遗传 ›› 2006, Vol. 28 ›› Issue (11): 1457-1461.doi: 10.1360/yc-006-1457

• 专论与综述 • 上一篇    下一篇

高效siRNA设计的研究进展

许德晖; 黄 辰; 刘利英; 宋土生   

  1. 西安交通大学医学院遗传学与分子生物学系/环境与疾病相关基因教育部重点实验室, 西安 710061

  • 收稿日期:2006-01-13 修回日期:2006-03-17 出版日期:2006-11-05 发布日期:2006-11-05
  • 通讯作者: 宋土生

New Progress of the Highly Efficient siRNA Design

XU De-Hui; HUANG Chen; LIU Li-Ying; SONG Tu-Sheng   

  1. Department of Genetics and Molecular Biology, College of Medicine of JiaoTong University/Key Laboratory of Environment and Dis-ease-Related Gene, Education Ministry, Xi’an 710061, China
  • Received:2006-01-13 Revised:2006-03-17 Online:2006-11-05 Published:2006-11-05
  • Contact: SONG Tu-Sheng

摘要:

RNA干扰(RNA interference, RNAi) 是生物界普遍存在的一种抵御外来基因和病毒感染的保守进化机制, 其本质是siRNA与靶向mRNA特异结合、并由RISC介导其降解, 从而阻止mRNA的翻译, 导致基因沉默。因此, RNAi可以作为基因功能研究、基因治疗等的新工具。但是, 随机设计的siRNA之间沉默效应差别很大。如何针对靶基因设计特异、高效的siRNA就成了一个关键的问题。文章对siRNA设计原则的研究进展进行了总结论述。

关键词: siRNA, RNAi, 序列设计

Abstract:

RNA interference (RNAi), a conserved evolutionary mechanism widely found in living nature, is considered for defending the alien gene and virus infection. RNAi can induce gene silencing by prohibiting the translation of the target mRNA, which is specially recognized by the corresponding siRNA, and finally degraded by the RISC. So RNAi can be applied as a new technology tool for the functional gene research and gene therapy. But the effects of random designed siRNAs on gene silencing differ greatly from each other. The design of the special, highly efficient siRNA comes to be a crucial problem. This issue gives an overview about the new progress of the siRNA design principle.

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