NW_005202428.1_1248-3p	hsa-miR-9899	88.235	17	2	0	7	23	6	22	56	18.8
NW_005202428.1_1248-3p	cja-miR-638	100	13	0	0	12	24	22	10	76	18.4
NW_005202428.1_1248-3p	mmu-miR-5126	77.273	22	5	0	2	23	22	1	76	18.4
NW_005202428.1_1248-3p	mml-miR-638	100	13	0	0	12	24	23	11	76	18.4
NW_005202428.1_1248-3p	mdo-miR-7346-3p	80	20	4	0	5	24	20	1	87	18.2
NW_005195085.1_38173-5p	Vca_miR80	85.714	14	2	0	6	19	4	17	17	15.6
NW_005195085.1_38173-5p	Vca_miR116	90.909	11	1	0	4	14	9	19	41	14.4
NW_005195085.1_38173-5p	Thalassiosira	75	20	4	1	1	19	24	5	47	14.2
NW_005195085.1_38173-5p	miR90_0453	78.571	14	3	0	3	16	6	19	63	13.7
NW_005195085.1_38173-5p	miR90_0414	78.571	14	3	0	3	16	5	18	63	13.7
NW_005195085.1_38173-5p	miR90_0041	78.571	14	3	0	3	16	2	15	63	13.7
NW_005195085.1_38173-5p	miR90_0631	83.333	12	2	0	2	13	7	18	73	13.5
NW_005195085.1_38173-5p	miR90_0284	83.333	12	2	0	2	13	13	2	73	13.5
NW_005195085.1_38173-5p	miR95_0193	83.333	12	2	0	2	13	7	18	73	13.5
NW_005195085.1_38173-5p	miR90_0169	91.667	12	0	1	1	12	11	1	85	13.3
NW_005195085.1_38173-5p	miR95_0076	90	10	1	0	1	10	8	17	85	13.3
NW_005195085.1_38173-5p	CCMP1516-miR8	90	10	1	0	1	10	12	21	85	13.3
NW_005195085.1_38173-5p	miR90_0835	100	8	0	0	1	8	10	17	98	13.1
NW_005195085.1_38173-5p	miR90_0689	100	8	0	0	1	8	12	5	98	13.1
NW_005195085.1_38173-5p	miR90_0682	100	8	0	0	1	8	8	1	98	13.1
NW_005195085.1_38173-5p	miR90_0351	100	8	0	0	1	8	3	10	98	13.1
NW_005195085.1_38173-5p	miR90_0271	100	8	0	0	1	8	2	9	98	13.1
NW_005195085.1_38173-5p	miR90_0220	100	8	0	0	1	8	4	11	98	13.1
NW_005195085.1_38180-3p	ccr-miR5	85.714	14	2	0	5	18	6	19	17	15.6
NW_005195085.1_38180-3p	miR90_0317	84.615	13	2	0	5	17	13	25	35	14.6
NW_005195085.1_38180-3p	miR90_0157	78.571	14	3	0	4	17	1	14	63	13.7
NW_005195085.1_38180-3p	ppu-miR21-5p	83.333	12	2	0	1	12	2	13	73	13.5
NW_005195085.1_38180-3p	miR95_0487	68.421	19	6	0	2	20	19	1	85	13.3
NW_005195085.1_38180-3p	Vca_miR107*	68.421	19	6	0	3	21	19	1	85	13.3
NW_005195085.1_38180-3p	Vca_miR19	90	10	1	0	2	11	1	10	85	13.3
NW_005195085.1_38180-3p	SjapMIR84	90	10	1	0	7	16	4	13	85	13.3
NW_005195085.1_38180-3p	miR90_0817	70.588	17	5	0	4	20	17	1	98	13.1
NW_005195085.1_38180-3p	miR95_0559	70.588	17	5	0	2	18	18	2	98	13.1
NW_005196191.1_35170-3p	miR90_0395	78.571	14	3	0	7	20	18	5	63	13.7
NW_005196191.1_35170-3p	miR90_0538	100	8	0	0	3	10	18	11	98	13.1
NW_005196191.1_35170-3p	miR90_0527	70.588	17	5	0	2	18	23	7	98	13.1
NW_005196191.1_35170-3p	miR90_0448	70.588	17	5	0	2	18	3	19	98	13.1
NW_005196191.1_35170-3p	miR90_0421	100	8	0	0	6	13	1	8	98	13.1
NW_005196191.1_35170-3p	miR95_0114	70.588	17	5	0	2	18	20	4	98	13.1
NW_005196603.1_34298-5p	miR90_0660	87.5	16	2	0	3	18	4	19	3.6	17.7
NW_005196603.1_34298-5p	miR95_0377	100	10	0	0	2	11	14	5	21	15.2
NW_005196603.1_34298-5p	miR95_0344	100	10	0	0	2	11	14	5	21	15.2
NW_005196603.1_34298-5p	miR95_0338	100	10	0	0	2	11	11	2	21	15.2
NW_005196603.1_34298-5p	miR95_0320	100	10	0	0	2	11	10	1	21	15.2
NW_005196603.1_34298-5p	miR90_0701	76.471	17	4	0	2	18	17	1	24	15
NW_005196603.1_34298-5p	Vca_miR13*	76.471	17	4	0	2	18	18	2	24	15
NW_005196603.1_34298-5p	SjapMIR5	80	15	3	0	5	19	19	5	28	14.8
NW_005196603.1_34298-5p	miR90_0905	90.909	11	1	0	7	17	1	11	37	14.4
NW_005196603.1_34298-5p	ppu-miR35-5p	100	9	0	0	11	19	16	8	43	14.2
NW_005196603.1_34298-5p	miR90_0203	75	16	4	0	3	18	4	19	50	13.9
NW_005196603.1_34298-5p	Vca_miR38	77.778	18	3	1	1	17	19	2	50	13.9
NW_005196603.1_34298-5p	miR90_0679	78.571	14	3	0	6	19	2	15	57	13.7
NW_005196603.1_34298-5p	miR90_0274	78.571	14	3	0	1	14	2	15	57	13.7
NW_005196603.1_34298-5p	miR90_0185	78.571	14	3	0	6	19	16	3	57	13.7
NW_005196603.1_34298-5p	SjapMIR6	78.571	14	3	0	6	19	2	15	57	13.7
NW_005196603.1_34298-5p	ppu-miR7-5p	83.333	12	2	0	6	17	18	7	66	13.5
NW_005196603.1_34298-5p	miR95_0036	83.333	12	2	0	3	14	7	18	66	13.5
NW_005196603.1_34298-5p	miR90_0147	90	10	1	0	6	15	10	19	77	13.3
NW_005196603.1_34298-5p	Vca_miR67	90	10	1	0	6	15	10	1	77	13.3
NW_005196603.1_34298-5p	ccr-miR9	68.421	19	6	0	1	19	20	2	77	13.3
NW_005196603.1_34298-5p	SjapMIR26_2	90	10	1	0	7	16	15	6	77	13.3
NW_005196603.1_34298-5p	SjapMIR26_1	90	10	1	0	7	16	15	6	77	13.3
NW_005196603.1_34298-5p	miR90_0579	100	8	0	0	4	11	8	1	89	13.1
NW_005196603.1_34298-5p	miR95_0515	100	8	0	0	10	17	9	2	89	13.1
NW_005196755.1_34052-3p	miR95_0442	82.353	17	3	0	4	20	3	19	7.1	16.9
NW_005196755.1_34052-3p	miR90_0873	92.308	13	1	0	4	16	14	2	9.5	16.5
NW_005196755.1_34052-3p	miR90_0824	92.308	13	1	0	4	16	20	8	9.5	16.5
NW_005196755.1_34052-3p	miR90_0824	90	10	1	0	7	16	14	5	85	13.3
NW_005196755.1_34052-3p	miR90_0764	92.308	13	1	0	4	16	19	7	9.5	16.5
NW_005196755.1_34052-3p	miR90_0764	90	10	1	0	7	16	13	4	85	13.3
NW_005196755.1_34052-3p	miR90_0529	100	10	0	0	7	16	11	2	23	15.2
NW_005196755.1_34052-3p	miR95_0450	80	15	3	0	6	20	18	4	31	14.8
NW_005196755.1_34052-3p	Vca_miR44c	80	15	3	0	7	21	5	19	31	14.8
NW_005196755.1_34052-3p	Vca_miR44b	80	15	3	0	7	21	5	19	31	14.8
NW_005196755.1_34052-3p	Vca_miR44a	80	15	3	0	7	21	5	19	31	14.8
NW_005196755.1_34052-3p	miR90_0922	84.615	13	2	0	1	13	18	6	35	14.6
NW_005196755.1_34052-3p	miR90_0898	84.615	13	2	0	7	19	14	2	35	14.6
NW_005196755.1_34052-3p	miR90_0897	84.615	13	2	0	3	15	17	5	35	14.6
NW_005196755.1_34052-3p	miR90_0866	84.615	13	2	0	1	13	21	9	35	14.6
NW_005196755.1_34052-3p	miR90_0771	84.615	13	2	0	3	15	16	4	35	14.6
NW_005196755.1_34052-3p	miR90_0722	84.615	13	2	0	9	21	17	5	35	14.6
NW_005196755.1_34052-3p	miR95_0441	84.615	13	2	0	8	20	25	13	35	14.6
NW_005196755.1_34052-3p	miR95_0439	84.615	13	2	0	3	15	4	16	35	14.6
NW_005196755.1_34052-3p	miR90_0840	90.909	11	1	0	1	11	11	1	41	14.4
NW_005196755.1_34052-3p	miR90_0520	90.909	11	1	0	5	15	17	7	41	14.4
NW_005196755.1_34052-3p	miR90_0515	100	9	0	0	8	16	10	2	47	14.2
NW_005196755.1_34052-3p	Vca_miR30-5p*	72.222	18	5	0	4	21	20	3	47	14.2
NW_005196755.1_34052-3p	miR90_0460	78.571	14	3	0	7	20	2	15	63	13.7
NW_005196755.1_34052-3p	miR95_0509	78.571	14	3	0	6	19	1	14	63	13.7
NW_005196755.1_34052-3p	miR95_0508	78.571	14	3	0	6	19	8	21	63	13.7
NW_005196755.1_34052-3p	Vca_miR16	78.571	14	3	0	8	21	9	22	63	13.7
NW_005196755.1_34052-3p	miR90_0646	83.333	12	2	0	4	15	12	1	73	13.5
NW_005196755.1_34052-3p	Vca_miR98b	83.333	12	2	0	6	17	17	6	73	13.5
NW_005196755.1_34052-3p	Vca_miR98a	83.333	12	2	0	6	17	17	6	73	13.5
NW_005196755.1_34052-3p	miR90_0862	90	10	1	0	1	10	10	1	85	13.3
NW_005196755.1_34052-3p	miR90_0422	90	10	1	0	8	17	8	17	85	13.3
NW_005196755.1_34052-3p	miR95_0300	90	10	1	0	7	16	5	14	85	13.3
NW_005196755.1_34052-3p	miR95_0254	90	10	1	0	7	16	1	10	85	13.3
NW_005196755.1_34052-3p	miR95_0185	90	10	1	0	7	16	9	18	85	13.3
NW_005196755.1_34052-3p	miR95_0164	90	10	1	0	3	12	1	10	85	13.3
NW_005196755.1_34052-3p	miR95_0044	90	10	1	0	7	16	3	12	85	13.3
NW_005196755.1_34052-3p	ppu-miR7-5p	100	8	0	0	11	18	8	1	98	13.1
NW_005196755.1_34052-3p	miR90_0900	100	8	0	0	7	14	3	10	98	13.1
NW_005196755.1_34052-3p	miR90_0891	100	8	0	0	7	14	3	10	98	13.1
NW_005196755.1_34052-3p	miR90_0876	100	8	0	0	1	8	12	19	98	13.1
NW_005196755.1_34052-3p	miR90_0743	100	8	0	0	1	8	11	18	98	13.1
NW_005196755.1_34052-3p	miR90_0624	100	8	0	0	8	15	9	2	98	13.1
NW_005196755.1_34052-3p	miR90_0535	100	8	0	0	8	15	8	1	98	13.1
NW_005196755.1_34052-3p	miR90_0493	100	8	0	0	7	14	8	1	98	13.1
NW_005196755.1_34052-3p	miR90_0229	70.588	17	5	0	4	20	21	5	98	13.1
NW_005196755.1_34052-3p	miR90_0172	100	8	0	0	7	14	12	5	98	13.1
NW_005196755.1_34052-3p	miR90_0165	100	8	0	0	7	14	12	5	98	13.1
NW_005196755.1_34052-3p	miR90_0108	100	8	0	0	7	14	12	5	98	13.1
NW_005196755.1_34052-3p	miR90_0013	100	8	0	0	7	14	10	17	98	13.1
NW_005196755.1_34052-3p	miR95_0416	100	8	0	0	2	9	7	14	98	13.1
NW_005196755.1_34052-3p	miR95_0381	100	8	0	0	8	15	11	18	98	13.1
NW_005196755.1_34052-3p	miR95_0289	100	8	0	0	7	14	12	5	98	13.1
NW_005196755.1_34052-3p	miR95_0288	100	8	0	0	7	14	12	5	98	13.1
NW_005196755.1_34052-3p	miR95_0275	100	8	0	0	8	15	8	1	98	13.1
NW_005196755.1_34052-3p	miR95_0274	100	8	0	0	8	15	11	18	98	13.1
NW_005196755.1_34052-3p	miR95_0260	100	8	0	0	8	15	5	12	98	13.1
NW_005196755.1_34052-3p	miR95_0248	100	8	0	0	7	14	9	16	98	13.1
NW_005196755.1_34052-3p	miR95_0175	100	8	0	0	8	15	10	17	98	13.1
NW_005196755.1_34052-3p	miR95_0158	100	8	0	0	7	14	7	14	98	13.1
NW_005196755.1_34052-3p	miR95_0102	100	8	0	0	7	14	1	8	98	13.1
NW_005196755.1_34052-3p	miR95_0090	100	8	0	0	7	14	8	1	98	13.1
NW_005196755.1_34052-3p	miR95_0088	100	8	0	0	7	14	8	1	98	13.1
NW_005196755.1_34052-3p	Porphyra	100	8	0	0	6	13	8	1	98	13.1
NW_005196755.1_34052-3p	SjapMIR103	100	8	0	0	8	15	16	9	98	13.1
NW_005196755.1_34028-5p	miR95_0404	88.235	17	0	2	1	17	5	19	35	14.6
NW_005196755.1_34028-5p	miR90_0665	90.909	11	1	0	2	12	7	17	41	14.4
NW_005196755.1_34028-5p	miR90_0317	90.909	11	1	0	4	14	11	21	41	14.4
NW_005196755.1_34028-5p	Vca_miR84	90.909	11	1	0	4	14	14	4	41	14.4
NW_005196755.1_34028-5p	Vca_miR58-5p.1	75	16	4	0	4	19	8	23	55	13.9
NW_005196755.1_34028-5p	Vca_miR19	83.333	12	2	0	6	17	4	15	73	13.5
NW_005196755.1_34028-5p	ppu-miR14-3p	90	10	1	0	12	21	12	21	85	13.3
NW_005196755.1_34028-5p	miR90_0837	90	10	1	0	2	11	5	14	85	13.3
NW_005196755.1_34028-5p	miR90_0676	90	10	1	0	10	19	8	17	85	13.3
NW_005196755.1_34028-5p	miR90_0661	90	10	1	0	2	11	5	14	85	13.3
NW_005196755.1_34028-5p	miR90_0637	90	10	1	0	2	11	11	2	85	13.3
NW_005196755.1_34028-5p	miR90_0634	90	10	1	0	2	11	18	9	85	13.3
NW_005196755.1_34028-5p	miR90_0633	90	10	1	0	2	11	11	2	85	13.3
NW_005196755.1_34028-5p	miR90_0586	90	10	1	0	2	11	14	5	85	13.3
NW_005196755.1_34028-5p	miR90_0578	90	10	1	0	2	11	3	12	85	13.3
NW_005196755.1_34028-5p	miR90_0569	90	10	1	0	4	13	18	9	85	13.3
NW_005196755.1_34028-5p	miR90_0555	90	10	1	0	2	11	20	11	85	13.3
NW_005196755.1_34028-5p	miR90_0537	90	10	1	0	2	11	8	17	85	13.3
NW_005196755.1_34028-5p	miR90_0447	90	10	1	0	2	11	2	11	85	13.3
NW_005196755.1_34028-5p	miR90_0446	90	10	1	0	2	11	2	11	85	13.3
NW_005196755.1_34028-5p	miR90_0413	90	10	1	0	2	11	14	5	85	13.3
NW_005196755.1_34028-5p	miR90_0380	90	10	1	0	2	11	10	19	85	13.3
NW_005196755.1_34028-5p	miR90_0357	90	10	1	0	2	11	11	2	85	13.3
NW_005196755.1_34028-5p	miR90_0347	90	10	1	0	2	11	5	14	85	13.3
NW_005196755.1_34028-5p	miR90_0346	90	10	1	0	2	11	15	6	85	13.3
NW_005196755.1_34028-5p	miR90_0345	90	10	1	0	2	11	4	13	85	13.3
NW_005196755.1_34028-5p	miR90_0343	90	10	1	0	2	11	13	4	85	13.3
NW_005196755.1_34028-5p	miR90_0318	90	10	1	0	2	11	13	4	85	13.3
NW_005196755.1_34028-5p	miR90_0295	90	10	1	0	2	11	14	5	85	13.3
NW_005196755.1_34028-5p	miR90_0291	90	10	1	0	2	11	16	7	85	13.3
NW_005196755.1_34028-5p	miR90_0290	90	10	1	0	2	11	18	9	85	13.3
NW_005196755.1_34028-5p	miR90_0289	90	10	1	0	2	11	16	7	85	13.3
NW_005196755.1_34028-5p	miR90_0288	90	10	1	0	2	11	16	7	85	13.3
NW_005196755.1_34028-5p	miR90_0287	90	10	1	0	2	11	15	6	85	13.3
NW_005196755.1_34028-5p	miR90_0280	90	10	1	0	2	11	1	10	85	13.3
NW_005196755.1_34028-5p	miR90_0234	90	10	1	0	2	11	4	13	85	13.3
NW_005196755.1_34028-5p	miR90_0131	90	10	1	0	2	11	14	5	85	13.3
NW_005196755.1_34028-5p	miR90_0128	90	10	1	0	2	11	21	12	85	13.3
NW_005196755.1_34028-5p	miR90_0127	90	10	1	0	2	11	25	16	85	13.3
NW_005196755.1_34028-5p	miR90_0124	90	10	1	0	2	11	14	5	85	13.3
NW_005196755.1_34028-5p	miR90_0123	90	10	1	0	2	11	10	1	85	13.3
NW_005196755.1_34028-5p	miR90_0122	90	10	1	0	2	11	14	5	85	13.3
NW_005196755.1_34028-5p	miR90_0121	90	10	1	0	2	11	18	9	85	13.3
NW_005196755.1_34028-5p	miR90_0120	90	10	1	0	2	11	20	11	85	13.3
NW_005196755.1_34028-5p	miR90_0111	90	10	1	0	2	11	15	6	85	13.3
NW_005196755.1_34028-5p	miR90_0093	90	10	1	0	2	11	18	9	85	13.3
NW_005196755.1_34028-5p	miR90_0092	90	10	1	0	2	11	19	10	85	13.3
NW_005196755.1_34028-5p	miR90_0078	90	10	1	0	2	11	4	13	85	13.3
NW_005196755.1_34028-5p	miR90_0063	90	10	1	0	2	11	9	18	85	13.3
NW_005196755.1_34028-5p	miR90_0062	90	10	1	0	2	11	5	14	85	13.3
NW_005196755.1_34028-5p	miR90_0061	90	10	1	0	2	11	4	13	85	13.3
NW_005196755.1_34028-5p	miR90_0060	90	10	1	0	2	11	3	12	85	13.3
NW_005196755.1_34028-5p	miR90_0040	90	10	1	0	2	11	9	18	85	13.3
NW_005196755.1_34028-5p	miR90_0021	90	10	1	0	2	11	15	6	85	13.3
NW_005196755.1_34028-5p	miR95_0565	90	10	1	0	2	11	15	6	85	13.3
NW_005196755.1_34028-5p	miR95_0565	100	8	0	0	13	20	18	11	98	13.1
NW_005196755.1_34028-5p	miR95_0564	90	10	1	0	2	11	20	11	85	13.3
NW_005196755.1_34028-5p	miR95_0564	100	8	0	0	13	20	23	16	98	13.1
NW_005196755.1_34028-5p	miR95_0443	90	10	1	0	2	11	11	20	85	13.3
NW_005196755.1_34028-5p	miR95_0305	90	10	1	0	2	11	11	2	85	13.3
NW_005196755.1_34028-5p	miR95_0305	100	8	0	0	13	20	14	7	98	13.1
NW_005196755.1_34028-5p	miR95_0298	90	10	1	0	2	11	11	20	85	13.3
NW_005196755.1_34028-5p	miR95_0259	90	10	1	0	2	11	12	3	85	13.3
NW_005196755.1_34028-5p	miR95_0053	90	10	1	0	2	11	14	5	85	13.3
NW_005196755.1_34028-5p	miR95_0052	90	10	1	0	2	11	13	4	85	13.3
NW_005196755.1_34028-5p	miR95_0051	90	10	1	0	2	11	16	7	85	13.3

附表3

附表3

续1"

NW_005196755.1_34028-5p	miR95_0050	90	10	1	0	2	11	12	3	85	13.3
NW_005196755.1_34028-5p	miR95_0049	90	10	1	0	2	11	19	10	85	13.3
NW_005196755.1_34028-5p	miR95_0026	90	10	1	0	2	11	10	19	85	13.3
NW_005196755.1_34028-5p	miR95_0010	90	10	1	0	2	11	11	2	85	13.3
NW_005196755.1_34028-5p	Vca_miR35-3p*	91.667	12	0	1	10	20	22	11	85	13.3
NW_005196755.1_34028-5p	Porphyra	90	10	1	0	1	10	10	1	85	13.3
NW_005196755.1_34028-5p	miR95_0524	100	8	0	0	4	11	15	8	98	13.1
NW_005196755.1_34028-5p	miR95_0500	100	8	0	0	13	20	12	5	98	13.1
NW_005196755.1_34028-5p	miR95_0480	100	8	0	0	1	8	8	1	98	13.1
NW_005196755.1_34028-5p	miR95_0445	100	8	0	0	13	20	12	5	98	13.1
NW_005196755.1_34028-5p	SjapMIR20	100	8	0	0	6	13	1	8	98	13.1
NW_005196755.1_34019-3p	ppu-miR32-5p	80	20	3	1	2	21	19	1	13	16.1
NW_005196755.1_34019-3p	miR90_0172	76.19	21	4	1	1	21	21	2	23	15.2
NW_005196755.1_34019-3p	miR90_0493	76.471	17	4	0	1	17	17	1	26	15
NW_005196755.1_34019-3p	miR95_0289	76.471	17	4	0	1	17	21	5	26	15
NW_005196755.1_34019-3p	miR95_0090	76.471	17	4	0	1	17	17	1	26	15
NW_005196755.1_34019-3p	miR95_0088	76.471	17	4	0	1	17	17	1	26	15
NW_005196755.1_34019-3p	miR95_0468	80	15	3	0	1	15	16	2	31	14.8
NW_005196755.1_34019-3p	SjapMIR60	90.909	11	1	0	5	15	13	3	41	14.4
NW_005196755.1_34019-3p	miR90_0907	75	16	4	0	2	17	2	17	55	13.9
NW_005196755.1_34019-3p	CCMP1516-miR8	77.778	18	3	1	3	20	21	5	55	13.9
NW_005196755.1_34019-3p	miR90_0839	78.571	14	3	0	4	17	1	14	63	13.7
NW_005196755.1_34019-3p	miR90_0765	78.571	14	3	0	4	17	2	15	63	13.7
NW_005196755.1_34019-3p	miR90_0211	78.571	14	3	0	2	15	16	3	63	13.7
NW_005196755.1_34019-3p	ppu-miR15-5p	83.333	12	2	0	1	12	6	17	73	13.5
NW_005196755.1_34019-3p	miR95_0415	83.333	12	2	0	6	17	1	12	73	13.5
NW_005196755.1_34019-3p	miR95_0394	85.714	14	1	1	9	21	17	4	73	13.5
NW_005196755.1_34019-3p	miR95_0194	83.333	12	2	0	6	17	3	14	73	13.5
NW_005196755.1_34019-3p	miR95_0179	83.333	12	2	0	9	20	21	10	73	13.5
NW_005196755.1_34019-3p	miR95_0032	83.333	12	2	0	1	12	13	2	73	13.5
NW_005196755.1_34019-3p	SjapMIR39	83.333	12	2	0	7	18	10	21	73	13.5
NW_005196755.1_34019-3p	miR90_0804	90	10	1	0	1	10	15	24	85	13.3
NW_005196755.1_34019-3p	miR90_0691	90	10	1	0	1	10	15	24	85	13.3
NW_005196755.1_34019-3p	miR90_0658	90	10	1	0	12	21	15	6	85	13.3
NW_005196755.1_34019-3p	miR90_0658	100	8	0	0	14	21	4	11	98	13.1
NW_005196755.1_34019-3p	miR90_0457	90	10	1	0	6	15	6	15	85	13.3
NW_005196755.1_34019-3p	miR90_0278	90	10	1	0	7	16	6	15	85	13.3
NW_005196755.1_34019-3p	miR95_0325	90	10	1	0	8	17	17	8	85	13.3
NW_005196755.1_34019-3p	miR95_0269	90	10	1	0	8	17	18	9	85	13.3
NW_005196755.1_34019-3p	miR90_0424	100	8	0	0	14	21	1	8	98	13.1
NW_005196755.1_34019-3p	miR95_0444	100	8	0	0	6	13	11	4	98	13.1
NW_005196755.1_34019-3p	Botryococcus	70.588	17	5	0	1	17	17	1	98	13.1
NW_005197715.1_30571-5p	miR90_0709	91.667	12	1	0	8	19	18	7	18	15.4
NW_005197715.1_30571-5p	miR90_0495	100	10	0	0	1	10	18	9	21	15.2
NW_005197715.1_30571-5p	Vca_miR60	73.684	19	5	0	1	19	3	21	21	15.2
NW_005197715.1_30571-5p	miR90_0876	80	15	3	0	4	18	1	15	28	14.8
NW_005197715.1_30571-5p	Vca_miR82	80	15	3	0	4	18	2	16	28	14.8
NW_005197715.1_30571-5p	miR90_0690	84.615	13	2	0	7	19	2	14	32	14.6
NW_005197715.1_30571-5p	miR90_0653	84.615	13	2	0	7	19	1	13	32	14.6
NW_005197715.1_30571-5p	miR90_0211	84.615	13	2	0	5	17	1	13	32	14.6
NW_005197715.1_30571-5p	Vca_miR77	84.615	13	2	0	7	19	15	3	32	14.6
NW_005197715.1_30571-5p	miR90_0487	90.909	11	1	0	9	19	19	9	37	14.4
NW_005197715.1_30571-5p	ppu-miR9-3p	100	9	0	0	2	10	3	11	43	14.2
NW_005197715.1_30571-5p	miR90_0862	100	9	0	0	10	18	15	7	43	14.2
NW_005197715.1_30571-5p	miR90_0840	100	9	0	0	10	18	16	8	43	14.2
NW_005197715.1_30571-5p	miR90_0743	100	9	0	0	10	18	6	14	43	14.2
NW_005197715.1_30571-5p	Porphyra	72.222	18	5	0	1	18	3	20	43	14.2
NW_005197715.1_30571-5p	miR90_0400	75	16	4	0	1	16	16	1	50	13.9
NW_005197715.1_30571-5p	miR90_0016	75	16	4	0	1	16	17	2	50	13.9
NW_005197715.1_30571-5p	miR95_0258	77.778	18	3	1	1	18	19	3	50	13.9
NW_005197715.1_30571-5p	Vca_miR13*	75	16	4	0	4	19	6	21	50	13.9
NW_005197715.1_30571-5p	miR90_0759	78.571	14	3	0	4	17	3	16	57	13.7
NW_005197715.1_30571-5p	SjapMIR60	78.571	14	3	0	6	19	2	15	57	13.7
NW_005197715.1_30571-5p	SjapMIR70	83.333	12	2	0	3	14	2	13	66	13.5
NW_005197715.1_30571-5p	miR90_0809	90	10	1	0	7	16	12	3	77	13.3
NW_005197715.1_30571-5p	miR90_0769	90	10	1	0	7	16	11	2	77	13.3
NW_005197715.1_30571-5p	miR90_0754	90	10	1	0	7	16	9	18	77	13.3
NW_005197715.1_30571-5p	miR95_0143	90	10	1	0	1	10	8	17	77	13.3
NW_005197715.1_30571-5p	SjapMIR102	90	10	1	0	1	10	18	9	77	13.3
NW_005197715.1_30571-5p	ppu-miR4-5p	100	8	0	0	12	19	11	18	89	13.1
NW_005197715.1_30571-5p	miR90_0866	100	8	0	0	11	18	25	18	89	13.1
NW_005197715.1_30571-5p	miR90_0706	100	8	0	0	12	19	18	11	89	13.1
NW_005197715.1_30571-5p	miR90_0610	70.588	17	5	0	3	19	1	17	89	13.1
NW_005197715.1_30571-5p	miR90_0560	100	8	0	0	1	8	13	6	89	13.1
NW_005197715.1_30571-5p	miR90_0314	100	8	0	0	1	8	14	7	89	13.1
NW_005197715.1_30571-5p	miR90_0217	100	8	0	0	1	8	17	24	89	13.1
NW_005197715.1_30571-5p	miR95_0499	70.588	17	5	0	1	17	2	18	89	13.1
NW_005197715.1_30571-5p	miR95_0488	70.588	17	5	0	1	17	1	17	89	13.1
NW_005197715.1_30571-5p	miR95_0401	70.588	17	5	0	1	17	18	2	89	13.1
NW_005198391.1_27186-5p	miR90_0599	73.684	19	5	0	2	20	25	7	23	15.2
NW_005198391.1_27186-5p	miR90_0404	73.684	19	5	0	2	20	3	21	23	15.2
NW_005198391.1_27186-5p	miR95_0479	100	10	0	0	12	21	19	10	23	15.2
NW_005198391.1_27186-5p	miR95_0433	100	10	0	0	12	21	18	9	23	15.2
NW_005198391.1_27186-5p	miR95_0337	73.684	19	5	0	2	20	22	4	23	15.2
NW_005198391.1_27186-5p	miR95_0199	73.684	19	5	0	2	20	6	24	23	15.2
NW_005198391.1_27186-5p	miR95_0197	73.684	19	5	0	2	20	7	25	23	15.2
NW_005198391.1_27186-5p	miR95_0082	73.684	19	5	0	2	20	19	1	23	15.2
NW_005198391.1_27186-5p	miR95_0023	73.684	19	5	0	2	20	25	7	23	15.2
NW_005198391.1_27186-5p	miR95_0019	73.684	19	5	0	2	20	19	1	23	15.2
NW_005198391.1_27186-5p	SjapMIR71	76.471	17	4	0	4	20	20	4	26	15
NW_005198391.1_27186-5p	Vca_miR112	86.667	15	1	1	5	18	16	2	35	14.6
NW_005198391.1_27186-5p	miR90_0778	90.909	11	1	0	3	13	2	12	41	14.4
NW_005198391.1_27186-5p	Vca_miR1d	90.909	11	1	0	3	13	3	13	41	14.4
NW_005198391.1_27186-5p	Vca_miR1c	90.909	11	1	0	3	13	3	13	41	14.4
NW_005198391.1_27186-5p	Vca_miR1b	90.909	11	1	0	3	13	3	13	41	14.4
NW_005198391.1_27186-5p	Vca_miR1a	90.909	11	1	0	3	13	3	13	41	14.4
NW_005198391.1_27186-5p	miR95_0563	100	9	0	0	12	20	3	11	47	14.2
NW_005198391.1_27186-5p	miR95_0522	100	9	0	0	12	20	1	9	47	14.2
NW_005198391.1_27186-5p	miR95_0481	72.222	18	5	0	4	21	1	18	47	14.2
NW_005198391.1_27186-5p	miR95_0335	72.222	18	5	0	3	20	1	18	47	14.2
NW_005198391.1_27186-5p	miR90_0616	75	16	4	0	1	16	2	17	55	13.9
NW_005198391.1_27186-5p	miR95_0537	75	16	4	0	2	17	16	1	55	13.9
NW_005198391.1_27186-5p	miR90_0800	78.571	14	3	0	2	15	4	17	63	13.7
NW_005198391.1_27186-5p	miR90_0753	81.25	16	2	1	4	19	18	4	63	13.7
NW_005198391.1_27186-5p	miR90_0285	78.571	14	3	0	2	15	2	15	63	13.7
NW_005198391.1_27186-5p	miR95_0486	78.571	14	3	0	8	21	1	14	63	13.7
NW_005198391.1_27186-5p	miR95_0263	78.571	14	3	0	1	14	3	16	63	13.7
NW_005198391.1_27186-5p	miR95_0116	78.571	14	3	0	1	14	21	8	63	13.7
NW_005198391.1_27186-5p	miR95_0059	78.571	14	3	0	1	14	20	7	63	13.7
NW_005198391.1_27186-5p	Porphyra	78.571	14	3	0	8	21	1	14	63	13.7
NW_005198391.1_27186-5p	ppu-miR8-5p	83.333	12	2	0	10	21	9	20	73	13.5
NW_005198391.1_27186-5p	miR90_0913	83.333	12	2	0	6	17	2	13	73	13.5
NW_005198391.1_27186-5p	miR90_0730	83.333	12	2	0	7	18	14	3	73	13.5
NW_005198391.1_27186-5p	miR90_0680	83.333	12	2	0	2	13	7	18	73	13.5
NW_005198391.1_27186-5p	miR90_0400	85.714	14	1	1	7	20	18	6	73	13.5
NW_005198391.1_27186-5p	miR90_0258	83.333	12	2	0	6	17	16	5	73	13.5
NW_005198391.1_27186-5p	miR95_0247	83.333	12	2	0	3	14	1	12	73	13.5
NW_005198391.1_27186-5p	CCMP1516-miR5	85.714	14	1	1	7	19	1	14	73	13.5
NW_005198391.1_27186-5p	miR90_0615	90	10	1	0	7	16	1	10	85	13.3
NW_005198391.1_27186-5p	miR90_0548	91.667	12	0	1	5	16	11	1	85	13.3
NW_005198391.1_27186-5p	Vca_miR98b*	90	10	1	0	10	19	14	5	85	13.3
NW_005198391.1_27186-5p	Vca_miR98a*	90	10	1	0	10	19	14	5	85	13.3
NW_005198391.1_27186-5p	SjapMIR39	90	10	1	0	1	10	16	7	85	13.3
NW_005198391.1_27186-5p	miR90_0708	100	8	0	0	10	17	10	17	98	13.1
NW_005198391.1_27186-5p	miR90_0016	100	8	0	0	13	20	14	7	98	13.1
NW_005198391.1_27186-5p	miR95_0485	100	8	0	0	14	21	1	8	98	13.1
NW_005198391.1_27186-5p	PC-5p-179621_3	70.588	17	5	0	2	18	4	20	98	13.1
NW_005198391.1_27186-5p	SjapMIR88	100	8	0	0	8	15	5	12	98	13.1
NW_005198966.1_24265-3p	miR95_0490	85.714	14	2	0	3	16	3	16	17	15.6
NW_005198966.1_24265-3p	miR90_0306	76.471	17	4	0	3	19	25	9	26	15
NW_005198966.1_24265-3p	miR90_0223	84.615	13	2	0	7	19	17	5	35	14.6
NW_005198966.1_24265-3p	miR95_0063	84.615	13	2	0	4	16	7	19	35	14.6
NW_005198966.1_24265-3p	miR90_0916	90.909	11	1	0	4	14	12	2	41	14.4
NW_005198966.1_24265-3p	miR90_0188	70	20	6	0	2	21	22	3	41	14.4
NW_005198966.1_24265-3p	miR90_0640	100	9	0	0	9	17	24	16	47	14.2
NW_005198966.1_24265-3p	miR90_0298	100	9	0	0	9	17	22	14	47	14.2
NW_005198966.1_24265-3p	miR95_0303	72.222	18	5	0	1	18	18	1	47	14.2
NW_005198966.1_24265-3p	miR95_0172	72.222	18	5	0	1	18	18	1	47	14.2
NW_005198966.1_24265-3p	miR95_0117	75	16	4	0	4	19	2	17	55	13.9
NW_005198966.1_24265-3p	Porphyra	75	16	4	0	5	20	20	5	55	13.9
NW_005198966.1_24265-3p	miR90_0017	78.571	14	3	0	5	18	14	1	63	13.7
NW_005198966.1_24265-3p	Vca_miR77	78.571	14	3	0	5	18	4	17	63	13.7
NW_005198966.1_24265-3p	miR95_0292	83.333	12	2	0	7	18	12	1	73	13.5
NW_005198966.1_24265-3p	CCMP1516-miR9	83.333	12	2	0	3	14	5	16	73	13.5
NW_005198966.1_24265-3p	miR90_0477	90	10	1	0	9	18	17	8	85	13.3
NW_005198966.1_24265-3p	miR90_0439	68.421	19	6	0	3	21	24	6	85	13.3
NW_005198966.1_24265-3p	miR90_0186	71.429	21	5	1	1	21	3	22	85	13.3
NW_005198966.1_24265-3p	miR90_0182	90	10	1	0	11	20	2	11	85	13.3
NW_005198966.1_24265-3p	miR95_0165	68.421	19	6	0	3	21	19	1	85	13.3
NW_005198966.1_24265-3p	Vca_miR64	90	10	1	0	12	21	12	21	85	13.3
NW_005198966.1_24265-3p	SjapMIR10	90	10	1	0	12	21	9	18	85	13.3
NW_005198966.1_24265-3p	miR90_0013	70.588	17	5	0	4	20	2	18	98	13.1
NW_005198966.1_24265-3p	miR95_0359	70.588	17	5	0	4	20	2	18	98	13.1
NW_005198966.1_24265-3p	miR95_0326	70.588	17	5	0	4	20	2	18	98	13.1
NW_005198966.1_24265-3p	SjapMIR16	70.588	17	5	0	3	19	17	1	98	13.1
NW_005198966.1_24265-5p	SjapMIR63_2	84.615	13	2	0	2	14	4	16	30	14.6
NW_005198966.1_24265-5p	SjapMIR63_1	84.615	13	2	0	2	14	4	16	30	14.6
NW_005198966.1_24265-5p	miR95_0357	90.909	11	1	0	1	11	14	4	35	14.4
NW_005198966.1_24265-5p	miR95_0012	90.909	11	1	0	8	18	25	15	35	14.4
NW_005198966.1_24265-5p	Vca_miR53b	90.909	11	1	0	2	12	1	11	35	14.4
NW_005198966.1_24265-5p	Vca_miR53a	90.909	11	1	0	2	12	1	11	35	14.4
NW_005198966.1_24265-5p	miR95_0462	75	16	4	0	1	16	19	4	47	13.9
NW_005198966.1_24265-5p	miR90_0669	78.571	14	3	0	2	15	14	1	54	13.7
NW_005198966.1_24265-5p	miR90_0445	78.571	14	3	0	3	16	10	23	54	13.7
NW_005198966.1_24265-5p	miR90_0226	78.571	14	3	0	3	16	14	1	54	13.7
NW_005198966.1_24265-5p	miR95_0136	78.571	14	3	0	3	16	15	2	54	13.7
NW_005198966.1_24265-5p	Vca_miR94b	78.571	14	3	0	4	17	2	15	54	13.7
NW_005198966.1_24265-5p	Vca_miR94a	78.571	14	3	0	4	17	2	15	54	13.7
NW_005198966.1_24265-5p	miR90_0577	83.333	12	2	0	1	12	19	8	63	13.5
NW_005198966.1_24265-5p	miR90_0360	83.333	12	2	0	1	12	7	18	63	13.5
NW_005198966.1_24265-5p	miR90_0160	83.333	12	2	0	1	12	6	17	63	13.5
NW_005198966.1_24265-5p	miR90_0058	83.333	12	2	0	1	12	19	8	63	13.5
NW_005198966.1_24265-5p	miR90_0057	83.333	12	2	0	1	12	13	2	63	13.5
NW_005198966.1_24265-5p	miR90_0056	83.333	12	2	0	1	12	17	6	63	13.5
NW_005198966.1_24265-5p	miR95_0460	83.333	12	2	0	6	17	23	12	63	13.5
NW_005198966.1_24265-5p	miR95_0424	83.333	12	2	0	1	12	1	12	63	13.5
NW_005198966.1_24265-5p	miR95_0423	83.333	12	2	0	1	12	3	14	63	13.5
NW_005198966.1_24265-5p	miR95_0362	83.333	12	2	0	1	12	6	17	63	13.5
NW_005198966.1_24265-5p	miR95_0331	83.333	12	2	0	1	12	25	14	63	13.5
NW_005198966.1_24265-5p	miR95_0318	83.333	12	2	0	1	12	15	4	63	13.5
NW_005198966.1_24265-5p	miR95_0208	83.333	12	2	0	1	12	4	15	63	13.5
NW_005198966.1_24265-5p	miR95_0207	83.333	12	2	0	1	12	2	13	63	13.5
NW_005198966.1_24265-5p	miR95_0206	83.333	12	2	0	1	12	11	22	63	13.5
NW_005198966.1_24265-5p	miR95_0204	83.333	12	2	0	1	12	12	23	63	13.5
NW_005198966.1_24265-5p	miR95_0201	83.333	12	2	0	1	12	9	20	63	13.5
NW_005198966.1_24265-5p	miR95_0200	83.333	12	2	0	1	12	13	24	63	13.5
NW_005198966.1_24265-5p	miR95_0078	83.333	12	2	0	1	12	2	13	63	13.5
NW_005198966.1_24265-5p	miR95_0077	83.333	12	2	0	1	12	6	17	63	13.5
NW_005198966.1_24265-5p	miR95_0071	83.333	12	2	0	1	12	16	5	63	13.5
NW_005198966.1_24265-5p	miR95_0024	83.333	12	2	0	1	12	18	7	63	13.5
NW_005198966.1_24265-5p	miR95_0022	83.333	12	2	0	1	12	12	1	63	13.5
NW_005198966.1_24265-5p	miR95_0018	83.333	12	2	0	1	12	12	1	63	13.5
NW_005198966.1_24265-5p	miR95_0017	83.333	12	2	0	1	12	19	8	63	13.5
NW_005198966.1_24265-5p	Vca_miR84	83.333	12	2	0	2	13	12	1	63	13.5
NW_005198966.1_24265-5p	miR90_0142	90	10	1	0	9	18	20	11	73	13.3
NW_005198966.1_24265-5p	SjapMIR64	90	10	1	0	2	11	4	13	73	13.3
NW_005198966.1_24265-5p	miR95_0383	100	8	0	0	4	11	2	9	84	13.1
NW_005198966.1_24265-5p	Vca_miR88b	73.333	15	4	0	2	16	9	23	97	12.9
NW_005198966.1_24265-5p	Vca_miR88a	73.333	15	4	0	2	16	9	23	97	12.9
NW_005198966.1_24265-5p	Vca_miR68*	73.333	15	4	0	2	16	4	18	97	12.9
NW_005199363.1_22042-5p	miR90_0108	83.333	18	2	1	4	20	2	19	14	15.8
NW_005199363.1_22042-5p	miR90_0013	73.684	19	5	0	2	20	21	3	22	15.2
NW_005199363.1_22042-5p	miR90_0172	82.353	17	2	1	4	19	2	18	29	14.8
NW_005199363.1_22042-5p	miR90_0165	82.353	17	2	1	4	19	2	18	29	14.8
NW_005199363.1_22042-5p	miR95_0448	80	15	3	0	3	17	2	16	29	14.8
NW_005199363.1_22042-5p	miR95_0248	82.353	17	2	1	5	20	18	2	29	14.8
NW_005199363.1_22042-5p	miR95_0158	82.353	17	2	1	4	19	17	1	29	14.8
NW_005199363.1_22042-5p	ppu-miR3-5p	84.615	13	2	0	3	15	1	13	34	14.6
NW_005199363.1_22042-5p	miR90_0457	90.909	11	1	0	6	16	17	7	39	14.4
NW_005199363.1_22042-5p	Vca_miR38	90.909	11	1	0	2	12	2	12	39	14.4
NW_005199363.1_22042-5p	miR90_0428	100	9	0	0	12	20	24	16	45	14.2
NW_005199363.1_22042-5p	miR95_0522	100	9	0	0	3	11	10	18	45	14.2
NW_005199363.1_22042-5p	miR95_0289	72.222	18	5	0	2	19	1	18	45	14.2
NW_005199363.1_22042-5p	miR95_0288	72.222	18	5	0	2	19	1	18	45	14.2
NW_005199363.1_22042-5p	miR95_0541	78.571	14	3	0	5	18	15	2	60	13.7
NW_005199363.1_22042-5p	miR95_0515	78.571	14	3	0	1	14	1	14	60	13.7
NW_005199363.1_22042-5p	miR95_0419	78.571	14	3	0	2	15	16	3	60	13.7
NW_005199363.1_22042-5p	miR95_0415	78.571	14	3	0	1	14	17	4	60	13.7
NW_005199363.1_22042-5p	miR95_0325	78.571	14	3	0	1	14	3	16	60	13.7
NW_005199363.1_22042-5p	miR95_0269	78.571	14	3	0	1	14	4	17	60	13.7
NW_005199363.1_22042-5p	miR95_0246	78.571	14	3	0	1	14	14	1	60	13.7
NW_005199363.1_22042-5p	miR95_0118	78.571	14	3	0	7	20	4	17	60	13.7
NW_005199363.1_22042-5p	miR90_0493	83.333	12	2	0	8	19	3	14	70	13.5
NW_005199363.1_22042-5p	miR95_0468	83.333	12	2	0	8	19	2	13	70	13.5
NW_005199363.1_22042-5p	miR95_0179	83.333	12	2	0	1	12	6	17	70	13.5
NW_005199363.1_22042-5p	miR95_0179	68.421	19	6	0	2	20	24	6	81	13.3
NW_005199363.1_22042-5p	miR95_0090	83.333	12	2	0	8	19	3	14	70	13.5
NW_005199363.1_22042-5p	miR95_0088	83.333	12	2	0	8	19	3	14	70	13.5
NW_005199363.1_22042-5p	SjapMIR56	83.333	12	2	0	1	12	13	2	70	13.5
NW_005199363.1_22042-5p	miR90_0169	90	10	1	0	6	15	9	18	81	13.3
NW_005199363.1_22042-5p	miR95_0188	90	10	1	0	7	16	1	10	81	13.3
NW_005199363.1_22042-5p	SjapMIR61	90	10	1	0	9	18	12	3	81	13.3
NW_005199363.1_22042-5p	miR90_0876	70.588	17	5	0	2	18	1	17	94	13.1
NW_005199363.1_22042-5p	miR90_0726	100	8	0	0	1	8	4	11	94	13.1
NW_005199363.1_22042-5p	miR90_0629	100	8	0	0	1	8	8	15	94	13.1
NW_005199363.1_22042-5p	miR95_0563	100	8	0	0	3	10	12	19	94	13.1
NW_005199363.1_22042-5p	miR95_0388	100	8	0	0	1	8	7	14	94	13.1
NW_005199363.1_22042-5p	miR95_0387	100	8	0	0	1	8	14	21	94	13.1
NW_005199363.1_22042-5p	miR95_0354	100	8	0	0	1	8	3	10	94	13.1
NW_005199363.1_22042-5p	miR95_0133	100	8	0	0	1	8	10	17	94	13.1
NW_005199363.1_22042-5p	miR95_0111	100	8	0	0	1	8	6	13	94	13.1
NW_005199363.1_22042-5p	miR95_0087	100	8	0	0	1	8	15	22	94	13.1
NW_005199363.1_22042-5p	miR95_0083	100	8	0	0	1	8	8	15	94	13.1
NW_005199363.1_22042-5p	miR95_0080	100	8	0	0	1	8	9	16	94	13.1
NW_005199378.1_21978-3p	ppu-miR5-3p	73.684	19	5	0	3	21	20	2	23	15.2
NW_005199378.1_21978-3p	Vca_miR22	84.615	13	2	0	1	13	13	1	35	14.6
NW_005199378.1_21978-3p	SjapMIR95	90.909	11	1	0	3	13	16	6	41	14.4
NW_005199378.1_21978-3p	Vca_miR88b*	100	9	0	0	3	11	17	9	47	14.2
NW_005199378.1_21978-3p	Vca_miR88a*	100	9	0	0	3	11	17	9	47	14.2
NW_005199378.1_21978-3p	miR90_0844	75	16	4	0	1	16	9	24	55	13.9
NW_005199378.1_21978-3p	miR95_0514	75	16	4	0	1	16	9	24	55	13.9
NW_005199378.1_21978-3p	miR95_0390	75	16	4	0	1	16	4	19	55	13.9
NW_005199378.1_21978-3p	miR95_0346	75	16	4	0	1	16	3	18	55	13.9
NW_005199378.1_21978-3p	miR95_0324	75	16	4	0	1	16	5	20	55	13.9
NW_005199378.1_21978-3p	miR90_0701	78.571	14	3	0	1	14	1	14	63	13.7
NW_005199378.1_21978-3p	miR95_0369	78.571	14	3	0	6	19	19	6	63	13.7
NW_005199378.1_21978-3p	PC-3p-33398_31	83.333	12	2	0	2	13	12	1	73	13.5
NW_005199378.1_21978-3p	miR90_0567	90	10	1	0	6	15	16	7	85	13.3
NW_005199378.1_21978-3p	miR90_0513	90	10	1	0	6	15	11	2	85	13.3
NW_005199378.1_21978-3p	miR90_0012	90	10	1	0	6	15	10	1	85	13.3
NW_005199378.1_21978-3p	miR95_0558	100	8	0	0	1	8	9	16	98	13.1
NW_005199378.1_21978-5p(ehx-miR2-5p)	PC-3p-33398_31	92.857	14	1	0	6	19	6	19	5	17.5
NW_005199378.1_21978-5p(ehx-miR2-5p)	PC-3p-11444_150	92.308	13	1	0	7	19	7	19	10	16.5
NW_005199378.1_21978-5p(ehx-miR2-5p)	CCMP1516-miR18	80	20	3	1	1	19	2	21	14	16.1
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0571	80	15	3	0	3	17	16	2	33	14.8
NW_005199378.1_21978-5p(ehx-miR2-5p)	Vca_miR60	80	15	3	0	1	15	6	20	33	14.8
NW_005199378.1_21978-5p(ehx-miR2-5p)	Vca_miR32*	80	15	3	0	1	15	7	21	33	14.8
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0599	90.909	11	1	0	10	20	11	1	45	14.4
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0378	90.909	11	1	0	5	15	17	7	45	14.4
NW_005199378.1_21978-5p(ehx-miR2-5p)	ppu-miR15-5p	75	16	4	0	4	19	17	2	60	13.9
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0701	75	16	4	0	4	19	3	18	60	13.9
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0663	75	16	4	0	5	20	18	3	60	13.9
NW_005199378.1_21978-5p(ehx-miR2-5p)	ppu-miR38-3p	78.571	14	3	0	7	20	2	15	69	13.7
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0535	78.571	14	3	0	1	14	2	15	69	13.7
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0515	78.571	14	3	0	1	14	4	17	69	13.7
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR95_0279	78.571	14	3	0	1	14	16	3	69	13.7
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR95_0167	78.571	14	3	0	1	14	18	5	69	13.7
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0651	83.333	12	2	0	1	12	2	13	80	13.5
NW_005199378.1_21978-5p(ehx-miR2-5p)	SjapMIR2	66.667	21	7	0	1	21	1	21	80	13.5
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR90_0342	90	10	1	0	6	15	19	10	93	13.3
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR95_0507	90	10	1	0	3	12	15	24	93	13.3
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR95_0451	90	10	1	0	3	12	8	17	93	13.3
NW_005199378.1_21978-5p(ehx-miR2-5p)	miR95_0210	90	10	1	0	3	12	9	18	93	13.3
NW_005199975.1_17848-3p	miR90_0607	88.235	17	1	1	5	20	18	2	8.2	16.7
NW_005199975.1_17848-3p	miR90_0778	92.857	14	0	1	7	19	1	14	20	15.4
NW_005199975.1_17848-3p	miR95_0547	91.667	12	1	0	6	17	3	14	20	15.4
NW_005199975.1_17848-3p	miR95_0545	91.667	12	1	0	6	17	3	14	20	15.4
NW_005199975.1_17848-3p	miR90_0411	80	15	3	0	1	15	2	16	31	14.8
NW_005199975.1_17848-3p	miR90_0352	86.667	15	1	1	3	17	4	17	35	14.6
NW_005199975.1_17848-3p	miR90_0293	86.667	15	1	1	6	19	18	4	35	14.6
NW_005199975.1_17848-3p	miR90_0258	84.615	13	2	0	6	18	20	8	35	14.6
NW_005199975.1_17848-3p	miR90_0011	86.667	15	1	1	6	19	17	3	35	14.6
NW_005199975.1_17848-3p	miR90_0884	92.308	13	0	1	7	19	3	14	41	14.4
NW_005199975.1_17848-3p	miR90_0704	92.308	13	0	1	7	19	4	15	41	14.4
NW_005199975.1_17848-3p	miR90_0583	92.308	13	0	1	7	19	10	21	41	14.4
NW_005199975.1_17848-3p	miR90_0545	92.308	13	0	1	7	19	13	24	41	14.4
NW_005199975.1_17848-3p	miR90_0509	92.308	13	0	1	7	19	7	18	41	14.4
NW_005199975.1_17848-3p	miR90_0431	92.308	13	0	1	7	19	7	18	41	14.4
NW_005199975.1_17848-3p	miR90_0427	92.308	13	0	1	7	19	13	2	41	14.4
NW_005199975.1_17848-3p	miR90_0415	92.308	13	0	1	7	19	13	24	41	14.4
NW_005199975.1_17848-3p	miR90_0292	92.308	13	0	1	7	19	9	20	41	14.4
NW_005199975.1_17848-3p	miR90_0277	92.308	13	0	1	7	19	5	16	41	14.4
NW_005199975.1_17848-3p	miR90_0276	92.308	13	0	1	7	19	6	17	41	14.4
NW_005199975.1_17848-3p	miR90_0139	92.308	13	0	1	7	19	16	5	41	14.4
NW_005199975.1_17848-3p	miR90_0125	90.909	11	1	0	1	11	5	15	41	14.4
NW_005199975.1_17848-3p	miR90_0113	92.308	13	0	1	7	19	20	9	41	14.4
NW_005199975.1_17848-3p	miR90_0081	92.308	13	0	1	7	19	5	16	41	14.4
NW_005199975.1_17848-3p	miR90_0071	92.308	13	0	1	7	19	2	13	41	14.4
NW_005199975.1_17848-3p	miR90_0070	92.308	13	0	1	7	19	3	14	41	14.4
NW_005199975.1_17848-3p	miR90_0069	92.308	13	0	1	7	19	3	14	41	14.4
NW_005199975.1_17848-3p	miR90_0046	92.308	13	0	1	7	19	18	7	41	14.4
NW_005199975.1_17848-3p	miR90_0026	92.308	13	0	1	7	19	3	14	41	14.4
NW_005199975.1_17848-3p	miR90_0022	92.308	13	0	1	7	19	17	6	41	14.4
NW_005199975.1_17848-3p	miR90_0002	92.308	13	0	1	7	19	1	12	41	14.4
NW_005199975.1_17848-3p	miR90_0001	92.308	13	0	1	7	19	1	12	41	14.4
NW_005199975.1_17848-3p	miR90_0847	72.222	18	5	0	3	20	19	2	47	14.2
NW_005199975.1_17848-3p	miR90_0441	72.222	18	5	0	1	18	4	21	47	14.2
NW_005199975.1_17848-3p	Vca_miR52	100	9	0	0	9	17	18	10	47	14.2
NW_005199975.1_17848-3p	SjapMIR103	72.222	18	5	0	1	18	18	1	47	14.2
NW_005199975.1_17848-3p	miR90_0846	78.571	14	3	0	1	14	6	19	63	13.7
NW_005199975.1_17848-3p	miR95_0513	78.571	14	3	0	1	14	1	14	63	13.7
NW_005199975.1_17848-3p	miR95_0502	78.571	14	3	0	7	20	19	6	63	13.7
NW_005199975.1_17848-3p	SjapMIR11	78.571	14	3	0	4	17	14	1	63	13.7
NW_005199975.1_17848-3p	miR95_0537	83.333	12	2	0	3	14	18	7	73	13.5
NW_005199975.1_17848-3p	miR95_0512	83.333	12	2	0	3	14	1	12	73	13.5
NW_005199975.1_17848-3p	miR95_0179	83.333	12	2	0	10	21	16	5	73	13.5
NW_005199975.1_17848-3p	miR90_0795	90	10	1	0	10	19	2	11	85	13.3
NW_005199975.1_17848-3p	miR90_0698	90	10	1	0	3	12	13	4	85	13.3
NW_005199975.1_17848-3p	miR90_0685	90	10	1	0	3	12	13	4	85	13.3
NW_005199975.1_17848-3p	miR90_0660	90	10	1	0	11	20	17	8	85	13.3
NW_005199975.1_17848-3p	miR90_0268	90	10	1	0	10	19	1	10	85	13.3
NW_005199975.1_17848-3p	miR90_0267	90	10	1	0	10	19	2	11	85	13.3
NW_005199975.1_17848-3p	miR90_0082	90	10	1	0	10	19	1	10	85	13.3
NW_005199975.1_17848-3p	miR95_0543	90	10	1	0	2	11	15	6	85	13.3
NW_005199975.1_17848-3p	miR95_0120	90	10	1	0	9	18	5	14	85	13.3
NW_005199975.1_17848-3p	miR95_0100	90	10	1	0	9	18	8	17	85	13.3
NW_005199975.1_17848-3p	miR90_0316	70.588	17	5	0	3	19	2	18	98	13.1
NW_005199975.1_17848-3p	Vca_miR68*	100	8	0	0	1	8	11	4	98	13.1
NW_005200282.1_15947-3p	Vca_miR32	77.778	18	4	0	1	18	19	2	12	16.1
NW_005200282.1_15947-3p	miR95_0534	91.667	12	1	0	2	13	4	15	18	15.4
NW_005200282.1_15947-3p	miR95_0532	91.667	12	1	0	2	13	3	14	18	15.4
NW_005200282.1_15947-3p	miR95_0538	100	10	0	0	2	11	9	18	21	15.2

附表3

附表3

续2"

比对其他微藻miRNA的结果
Query ID (本研究中鉴定的miRNA)	Subject ID (其他已知miRNA)	Identities (%)	Length (nt)	Mismatch	Gap	Query start	Query end	Subject Start	Subject end	E value	Bit score
NW_005200282.1_15947-3p	miR95_0479	76.471	17	4	0	3	19	18	2	24	15
NW_005200282.1_15947-3p	miR95_0433	76.471	17	4	0	3	19	17	1	24	15
NW_005200282.1_15947-3p	miR90_0584	80	15	3	0	3	17	15	1	28	14.8
NW_005200282.1_15947-3p	miR95_0485	80	15	3	0	5	19	2	16	28	14.8
NW_005200282.1_15947-3p	SjapMIR11	90.909	11	1	0	9	19	13	3	37	14.4
NW_005200282.1_15947-3p	Vca_miR1d	100	9	0	0	7	15	18	10	43	14.2
NW_005200282.1_15947-3p	Vca_miR1c	100	9	0	0	7	15	18	10	43	14.2
NW_005200282.1_15947-3p	Vca_miR1b	100	9	0	0	7	15	18	10	43	14.2
NW_005200282.1_15947-3p	Vca_miR1a	100	9	0	0	7	15	18	10	43	14.2
NW_005200282.1_15947-3p	miR90_0568	78.571	14	3	0	3	16	14	1	57	13.7
NW_005200282.1_15947-3p	miR90_0412	78.571	14	3	0	3	16	2	15	57	13.7
NW_005200282.1_15947-3p	miR90_0018	78.571	14	3	0	2	15	14	1	57	13.7
NW_005200282.1_15947-3p	ppu-miR4-5p	83.333	12	2	0	7	18	16	5	66	13.5
NW_005200282.1_15947-3p	miR90_0576	83.333	12	2	0	8	19	18	7	66	13.5
NW_005200282.1_15947-3p	miR90_0529	83.333	12	2	0	2	13	15	4	66	13.5
NW_005200282.1_15947-3p	miR95_0415	85.714	14	1	1	6	19	5	17	66	13.5
NW_005200282.1_15947-3p	miR95_0325	85.714	14	1	1	6	19	15	3	66	13.5
NW_005200282.1_15947-3p	miR95_0269	85.714	14	1	1	6	19	16	4	66	13.5
NW_005200282.1_15947-3p	miR95_0254	83.333	12	2	0	1	12	10	21	66	13.5
NW_005200282.1_15947-3p	miR95_0246	85.714	14	1	1	6	19	2	14	66	13.5
NW_005200282.1_15947-3p	miR95_0149	83.333	12	2	0	1	12	5	16	66	13.5
NW_005200282.1_15947-3p	miR95_0179	90	10	1	0	4	13	22	13	77	13.3
NW_005200282.1_15947-3p	Vca_miR88b*	90	10	1	0	9	18	12	3	77	13.3
NW_005200282.1_15947-3p	Vca_miR88a*	90	10	1	0	9	18	12	3	77	13.3
NW_005200282.1_15947-3p	Vca_miR45	90	10	1	0	3	12	21	12	77	13.3
NW_005200282.1_15947-3p	SjapMIR39	90	10	1	0	5	14	12	21	77	13.3
NW_005200282.1_15947-3p	miR90_0445	70.588	17	5	0	3	19	18	2	89	13.1
NW_005200282.1_15947-3p	miR90_0402	70.588	17	5	0	3	19	25	9	89	13.1
NW_005200282.1_15947-3p	miR90_0239	100	8	0	0	4	11	6	13	89	13.1
NW_005200282.1_15947-3p	miR95_0527	100	8	0	0	2	9	9	16	89	13.1
NW_005200282.1_15947-3p	miR95_0497	100	8	0	0	2	9	10	17	89	13.1
NW_005200282.1_15947-3p	miR95_0484	70.588	17	5	0	2	18	18	2	89	13.1
NW_005200282.1_15947-3p	miR95_0251	100	8	0	0	8	15	23	16	89	13.1
NW_005200282.1_15947-3p	miR95_0194	100	8	0	0	6	13	7	14	89	13.1
NW_005200282.1_15947-3p	miR95_0002	100	8	0	0	2	9	11	18	89	13.1
NW_005200461.1_14543-5p	miR90_0642	80	20	4	0	1	20	1	20	3.1	18.2
NW_005200461.1_14543-5p	miR90_0642	90.909	11	1	0	9	19	3	13	43	14.4
NW_005200461.1_14543-5p	miR90_0642	90	10	1	0	10	19	1	10	89	13.3

附表3

附表3

续3"

比对其他微藻miRNA的结果
Query ID (本研究中鉴定的miRNA)	Subject ID (其他已知miRNA)	Identities (%)	Length (nt)	Mismatch	Gap	Query start	Query end	Subject Start	Subject end	E value	Bit score
NW_005200461.1_14543-5p	miR90_0592	80	20	4	0	1	20	1	20	3.1	18.2
NW_005200461.1_14543-5p	miR90_0592	90.909	11	1	0	9	19	3	13	43	14.4
NW_005200461.1_14543-5p	miR90_0592	90	10	1	0	10	19	1	10	89	13.3
NW_005200461.1_14543-5p	miR90_0220	87.5	16	2	0	5	20	18	3	4.1	17.7
NW_005200461.1_14543-5p	miR90_0657	92.857	14	1	0	6	19	1	14	4.8	17.5
NW_005200461.1_14543-5p	miR90_0657	77.778	18	4	0	3	20	1	18	13	16.1
NW_005200461.1_14543-5p	miR90_0641	92.857	14	1	0	6	19	1	14	4.8	17.5
NW_005200461.1_14543-5p	miR90_0641	77.778	18	4	0	3	20	1	18	13	16.1
NW_005200461.1_14543-5p	miR90_0621	92.857	14	1	0	6	19	1	14	4.8	17.5
NW_005200461.1_14543-5p	miR90_0621	77.778	18	4	0	3	20	1	18	13	16.1
NW_005200461.1_14543-5p	miR90_0727	100	12	0	0	8	19	1	12	5.5	17.3
NW_005200461.1_14543-5p	miR90_0727	91.667	12	1	0	9	20	5	16	21	15.4
NW_005200461.1_14543-5p	miR90_0580	100	12	0	0	8	19	2	13	5.5	17.3
NW_005200461.1_14543-5p	miR90_0580	91.667	12	1	0	9	20	6	17	21	15.4
NW_005200461.1_14543-5p	miR90_0580	90	10	1	0	10	19	1	10	89	13.3
NW_005200461.1_14543-5p	miR90_0434	78.947	19	4	0	1	19	19	1	6.4	17.1
NW_005200461.1_14543-5p	miR90_0557	82.353	17	3	0	6	22	18	2	7.4	16.9
NW_005200461.1_14543-5p	miR95_0455	82.353	17	3	0	3	19	17	1	7.4	16.9
NW_005200461.1_14543-5p	miR90_0466	86.667	15	2	0	6	20	4	18	8.6	16.7
NW_005200461.1_14543-5p	miR90_0356	86.667	15	2	0	6	20	4	18	8.6	16.7
NW_005200461.1_14543-5p	miR90_0442	77.778	18	4	0	1	18	18	1	13	16.1
NW_005200461.1_14543-5p	miR90_0536	81.25	16	3	0	1	16	18	3	15	15.8
NW_005200461.1_14543-5p	miR90_0536	100	9	0	0	12	20	10	2	50	14.2
NW_005200461.1_14543-5p	miR90_0141	81.25	16	3	0	1	16	19	4	15	15.8
NW_005200461.1_14543-5p	miR90_0141	100	9	0	0	12	20	11	3	50	14.2
NW_005200461.1_14543-5p	miR95_0004	81.25	16	3	0	1	16	19	4	15	15.8
NW_005200461.1_14543-5p	miR95_0004	100	9	0	0	12	20	11	3	50	14.2
NW_005200461.1_14543-5p	miR90_0777	85.714	14	2	0	1	14	5	18	18	15.6
NW_005200461.1_14543-5p	miR90_0777	83.333	12	2	0	8	19	3	14	77	13.5
NW_005200461.1_14543-5p	miR90_0271	85.714	14	2	0	6	19	18	5	18	15.6
NW_005200461.1_14543-5p	miR90_0271	72.222	18	5	0	3	20	18	1	50	14.2
NW_005200461.1_14543-5p	miR90_0271	75	16	4	0	1	16	3	18	57	13.9
NW_005200461.1_14543-5p	miR90_0189	85.714	14	2	0	6	19	6	19	18	15.6
NW_005200461.1_14543-5p	miR90_0104	85.714	14	2	0	6	19	21	8	18	15.6
NW_005200461.1_14543-5p	miR90_0104	73.684	19	5	0	3	21	21	3	24	15.2
NW_005200461.1_14543-5p	miR90_0104	78.571	14	3	0	3	16	8	21	66	13.7
NW_005200461.1_14543-5p	miR95_0185	85.714	14	2	0	5	18	4	17	18	15.6
NW_005200461.1_14543-5p	miR95_0185	90.909	11	1	0	9	19	5	15	43	14.4

附表3

附表3

续4"

比对其他微藻miRNA的结果
Query ID (本研究中鉴定的miRNA)	Subject ID (其他已知miRNA)	Identities (%)	Length (nt)	Mismatch	Gap	Query start	Query end	Subject Start	Subject end	E value	Bit score
NW_005200461.1_14543-5p	miR95_0076	85.714	14	2	0	6	19	2	15	18	15.6
NW_005200461.1_14543-5p	miR95_0076	84.615	13	2	0	8	20	1	13	37	14.6
NW_005200461.1_14543-5p	miR95_0076	78.571	14	3	0	6	19	18	5	66	13.7
NW_005200461.1_14543-5p	miR90_0689	91.667	12	1	0	9	20	5	16	21	15.4
NW_005200461.1_14543-5p	miR90_0689	83.333	12	2	0	2	13	4	15	77	13.5
NW_005200461.1_14543-5p	miR90_0407	91.667	12	1	0	9	20	2	13	21	15.4
NW_005200461.1_14543-5p	miR90_0351	91.667	12	1	0	8	19	17	6	21	15.4
NW_005200461.1_14543-5p	miR90_0351	83.333	12	2	0	9	20	13	2	77	13.5
NW_005200461.1_14543-5p	miR90_0215	91.667	12	1	0	9	20	1	12	21	15.4
NW_005200461.1_14543-5p	miR90_0898	100	10	0	0	9	18	15	6	24	15.2
NW_005200461.1_14543-5p	miR90_0898	90.909	11	1	0	9	19	18	8	43	14.4
NW_005200461.1_14543-5p	miR90_0477	100	10	0	0	11	20	1	10	24	15.2
NW_005200461.1_14543-5p	miR90_0477	90	10	1	0	9	18	2	11	89	13.3
NW_005200461.1_14543-5p	miR95_0444	100	10	0	0	8	17	17	8	24	15.2
NW_005200461.1_14543-5p	miR95_0444	83.333	12	2	0	10	21	18	7	77	13.5
NW_005200461.1_14543-5p	miR95_0439	100	10	0	0	10	19	8	17	24	15.2
NW_005200461.1_14543-5p	miR95_0439	100	9	0	0	9	17	10	18	50	14.2
NW_005200461.1_14543-5p	miR95_0439	90	10	1	0	10	19	5	14	89	13.3
NW_005200461.1_14543-5p	miR95_0300	100	10	0	0	9	18	4	13	24	15.2
NW_005200461.1_14543-5p	miR95_0300	90.909	11	1	0	9	19	1	11	43	14.4
NW_005200461.1_14543-5p	miR95_0176	100	10	0	0	10	19	20	11	24	15.2
NW_005200461.1_14543-5p	miR95_0044	100	10	0	0	9	18	2	11	24	15.2
NW_005200461.1_14543-5p	miR95_0044	100	9	0	0	11	19	1	9	50	14.2
NW_005200461.1_14543-5p	miR95_0006	100	10	0	0	10	19	1	10	24	15.2
NW_005200461.1_14543-5p	Vca_miR44c	73.684	19	5	0	2	20	21	3	24	15.2
NW_005200461.1_14543-5p	Vca_miR44c	78.571	14	3	0	9	22	17	4	66	13.7
NW_005200461.1_14543-5p	Vca_miR44b	73.684	19	5	0	2	20	21	3	24	15.2
NW_005200461.1_14543-5p	Vca_miR44b	78.571	14	3	0	9	22	17	4	66	13.7
NW_005200461.1_14543-5p	Vca_miR44a	73.684	19	5	0	2	20	21	3	24	15.2
NW_005200461.1_14543-5p	Vca_miR44a	78.571	14	3	0	9	22	17	4	66	13.7
NW_005200461.1_14543-5p	miR90_0182	76.471	17	4	0	1	17	17	1	28	15
NW_005200461.1_14543-5p	miR90_0208	82.353	17	2	1	4	19	1	17	32	14.8
NW_005200461.1_14543-5p	miR90_0208	90	10	1	0	9	18	10	19	89	13.3
NW_005200461.1_14543-5p	miR90_0809	84.615	13	2	0	7	19	1	13	37	14.6
NW_005200461.1_14543-5p	miR90_0809	83.333	12	2	0	9	20	6	17	77	13.5
NW_005200461.1_14543-5p	miR90_0564	84.615	13	2	0	6	18	6	18	37	14.6
NW_005200461.1_14543-5p	miR90_0254	84.615	13	2	0	7	19	17	5	37	14.6
NW_005200461.1_14543-5p	miR90_0223	84.615	13	2	0	6	18	13	1	37	14.6

附表3

引物名称	序列(5′→3′)	长度(nt)
U6-F	CTCGCTTCGGCAGCACA	17
U6-R	AACGCTTCACGAATTTGCGT	20
ehx-miR1-5p-F	ATTCCGACCCGTCCCTGA	18
ehx-miR2-5p-F	GTACTCGCCTGTCGTCCG	18
ehx-miR3-3p-F	GCGAGGTTGGCGCGGGC	17
ehv-miR1-3p-F	CTAAGTTCGATCCTCGCC	18
ehv-miR2-5p-F	CCCTCGTAGCTCAGTGGA	18
miRNA universal reverse primer	GTGCAGGGTCCGAGGT	16
CDKA-F	TACGCCGACGAGGACTAC	18
CDKA-R	GCGATATGCTTGGGCAGG	18
ACC-1-F	GAACCGGGTGGATGCAGT	18
ACC-1-R	GAAGGAACATGGCCTTGT	18
SPT-F	TCTCGGACACGCTCAACC	18
SPT-R	CCCTCGACGATGATGATG	18
ACOX-F	GTCCGCTTCACCGTCCAG	18
ACOX-R	CGACGATCAGTCCAGACA	18
ACAT-F	GTCAACAAGGTCTGCTCG	18
ACAT-R	AGGTAGTACGGCACGTTG	18
CERS-F	GAGGAGCGGCACAAGGA	17
CERS-R	CCGATGCGGACAAAGTTG	18
ACADS-F	GAGCACGGGCAACCTCAT	18
ACADS-R	ATGTCGGCGAGCAGGAAC	18

实验组别	miRNA	log₂ (fold change)	-ΔΔCt
Exp_6h vs Con_6h	ehx-miR1-5p	-1.271**	-1.44±0.31*
Exp_6h vs Con_6h	ehx-miR2-5p	1.082*	2.15±0.68**
Exp_6h vs Con_6h	ehx-miR3-3p	1.545**	1.88±0.59**
Exp_45h vs Con_45h	ehv-miR1-5p	8.484^a, **	24.24±1.12^b, **
Exp_45h vs Con_45h	ehv-miR2-3p	11.60^a, **	30.12±2.08^b, **

赫氏颗石藻(Emiliania huxleyi)响应病毒感染的microRNA转录组分析

Analysis of microRNA expression profile in Emiliania huxleyi in response to virus infection

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